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The map label for this gene is srlA [H]

Identifier: 195977354

GI number: 195977354

Start: 228993

End: 229538

Strand: Direct

Name: srlA [H]

Synonym: Sez_0207

Alternate gene names: 195977354

Gene position: 228993-229538 (Clockwise)

Preceding gene: 195977353

Following gene: 195977355

Centisome position: 11.31

GC content: 43.77

Gene sequence:

>546_bases
ATGGAAATGATAACAAGATTTGCGGAAGGCTTTATGAAGCTCTTCCAGCTTGGTGGTGAAACCTTTGTCAGCTGGATGAC
TGGCATTGTGCCGGTGGTCTTGATGCTGCTGGTTGCAATGAATGCCTTAATTGGTATTTTAGGAGATCAAAGGGTCAATC
AATTGGCAAAAATCTCTGCTAAAAACCCTATCAGTCGTTACATGGTATTGCCTTTTATTTCAGCTTTTATGCTGGGTAAT
CCAATGGCAATCAGTATGGGACGTTTTATGCCCGAGTATTACAAGCCAAGCTATGTTGCTGCTCAAATGCAATTTTGTCA
TACGTCAAACGGTGTCTTTCCACACATTAATCCGGGAGAATTGTTTGTCTGGTTAGGTATTGCCTCAGGTATCAAGACGC
TAGGCTTGAGCCAAATGGACTTGGCGATTCGTTACCTCTTGGTTGGGCTAATCATGAACTTTGTTGGTGGCTGGGTAACT
GACTTTACGACAGCCTACGTGGCCAAGCAGCAGGGCGTGACATTAAGCAAGACGATTGAATTATAG

Upstream 100 bases:

>100_bases
TTTTGAGTCCAGCTCAAGCAGCTATTATCCAGCAGGCAGTGTGTCTATATTGCCTTACTTATCTCAATGCTTTTAAGTAC
ATCATATGAGGAGTTTATTT

Downstream 100 bases:

>100_bases
AAGAGAGGTAGCATGATGACTTATCACAGTATTACAGTTGTTAAGGGGAGCGGTGGCTATGGTGGGCCGCTAACCATTAC
ACCAACAGAAGCTAAGCACA

Product: glucitol/sorbitol permease IIC component SrlA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Gut; PTS system glucitol/sorbitol-specific EIIC component [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MEMITRFAEGFMKLFQLGGETFVSWMTGIVPVVLMLLVAMNALIGILGDQRVNQLAKISAKNPISRYMVLPFISAFMLGN
PMAISMGRFMPEYYKPSYVAAQMQFCHTSNGVFPHINPGELFVWLGIASGIKTLGLSQMDLAIRYLLVGLIMNFVGGWVT
DFTTAYVAKQQGVTLSKTIEL

Sequences:

>Translated_181_residues
MEMITRFAEGFMKLFQLGGETFVSWMTGIVPVVLMLLVAMNALIGILGDQRVNQLAKISAKNPISRYMVLPFISAFMLGN
PMAISMGRFMPEYYKPSYVAAQMQFCHTSNGVFPHINPGELFVWLGIASGIKTLGLSQMDLAIRYLLVGLIMNFVGGWVT
DFTTAYVAKQQGVTLSKTIEL
>Mature_181_residues
MEMITRFAEGFMKLFQLGGETFVSWMTGIVPVVLMLLVAMNALIGILGDQRVNQLAKISAKNPISRYMVLPFISAFMLGN
PMAISMGRFMPEYYKPSYVAAQMQFCHTSNGVFPHINPGELFVWLGIASGIKTLGLSQMDLAIRYLLVGLIMNFVGGWVT
DFTTAYVAKQQGVTLSKTIEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3730

COG function: function code G; Phosphotransferase system sorbitol-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994904, Length=181, Percent_Identity=55.8011049723757, Blast_Score=209, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004699 [H]

Pfam domain/function: PF03608 EII-GUT [H]

EC number: 2.7.1.69

Molecular weight: Translated: 20045; Mature: 20045

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS51107 PTS_EIIC_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
7.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMITRFAEGFMKLFQLGGETFVSWMTGIVPVVLMLLVAMNALIGILGDQRVNQLAKISA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
KNPISRYMVLPFISAFMLGNPMAISMGRFMPEYYKPSYVAAQMQFCHTSNGVFPHINPGE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
LFVWLGIASGIKTLGLSQMDLAIRYLLVGLIMNFVGGWVTDFTTAYVAKQQGVTLSKTIE
EEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
L
C
>Mature Secondary Structure
MEMITRFAEGFMKLFQLGGETFVSWMTGIVPVVLMLLVAMNALIGILGDQRVNQLAKISA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
KNPISRYMVLPFISAFMLGNPMAISMGRFMPEYYKPSYVAAQMQFCHTSNGVFPHINPGE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
LFVWLGIASGIKTLGLSQMDLAIRYLLVGLIMNFVGGWVTDFTTAYVAKQQGVTLSKTIE
EEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]