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The map label for this gene is lacG

Identifier: 195977602

GI number: 195977602

Start: 482899

End: 484308

Strand: Direct

Name: lacG

Synonym: Sez_0461

Alternate gene names: 195977602

Gene position: 482899-484308 (Clockwise)

Preceding gene: 195977601

Following gene: 195977603

Centisome position: 23.86

GC content: 43.62

Gene sequence:

>1410_bases
ATGATCAAACGATTACCTGACGATTTCATTTTTGGTGGTGCTACAGCAGCTTATCAGGCTGAGGGTGCCACAACAATAGA
CGGTAAGGGAGCAGTGGCTTGGGATCGCTATTTGAAGGACAATTATTGGTACACAGCAGAGCCAGCGAGTGATTTTTATC
ATCAGTATCCTATTGATCTGGCCTTGGCTGAGCAGTTTGGGATTAATGGTATTCGCATTTCCATTGCTTGGTCGCGGATT
TTCCCAGAGGGCTTTGGCGAGGTTAATGCTAAGGGTGTCGCCTTTTATCACAGCCTTTTTGCTGAATGTCACAAGCACCA
TGTTGAGCCCTTTGTGACCCTGCATCATTTTGACACGCCAGAAGCGCTTCATTCGAGGGGAGATTTTCTCAACCGAGAAA
ACATTGATCACTTTGTTGCTTATGCTGCTTATTGCTTCAAGGAATTTCCAGAGGTGAGCTATTGGACAACCTTTAATGAG
ATTGGACCGATTGGTGATGGACAGTATTTGGTCGGGAAATTTCCTCCTGGCATTAGTTATGACTTGGCCAAGGTATTTCA
ATCACATCACAACATGATGCTAGCTCACGCGCGTGCTCTTGTGCACTACAAGGAACAGGCTTATCCTGGGGAAATTGGGA
TTGTCCATGCTTTGCCAACAAAATACCCTCTAGATCCTACCAATCCAGCAGATGTCCTAGCTGCAGAGCTAGATGATATT
ATTCACAATAAATTTATCCTAGATGCCACTTATCTTGGGCAATACTCAGAAAAAACAATGGCTGGTGTTAAGCATATTCT
TGCTGAAAATGGCGGCAGCCTTGATCTAAGACCAGAAGATTTTGAGCTATTACAGGCAGCCAAGGATTTAAACGATTTTC
TTGGGATTAATTACTACATGAGTGATTGGCTGCGTGCCTTTGATGGTGAGACCGAAATCATTCATAATGGCAAGGGAGAA
AAAGGCGGCTCCAAATATCAGATAAAGGGGGTTGGTCAACGCATATTTGATGTTGATGTGCCGCGTACAGACTGGGATTG
GATGATCTATCCACAGGGGCTTTACGATCAAATCATGCGGATCAAGGCAGACTATCCAGGCTATAAAAAGATTTATATCA
CTGAAAATGGCCTTGGCTACAAGGATACCTGTATTGATGGCAGGATTGATGATGACGCTAGAATTGATTACATCAAGCAG
CATTTGGCGGTGATTGCAGACGTTATCAGTGCTGGGGCTAATGTTAAGGGCTATTTTATCTGGTCCTTGATGGACGTCTT
TTCTTGGTCAAATGGCTATGATAAGCGTTATGGGCTCTTCTATGTTGATTTTGAAACCCAAAAGCGTTACCCTAAAAAAA
GCGCTTATTGGTATCAGCAGCTAGCAGCCACAAAAACCATTTCAGTGTAA

Upstream 100 bases:

>100_bases
GCTAGCCAAGACAGAAGGAGCAGAATACATCAGACTGACGCGTGATGGTCAGGCTGCCTTGGCTTTTGTGCAAAGAGAGC
TAGAAAACTAGGAGGTCATC

Downstream 100 bases:

>100_bases
TCTCCTAACGAAAAGCACCGGCACAAGGGCTGCAAGGCTTCAAGAGCTGGTGCTTTTCTTTTTTATGCTATACTGGTAAC
GATGAGGTCATGACATAAAG

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MIKRLPDDFIFGGATAAYQAEGATTIDGKGAVAWDRYLKDNYWYTAEPASDFYHQYPIDLALAEQFGINGIRISIAWSRI
FPEGFGEVNAKGVAFYHSLFAECHKHHVEPFVTLHHFDTPEALHSRGDFLNRENIDHFVAYAAYCFKEFPEVSYWTTFNE
IGPIGDGQYLVGKFPPGISYDLAKVFQSHHNMMLAHARALVHYKEQAYPGEIGIVHALPTKYPLDPTNPADVLAAELDDI
IHNKFILDATYLGQYSEKTMAGVKHILAENGGSLDLRPEDFELLQAAKDLNDFLGINYYMSDWLRAFDGETEIIHNGKGE
KGGSKYQIKGVGQRIFDVDVPRTDWDWMIYPQGLYDQIMRIKADYPGYKKIYITENGLGYKDTCIDGRIDDDARIDYIKQ
HLAVIADVISAGANVKGYFIWSLMDVFSWSNGYDKRYGLFYVDFETQKRYPKKSAYWYQQLAATKTISV

Sequences:

>Translated_469_residues
MIKRLPDDFIFGGATAAYQAEGATTIDGKGAVAWDRYLKDNYWYTAEPASDFYHQYPIDLALAEQFGINGIRISIAWSRI
FPEGFGEVNAKGVAFYHSLFAECHKHHVEPFVTLHHFDTPEALHSRGDFLNRENIDHFVAYAAYCFKEFPEVSYWTTFNE
IGPIGDGQYLVGKFPPGISYDLAKVFQSHHNMMLAHARALVHYKEQAYPGEIGIVHALPTKYPLDPTNPADVLAAELDDI
IHNKFILDATYLGQYSEKTMAGVKHILAENGGSLDLRPEDFELLQAAKDLNDFLGINYYMSDWLRAFDGETEIIHNGKGE
KGGSKYQIKGVGQRIFDVDVPRTDWDWMIYPQGLYDQIMRIKADYPGYKKIYITENGLGYKDTCIDGRIDDDARIDYIKQ
HLAVIADVISAGANVKGYFIWSLMDVFSWSNGYDKRYGLFYVDFETQKRYPKKSAYWYQQLAATKTISV
>Mature_469_residues
MIKRLPDDFIFGGATAAYQAEGATTIDGKGAVAWDRYLKDNYWYTAEPASDFYHQYPIDLALAEQFGINGIRISIAWSRI
FPEGFGEVNAKGVAFYHSLFAECHKHHVEPFVTLHHFDTPEALHSRGDFLNRENIDHFVAYAAYCFKEFPEVSYWTTFNE
IGPIGDGQYLVGKFPPGISYDLAKVFQSHHNMMLAHARALVHYKEQAYPGEIGIVHALPTKYPLDPTNPADVLAAELDDI
IHNKFILDATYLGQYSEKTMAGVKHILAENGGSLDLRPEDFELLQAAKDLNDFLGINYYMSDWLRAFDGETEIIHNGKGE
KGGSKYQIKGVGQRIFDVDVPRTDWDWMIYPQGLYDQIMRIKADYPGYKKIYITENGLGYKDTCIDGRIDDDARIDYIKQ
HLAVIADVISAGANVKGYFIWSLMDVFSWSNGYDKRYGLFYVDFETQKRYPKKSAYWYQQLAATKTISV

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family

Homologues:

Organism=Homo sapiens, GI110681710, Length=473, Percent_Identity=29.3868921775898, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI13273313, Length=476, Percent_Identity=31.7226890756302, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI32481206, Length=478, Percent_Identity=30.9623430962343, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=27.3662551440329, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI28376633, Length=234, Percent_Identity=31.6239316239316, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789070, Length=494, Percent_Identity=34.0080971659919, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI2367270, Length=494, Percent_Identity=33.1983805668016, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI2367174, Length=492, Percent_Identity=30.0813008130081, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17552856, Length=494, Percent_Identity=31.1740890688259, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17539390, Length=489, Percent_Identity=31.0838445807771, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI21356577, Length=484, Percent_Identity=29.5454545454545, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LACG_STREM (B4U1G6)

Other databases:

- EMBL:   CP001129
- RefSeq:   YP_002122846.1
- EnsemblBacteria:   EBSTRT00000008416
- GeneID:   6761327
- GenomeReviews:   CP001129_GR
- KEGG:   sez:Sez_0461
- GeneTree:   EBGT00050000027409
- HOGENOM:   HBG316462
- OMA:   LYKEKGY
- ProtClustDB:   PRK13511
- HAMAP:   MF_01574
- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928
- Gene3D:   G3DSA:3.20.20.80
- PANTHER:   PTHR10353
- PRINTS:   PR00131
- TIGRFAMs:   TIGR01233

Pfam domain/function: PF00232 Glyco_hydro_1; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.85

Molecular weight: Translated: 53463; Mature: 53463

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: ACT_SITE 160-160 ACT_SITE 375-375

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRLPDDFIFGGATAAYQAEGATTIDGKGAVAWDRYLKDNYWYTAEPASDFYHQYPIDL
CCCCCCCCCEECCCCCEEECCCCEEECCCCCHHHHHHHCCCCEEECCCHHHHHHHCCCCE
ALAEQFGINGIRISIAWSRIFPEGFGEVNAKGVAFYHSLFAECHKHHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EALHSRGDFLNRENIDHFVAYAAYCFKEFPEVSYWTTFNEIGPIGDGQYLVGKFPPGISY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DLAKVFQSHHNMMLAHARALVHYKEQAYPGEIGIVHALPTKYPLDPTNPADVLAAELDDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
IHNKFILDATYLGQYSEKTMAGVKHILAENGGSLDLRPEDFELLQAAKDLNDFLGINYYM
HHCCEEEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
SDWLRAFDGETEIIHNGKGEKGGSKYQIKGVGQRIFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEECCHHHHHHHH
IKADYPGYKKIYITENGLGYKDTCIDGRIDDDARIDYIKQHLAVIADVISAGANVKGYFI
HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHH
WSLMDVFSWSNGYDKRYGLFYVDFETQKRYPKKSAYWYQQLAATKTISV
HHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKRLPDDFIFGGATAAYQAEGATTIDGKGAVAWDRYLKDNYWYTAEPASDFYHQYPIDL
CCCCCCCCCEECCCCCEEECCCCEEECCCCCHHHHHHHCCCCEEECCCHHHHHHHCCCCE
ALAEQFGINGIRISIAWSRIFPEGFGEVNAKGVAFYHSLFAECHKHHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EALHSRGDFLNRENIDHFVAYAAYCFKEFPEVSYWTTFNEIGPIGDGQYLVGKFPPGISY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DLAKVFQSHHNMMLAHARALVHYKEQAYPGEIGIVHALPTKYPLDPTNPADVLAAELDDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
IHNKFILDATYLGQYSEKTMAGVKHILAENGGSLDLRPEDFELLQAAKDLNDFLGINYYM
HHCCEEEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
SDWLRAFDGETEIIHNGKGEKGGSKYQIKGVGQRIFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEECCHHHHHHHH
IKADYPGYKKIYITENGLGYKDTCIDGRIDDDARIDYIKQHLAVIADVISAGANVKGYFI
HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHH
WSLMDVFSWSNGYDKRYGLFYVDFETQKRYPKKSAYWYQQLAATKTISV
HHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA