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The map label for this gene is lacF [H]

Identifier: 195977600

GI number: 195977600

Start: 480892

End: 481203

Strand: Direct

Name: lacF [H]

Synonym: Sez_0459

Alternate gene names: 195977600

Gene position: 480892-481203 (Clockwise)

Preceding gene: 195977599

Following gene: 195977601

Centisome position: 23.76

GC content: 44.87

Gene sequence:

>312_bases
ATGACTAAAGACGAAATAACCATGCTTGGCTTTGAAATTGTTGCTTATGCTGGTGAAGCCAGATCGAAGCTGATTGAGGC
CTTAGAGGCCGCTAAAAAGGGCGATTATGACAAGGCAGAGACGCTTTGTCAATCAGCCAATCAATCCATTTTAGAAGCGC
ATAAGGCGCAGACGAGTCTTTTGCAGGAGGAAGCGACAGGCTCAGATATTGCCTTTAGTGTTACCTTGATGCATGGGCAG
GACCATTTGATGACAACTTTGCTGCTGCAGGATATGATGAAGCACCTTATTGAGCTTTACAAGAGAGGATAA

Upstream 100 bases:

>100_bases
ATCTATCAGCTGATGACAGATTTAACAGGTCAAGAGTTGGTAGAGTCTGAAAAAATCTACATTGTAATCCATATTCAACG
CTTACTATAAAGGAGACAAA

Downstream 100 bases:

>100_bases
GACATGAACAAGCTAATTGAACACATTGAAAAGAGAAAGCCTTTATTTGAGAAGATTTCTCGAAACATTTACCTACGTGC
TATTCGTGATGGCTTTATTG

Product: lactose-specific phosphotransferase enzyme IIA component LacF

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 103; Mature: 102

Protein sequence:

>103_residues
MTKDEITMLGFEIVAYAGEARSKLIEALEAAKKGDYDKAETLCQSANQSILEAHKAQTSLLQEEATGSDIAFSVTLMHGQ
DHLMTTLLLQDMMKHLIELYKRG

Sequences:

>Translated_103_residues
MTKDEITMLGFEIVAYAGEARSKLIEALEAAKKGDYDKAETLCQSANQSILEAHKAQTSLLQEEATGSDIAFSVTLMHGQ
DHLMTTLLLQDMMKHLIELYKRG
>Mature_102_residues
TKDEITMLGFEIVAYAGEARSKLIEALEAAKKGDYDKAETLCQSANQSILEAHKAQTSLLQEEATGSDIAFSVTLMHGQD
HLMTTLLLQDMMKHLIELYKRG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=90, Percent_Identity=35.5555555555556, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 11431; Mature: 11300

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDEITMLGFEIVAYAGEARSKLIEALEAAKKGDYDKAETLCQSANQSILEAHKAQTSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQEEATGSDIAFSVTLMHGQDHLMTTLLLQDMMKHLIELYKRG
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKDEITMLGFEIVAYAGEARSKLIEALEAAKKGDYDKAETLCQSANQSILEAHKAQTSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQEEATGSDIAFSVTLMHGQDHLMTTLLLQDMMKHLIELYKRG
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA