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The map label for this gene is nagB [H]

Identifier: 195977792

GI number: 195977792

Start: 681033

End: 681737

Strand: Direct

Name: nagB [H]

Synonym: Sez_0658

Alternate gene names: 195977792

Gene position: 681033-681737 (Clockwise)

Preceding gene: 195977790

Following gene: 195977793

Centisome position: 33.65

GC content: 43.83

Gene sequence:

>705_bases
ATGAAAATTATTCGTGTACAGGATCAGTTAGAGGGTGGCAAGGTTGCTTTTAGCCTATTAAAGGAAAGCTTGGCAGAAGG
GGCTACAACCTTAGGTTTGGCAACAGGCAGCACACCGATTACCTTTTATCAGGAGCTGGTTAATAGTGATTTAGACTGTT
CAGCCTTGACCAGTATCAACTTAGACGAATATGTCGGGCTGCCGGTTGAAAATGATCAGAGCTATGACTATTTTATGCGT
GATCAGCTGTTTAACGCTAAGCCCTTTAAGGAAAGCTTTTTACCAAACGGCTTAGCTGATGACTTAGAGGCAGAGGTTAA
GCGCTATGATCAAGTTATCGCAGAGCACCCTATTGATTTTCAGATTCTTGGAATTGGGCGCAATGGTCATATCGGCTTTA
ACGAGCCAGGAACCTCTTTTGCAGAAAAGACGCATGTGGTGGATTTGCAGGCTTCGACAATTGAGGCTAATAGTCGCTTC
TTTGCTAGTATTGATGATGTGCCAAAGCAGGCTATTTCAATGGGAATTGCTTCTATCATGGCATCAAAAATGATTGTCCT
ATTGGCCTTTGGTAAGGAAAAGGCTGCCGCTATTAAGGGCATGGTATCAGGTCCAGTTACTGAGGCTTTGCCAGCAAGTG
TATTACAGCAGCACGATAATGTTGTGGTTATTATTGATGAGGCTGCCGCTTCAGAGCTGGATTAG

Upstream 100 bases:

>100_bases
AGCTGATGACAAGGGATATGAGATTATGGCTTGACAATAATTGGTATATACCATATAATAGAACCACAAAAGGTCTATAC
CAATCAAAAAGGAGAATCAG

Downstream 100 bases:

>100_bases
ACTATTGCTGATGGCTAACAGCTAGTCTGCTATCCGTTGTGCTCTTTTGATACAGTGTAGGTATCTGGCTAAAGGTTTCT
TGCTGACTTTAGCAAAAAAT

Product: glucosamine-6-phosphate deaminase NagB

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKIIRVQDQLEGGKVAFSLLKESLAEGATTLGLATGSTPITFYQELVNSDLDCSALTSINLDEYVGLPVENDQSYDYFMR
DQLFNAKPFKESFLPNGLADDLEAEVKRYDQVIAEHPIDFQILGIGRNGHIGFNEPGTSFAEKTHVVDLQASTIEANSRF
FASIDDVPKQAISMGIASIMASKMIVLLAFGKEKAAAIKGMVSGPVTEALPASVLQQHDNVVVIIDEAAASELD

Sequences:

>Translated_234_residues
MKIIRVQDQLEGGKVAFSLLKESLAEGATTLGLATGSTPITFYQELVNSDLDCSALTSINLDEYVGLPVENDQSYDYFMR
DQLFNAKPFKESFLPNGLADDLEAEVKRYDQVIAEHPIDFQILGIGRNGHIGFNEPGTSFAEKTHVVDLQASTIEANSRF
FASIDDVPKQAISMGIASIMASKMIVLLAFGKEKAAAIKGMVSGPVTEALPASVLQQHDNVVVIIDEAAASELD
>Mature_234_residues
MKIIRVQDQLEGGKVAFSLLKESLAEGATTLGLATGSTPITFYQELVNSDLDCSALTSINLDEYVGLPVENDQSYDYFMR
DQLFNAKPFKESFLPNGLADDLEAEVKRYDQVIAEHPIDFQILGIGRNGHIGFNEPGTSFAEKTHVVDLQASTIEANSRF
FASIDDVPKQAISMGIASIMASKMIVLLAFGKEKAAAIKGMVSGPVTEALPASVLQQHDNVVVIIDEAAASELD

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=248, Percent_Identity=34.2741935483871, Blast_Score=136, Evalue=1e-32,
Organism=Homo sapiens, GI19923881, Length=213, Percent_Identity=35.6807511737089, Blast_Score=129, Evalue=2e-30,
Organism=Escherichia coli, GI1786893, Length=211, Percent_Identity=36.4928909952607, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1789530, Length=200, Percent_Identity=30.5, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI48994958, Length=232, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17554876, Length=212, Percent_Identity=36.3207547169811, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581960, Length=212, Percent_Identity=39.1509433962264, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19920764, Length=212, Percent_Identity=39.1509433962264, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 25300; Mature: 25300

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIRVQDQLEGGKVAFSLLKESLAEGATTLGLATGSTPITFYQELVNSDLDCSALTSIN
CEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHCCCC
LDEYVGLPVENDQSYDYFMRDQLFNAKPFKESFLPNGLADDLEAEVKRYDQVIAEHPIDF
HHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
QILGIGRNGHIGFNEPGTSFAEKTHVVDLQASTIEANSRFFASIDDVPKQAISMGIASIM
EEEEECCCCCCCCCCCCCCHHHHHEEEEEECCEEECCCEEEECHHHHHHHHHHHHHHHHH
ASKMIVLLAFGKEKAAAIKGMVSGPVTEALPASVLQQHDNVVVIIDEAAASELD
HHHHEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKIIRVQDQLEGGKVAFSLLKESLAEGATTLGLATGSTPITFYQELVNSDLDCSALTSIN
CEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHCCCC
LDEYVGLPVENDQSYDYFMRDQLFNAKPFKESFLPNGLADDLEAEVKRYDQVIAEHPIDF
HHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
QILGIGRNGHIGFNEPGTSFAEKTHVVDLQASTIEANSRFFASIDDVPKQAISMGIASIM
EEEEECCCCCCCCCCCCCCHHHHHEEEEEECCEEECCCEEEECHHHHHHHHHHHHHHHHH
ASKMIVLLAFGKEKAAAIKGMVSGPVTEALPASVLQQHDNVVVIIDEAAASELD
HHHHEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA