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The map label for this gene is lacE [H]

Identifier: 195977601

GI number: 195977601

Start: 481207

End: 482889

Strand: Direct

Name: lacE [H]

Synonym: Sez_0460

Alternate gene names: 195977601

Gene position: 481207-482889 (Clockwise)

Preceding gene: 195977600

Following gene: 195977602

Centisome position: 23.77

GC content: 43.26

Gene sequence:

>1683_bases
ATGAACAAGCTAATTGAACACATTGAAAAGAGAAAGCCTTTATTTGAGAAGATTTCTCGAAACATTTACCTACGTGCTAT
TCGTGATGGCTTTATTGCAGGTATGCCGGTCATTTTGTTTTCAAGTATCTTTATTTTACTGGCTTATGTTCCAAATGCTT
GGGGCTTTCATTGGAGTAAGTCTGTTGAAGGCTTACTGATGCTGCCTTATAGCTACTCGATGGGTATTCTTGGCTTTTTT
GTTGCCGGAACAACAGCCAAGGCCTTGACTGACTCGATCAATCGTCAGCTAGAAGCCACCAATCAGATGAATTATTTATC
AACCATGCTTGCTGCAATGGTTGGCTTTCTACTAATGGCTGCTGATCCTGCAAAAGAAGGCGGTCTTCTAACAGGCTTCA
TGGGAACAAAGGGACTTTTGACTGCCTTTATTTCCGCCTTTGTGACTGTCACCATTTATAAGCTTTGTGTTAAAAATCAT
GTGACCATTCGCATGCCTGATGAGGTACCACCTAACATTTCACAGGTATTTAAGGATTTGATCCCATTTACCTTATCTAT
TGTTTTACTTTATCTTGTGCAGCTGATTGTTAAGCACACCTTGGGTGTTAATGTTGCTGAGTCCATCGGTGCCTTATTAG
CGCCATTGTTTCAGGCTGCAGATGGGTATTTGGGAATCACGCTTATTTTTGGAGCTTATGCTTTCTTTTGGTTTGTGGGA
ATTCATGGCCCATCTATTGTTGAGCCGGCAATTGCAGCGATTACCTATGCCAATGCTGAGGTCAATCTCAATCTTTTGCA
GGCAGGTCAGCATGCTGATAAGATCCTCACCTCTGGAACACAAATGTTTATTGTAACAATGGGCGGTACTGGAGCCACTC
TTGTTGTGCCATTTATGTTTATGTGGCTAACCAAATCAAAACGCAACCGAGCTATTGGACGTGCCTCTGTTGTACCTACG
TTCTTTGGGGTTAATGAGCCGATACTCTTTGGTGCTCCGCTGGTGTTAAATCCGATTTTCTTTATTCCTTTTATTTTGAC
ACCGATTGTTAATGTTTGGATCTTTAAATTCTTCATTGATGTTTTGGGAATGAATAGCTTTACAGCCAACCTGCCTTGGA
CAACGCCAGCTCCGCTTGGCTTGGTATTAGGAACGAACCTTCAGGTGCTGTCCTTTGTCCTTGCTGCTGTGTTGATTGCT
GTTGATGTTTTGATTTATTATCCTTTCTTAAAGGTCTATGATGAGCAAATCCTTGCTGAGGAGATAGCTGGAAATACCTC
TCATCAATCTCTAACAGATAAGGTAGCTGCTAGCTTTGATACTAAAAAAGCGCAGGCTATTCTTCAAAAGCACCAACCAA
TCACCGCAGAAGCTAATATCCTTGTGCTTTGTGCAGGTGGTGGAACAAGCGGCCTTTTGGCCAATGCGCTGACAAAGGCT
GCCAAGGAATACCAAGCACCAATCAAGGCTGCTGCAGGCAGCTACGGTGCTCACAGGGAGATATTGTCGCAATATCAGCT
GGTTATCCTAGCCCCACAGGTTGCTTCTAATTATGAGGACATCAAGCTAGAGACAGACAAGCTAGGCATTAAGCTAGCCA
AGACAGAAGGAGCAGAATACATCAGACTGACGCGTGATGGTCAGGCTGCCTTGGCTTTTGTGCAAAGAGAGCTAGAAAAC
TAG

Upstream 100 bases:

>100_bases
TAGTGTTACCTTGATGCATGGGCAGGACCATTTGATGACAACTTTGCTGCTGCAGGATATGATGAAGCACCTTATTGAGC
TTTACAAGAGAGGATAAGAC

Downstream 100 bases:

>100_bases
GAGGTCATCATGATCAAACGATTACCTGACGATTTCATTTTTGGTGGTGCTACAGCAGCTTATCAGGCTGAGGGTGCCAC
AACAATAGACGGTAAGGGAG

Product: PTS system lactose-specific EIICB component LacE

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MNKLIEHIEKRKPLFEKISRNIYLRAIRDGFIAGMPVILFSSIFILLAYVPNAWGFHWSKSVEGLLMLPYSYSMGILGFF
VAGTTAKALTDSINRQLEATNQMNYLSTMLAAMVGFLLMAADPAKEGGLLTGFMGTKGLLTAFISAFVTVTIYKLCVKNH
VTIRMPDEVPPNISQVFKDLIPFTLSIVLLYLVQLIVKHTLGVNVAESIGALLAPLFQAADGYLGITLIFGAYAFFWFVG
IHGPSIVEPAIAAITYANAEVNLNLLQAGQHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILTPIVNVWIFKFFIDVLGMNSFTANLPWTTPAPLGLVLGTNLQVLSFVLAAVLIA
VDVLIYYPFLKVYDEQILAEEIAGNTSHQSLTDKVAASFDTKKAQAILQKHQPITAEANILVLCAGGGTSGLLANALTKA
AKEYQAPIKAAAGSYGAHREILSQYQLVILAPQVASNYEDIKLETDKLGIKLAKTEGAEYIRLTRDGQAALAFVQRELEN

Sequences:

>Translated_560_residues
MNKLIEHIEKRKPLFEKISRNIYLRAIRDGFIAGMPVILFSSIFILLAYVPNAWGFHWSKSVEGLLMLPYSYSMGILGFF
VAGTTAKALTDSINRQLEATNQMNYLSTMLAAMVGFLLMAADPAKEGGLLTGFMGTKGLLTAFISAFVTVTIYKLCVKNH
VTIRMPDEVPPNISQVFKDLIPFTLSIVLLYLVQLIVKHTLGVNVAESIGALLAPLFQAADGYLGITLIFGAYAFFWFVG
IHGPSIVEPAIAAITYANAEVNLNLLQAGQHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILTPIVNVWIFKFFIDVLGMNSFTANLPWTTPAPLGLVLGTNLQVLSFVLAAVLIA
VDVLIYYPFLKVYDEQILAEEIAGNTSHQSLTDKVAASFDTKKAQAILQKHQPITAEANILVLCAGGGTSGLLANALTKA
AKEYQAPIKAAAGSYGAHREILSQYQLVILAPQVASNYEDIKLETDKLGIKLAKTEGAEYIRLTRDGQAALAFVQRELEN
>Mature_560_residues
MNKLIEHIEKRKPLFEKISRNIYLRAIRDGFIAGMPVILFSSIFILLAYVPNAWGFHWSKSVEGLLMLPYSYSMGILGFF
VAGTTAKALTDSINRQLEATNQMNYLSTMLAAMVGFLLMAADPAKEGGLLTGFMGTKGLLTAFISAFVTVTIYKLCVKNH
VTIRMPDEVPPNISQVFKDLIPFTLSIVLLYLVQLIVKHTLGVNVAESIGALLAPLFQAADGYLGITLIFGAYAFFWFVG
IHGPSIVEPAIAAITYANAEVNLNLLQAGQHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILTPIVNVWIFKFFIDVLGMNSFTANLPWTTPAPLGLVLGTNLQVLSFVLAAVLIA
VDVLIYYPFLKVYDEQILAEEIAGNTSHQSLTDKVAASFDTKKAQAILQKHQPITAEANILVLCAGGGTSGLLANALTKA
AKEYQAPIKAAAGSYGAHREILSQYQLVILAPQVASNYEDIKLETDKLGIKLAKTEGAEYIRLTRDGQAALAFVQRELEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=450, Percent_Identity=25.3333333333333, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61026; Mature: 61026

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLIEHIEKRKPLFEKISRNIYLRAIRDGFIAGMPVILFSSIFILLAYVPNAWGFHWSK
CCHHHHHHHHCCHHHHHHCCCEEEEHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SVEGLLMLPYSYSMGILGFFVAGTTAKALTDSINRQLEATNQMNYLSTMLAAMVGFLLMA
CCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADPAKEGGLLTGFMGTKGLLTAFISAFVTVTIYKLCVKNHVTIRMPDEVPPNISQVFKDL
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
IPFTLSIVLLYLVQLIVKHTLGVNVAESIGALLAPLFQAADGYLGITLIFGAYAFFWFVG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IHGPSIVEPAIAAITYANAEVNLNLLQAGQHADKILTSGTQMFIVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCEEEEHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
MWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFILTPIVNVWIFKFFID
HHHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGMNSFTANLPWTTPAPLGLVLGTNLQVLSFVLAAVLIAVDVLIYYPFLKVYDEQILAE
HHCCCCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAGNTSHQSLTDKVAASFDTKKAQAILQKHQPITAEANILVLCAGGGTSGLLANALTKA
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
AKEYQAPIKAAAGSYGAHREILSQYQLVILAPQVASNYEDIKLETDKLGIKLAKTEGAEY
HHHHHCHHHHHCCCCCHHHHHHHCCCEEEECCHHHCCCCCEEEEHHHCCEEEEECCCCCE
IRLTRDGQAALAFVQRELEN
EEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNKLIEHIEKRKPLFEKISRNIYLRAIRDGFIAGMPVILFSSIFILLAYVPNAWGFHWSK
CCHHHHHHHHCCHHHHHHCCCEEEEHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SVEGLLMLPYSYSMGILGFFVAGTTAKALTDSINRQLEATNQMNYLSTMLAAMVGFLLMA
CCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADPAKEGGLLTGFMGTKGLLTAFISAFVTVTIYKLCVKNHVTIRMPDEVPPNISQVFKDL
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
IPFTLSIVLLYLVQLIVKHTLGVNVAESIGALLAPLFQAADGYLGITLIFGAYAFFWFVG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IHGPSIVEPAIAAITYANAEVNLNLLQAGQHADKILTSGTQMFIVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCEEEEHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
MWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFILTPIVNVWIFKFFID
HHHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGMNSFTANLPWTTPAPLGLVLGTNLQVLSFVLAAVLIAVDVLIYYPFLKVYDEQILAE
HHCCCCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAGNTSHQSLTDKVAASFDTKKAQAILQKHQPITAEANILVLCAGGGTSGLLANALTKA
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
AKEYQAPIKAAAGSYGAHREILSQYQLVILAPQVASNYEDIKLETDKLGIKLAKTEGAEY
HHHHHCHHHHHCCCCCHHHHHHHCCCEEEECCHHHCCCCCEEEEHHHCCEEEEECCCCCE
IRLTRDGQAALAFVQRELEN
EEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]