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The map label for this gene is srlE [H]

Identifier: 195977355

GI number: 195977355

Start: 229551

End: 230543

Strand: Direct

Name: srlE [H]

Synonym: Sez_0208

Alternate gene names: 195977355

Gene position: 229551-230543 (Clockwise)

Preceding gene: 195977354

Following gene: 195977356

Centisome position: 11.34

GC content: 45.72

Gene sequence:

>993_bases
ATGATGACTTATCACAGTATTACAGTTGTTAAGGGGAGCGGTGGCTATGGTGGGCCGCTAACCATTACACCAACAGAAGC
TAAGCACAAATTTATCTATATTACTGGCGGTGGAAAAAAGCCTGACATTGTCGATAAAATTGCTGCCCTAACAGGTATGG
AGGCGGTCAATGGCTTTGTTACCTCAATCCCTGATGATGAGATAGCCTTAGCTATTGTGGATTGTGGCGGCACCCTACGC
TGCGGTATTTACCCTAAAAAAGGCATTCCAACCATTAATATCGTGGCGACAGGTAAAAGCGGTCCCTTGGCACAGTACAT
TACCGAGGACATTTACGTGTCTGCTGTAGGTCTTGAGCAAATCACAGCCAGTGACGCTCAAGCACAGACCATCAAGCCTG
AGCCTGTAGCTCCAAAAGCTGCCAGCTATGACACCAACAAGAAAATAACAGAGCAGCGCGCAGAAAACAGCTTTGTGGCT
CGCATTGGCATGGGAGCAGGTAAGGTTGTTGCGATCTTCAACCAAGCGGCGCGTGAGGCCATTCAGACCTTGATCAATAC
GATTCTGCCATTCATGGCATTTGTCTCTCTTTTAATTGGTTTTATTCAAGGTTCAGGTGTTGGAAATTGGCTGGCTAAGC
TCATGGTACCGTTGGCTGGTAATATTTGGGGCTTGATTATCATTGGCTTTATCTGTTCGTTGCCATTTTTATCACCGCTG
TTAGGACCTGGTGCAGTTATTAGCCAAATCATCGGAACGCTCATTGGTGTTGAGATTGGAAAAGGCACTATTCCACCACA
AATGGCTCTGCCTGCGCTTTTTGCCATTAACACGCAAAATGGCTGTGATTTCATTCCAGTAGCACTTGGTTTATCAGAGG
CTAATGCTGAGACAGTAGAGGTTGGTGTGCCATCTGTGCTTTACTCACGCTTTTTAAATGGTGTTCCTCGAGTTGTTGTT
GCTTGGATTGCAAGTATTGGTTTGTATCAGTAA

Upstream 100 bases:

>100_bases
CTTTGTTGGTGGCTGGGTAACTGACTTTACGACAGCCTACGTGGCCAAGCAGCAGGGCGTGACATTAAGCAAGACGATTG
AATTATAGAAGAGAGGTAGC

Downstream 100 bases:

>100_bases
GGAGCTATCGTATGATAGGAAGAGAGAGAATGTATGATAAAAGTATTTGAAACAAGAGTTACCGCTATTGGATCGGAGGC
GGCAGACATGATTAATGCTG

Product: PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components SrlE

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MMTYHSITVVKGSGGYGGPLTITPTEAKHKFIYITGGGKKPDIVDKIAALTGMEAVNGFVTSIPDDEIALAIVDCGGTLR
CGIYPKKGIPTINIVATGKSGPLAQYITEDIYVSAVGLEQITASDAQAQTIKPEPVAPKAASYDTNKKITEQRAENSFVA
RIGMGAGKVVAIFNQAAREAIQTLINTILPFMAFVSLLIGFIQGSGVGNWLAKLMVPLAGNIWGLIIIGFICSLPFLSPL
LGPGAVISQIIGTLIGVEIGKGTIPPQMALPALFAINTQNGCDFIPVALGLSEANAETVEVGVPSVLYSRFLNGVPRVVV
AWIASIGLYQ

Sequences:

>Translated_330_residues
MMTYHSITVVKGSGGYGGPLTITPTEAKHKFIYITGGGKKPDIVDKIAALTGMEAVNGFVTSIPDDEIALAIVDCGGTLR
CGIYPKKGIPTINIVATGKSGPLAQYITEDIYVSAVGLEQITASDAQAQTIKPEPVAPKAASYDTNKKITEQRAENSFVA
RIGMGAGKVVAIFNQAAREAIQTLINTILPFMAFVSLLIGFIQGSGVGNWLAKLMVPLAGNIWGLIIIGFICSLPFLSPL
LGPGAVISQIIGTLIGVEIGKGTIPPQMALPALFAINTQNGCDFIPVALGLSEANAETVEVGVPSVLYSRFLNGVPRVVV
AWIASIGLYQ
>Mature_330_residues
MMTYHSITVVKGSGGYGGPLTITPTEAKHKFIYITGGGKKPDIVDKIAALTGMEAVNGFVTSIPDDEIALAIVDCGGTLR
CGIYPKKGIPTINIVATGKSGPLAQYITEDIYVSAVGLEQITASDAQAQTIKPEPVAPKAASYDTNKKITEQRAENSFVA
RIGMGAGKVVAIFNQAAREAIQTLINTILPFMAFVSLLIGFIQGSGVGNWLAKLMVPLAGNIWGLIIIGFICSLPFLSPL
LGPGAVISQIIGTLIGVEIGKGTIPPQMALPALFAINTQNGCDFIPVALGLSEANAETVEVGVPSVLYSRFLNGVPRVVV
AWIASIGLYQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=331, Percent_Identity=57.7039274924471, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 34500; Mature: 34500

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYHSITVVKGSGGYGGPLTITPTEAKHKFIYITGGGKKPDIVDKIAALTGMEAVNGFV
CCEEEEEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHH
TSIPDDEIALAIVDCGGTLRCGIYPKKGIPTINIVATGKSGPLAQYITEDIYVSAVGLEQ
HCCCCCCEEEEEEECCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEEEECHHH
ITASDAQAQTIKPEPVAPKAASYDTNKKITEQRAENSFVARIGMGAGKVVAIFNQAAREA
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
IQTLINTILPFMAFVSLLIGFIQGSGVGNWLAKLMVPLAGNIWGLIIIGFICSLPFLSPL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LGPGAVISQIIGTLIGVEIGKGTIPPQMALPALFAINTQNGCDFIPVALGLSEANAETVE
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEEEEEEECCCCCCCEEE
VGVPSVLYSRFLNGVPRVVVAWIASIGLYQ
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMTYHSITVVKGSGGYGGPLTITPTEAKHKFIYITGGGKKPDIVDKIAALTGMEAVNGFV
CCEEEEEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHH
TSIPDDEIALAIVDCGGTLRCGIYPKKGIPTINIVATGKSGPLAQYITEDIYVSAVGLEQ
HCCCCCCEEEEEEECCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEEEECHHH
ITASDAQAQTIKPEPVAPKAASYDTNKKITEQRAENSFVARIGMGAGKVVAIFNQAAREA
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
IQTLINTILPFMAFVSLLIGFIQGSGVGNWLAKLMVPLAGNIWGLIIIGFICSLPFLSPL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LGPGAVISQIIGTLIGVEIGKGTIPPQMALPALFAINTQNGCDFIPVALGLSEANAETVE
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEEEEEEECCCCCCCEEE
VGVPSVLYSRFLNGVPRVVVAWIASIGLYQ
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]