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The map label for this gene is purD [H]

Identifier: 195977186

GI number: 195977186

Start: 42571

End: 43875

Strand: Direct

Name: purD [H]

Synonym: Sez_0030

Alternate gene names: 195977186

Gene position: 42571-43875 (Clockwise)

Preceding gene: 195977184

Following gene: 195977187

Centisome position: 2.1

GC content: 46.44

Gene sequence:

>1305_bases
ATGCTAAAATATGTTTATAAAATTCGTGTTATGAGGTGTGTGATGAAGCTTTTAGTTATCGGTTCAGGTGGTCGTGAACA
TGCGATTGCTAAGAAATTGTTAGAGTCAAAAGGCGTTGATCAGGTTTTTGTGGCACCTGGCAATGATGGCATGACCCTTG
ATGGCATAGAACGGGTAGATATTGATATTTCCGAACATTCTGCTTTGATTGCTTTTGCAAAGACCAATCATATAGCCTGG
ACCTTTGTGGGACCAGATGATGCGTTAGCGGCTGGTATTGTTGATGAGTTTAATCAGGCTGGCTTGAAAGCCTTTGGGCC
ATCTCGTTTAGCAGCGGAGCTGGAGTGGTCAAAGGATTTTGCTAAGCAAATCATGGTCAAATACGGTATTCCAACAGCAG
CCTATGACACATTTTCTGATTTTGCAAAAGCCAAGGCCTACATCGAGGAAAAGGGCGTGCCAATTGTGGTTAAGGCAGAT
GGTTTGGCGCTAGGAAAGGGTGTTGTCGTAGCAGAGACTGTAGAGCAGGCGGTTGAAGCGGCGCGTGACATGCTCTTGGA
TAATAAATTTGGTGATTCAGGTGCACGCGTGGTGATTGAGGAGTTTTTAACTGGTGAAGAATTCTCTCTGTTTGCCTTTG
TGAATGGTGATCAGTTTTACCTCATGCCGACAGCGCAGGACCATAAACGGGCTCTTGATGGTGACAAGGGGCCAAATACA
GGTGGTATGGGAGCCTATGCGCCAGTTCCTCATCTCCCTCAAAGTGTGGTGGATACAGCTGTTGAGACTATCGTGAAGCC
AGTCCTCAAGGGCATGATGGCAGAGGGGCGTCCTTACCTTGGGATTCTCTACTGTGGCTTAATTTTGACAGAAGATGGAC
CAAAAGTGATTGAGTTCAATGCCCGCTTTGGTGATCCTGAGGCTCAGGTTATCCTTCCTCGCTTGACCTCTGATTTTGCG
CAGAACATTACTGATATGTTAGAGGGCAACACAGCTAATATCACATGGTCAGACGAGGGAGTGACGCTCGGCGTTGTCCT
TGCTTCAAACGGTTATCCGCTTGCTTACAAGAAAGGTGTGCTTCTGCCAGCAAAGACCACTGGCGACATCATTACCTACT
ACGCAGGCGTTAAGCTTGCGGAAAATGGCAAAGCACTGCTGTCAAATGGTGGACGTGTCTATATGCTGGTCACCACAGCA
GTTACCGTCCCAGCTGCGCAAGAAAAAATCTATACCGAACTTGCTCAGCAAAATACCGAAGGATTATTCTACCGAAACGA
TATTGGAAGAAAAGCAGTAAAATAA

Upstream 100 bases:

>100_bases
TTTGGTGGTTTATTCTATAAACCTTATTTTTAAAAAAGGTATCATTTTTTTATGTTTTGAGCTTTCTTTGGTGATTAGTT
ATTTATATAAACGAACAATT

Downstream 100 bases:

>100_bases
ATTAGCGCTAGCTCAAGCTACCGAAGGTCGCTATGATGACAATGACTTATTATCGGTGAGCAAAAGCGAGCCTAACACGA
TACTTTTCGCCGTAGCGCAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLKYVYKIRVMRCVMKLLVIGSGGREHAIAKKLLESKGVDQVFVAPGNDGMTLDGIERVDIDISEHSALIAFAKTNHIAW
TFVGPDDALAAGIVDEFNQAGLKAFGPSRLAAELEWSKDFAKQIMVKYGIPTAAYDTFSDFAKAKAYIEEKGVPIVVKAD
GLALGKGVVVAETVEQAVEAARDMLLDNKFGDSGARVVIEEFLTGEEFSLFAFVNGDQFYLMPTAQDHKRALDGDKGPNT
GGMGAYAPVPHLPQSVVDTAVETIVKPVLKGMMAEGRPYLGILYCGLILTEDGPKVIEFNARFGDPEAQVILPRLTSDFA
QNITDMLEGNTANITWSDEGVTLGVVLASNGYPLAYKKGVLLPAKTTGDIITYYAGVKLAENGKALLSNGGRVYMLVTTA
VTVPAAQEKIYTELAQQNTEGLFYRNDIGRKAVK

Sequences:

>Translated_434_residues
MLKYVYKIRVMRCVMKLLVIGSGGREHAIAKKLLESKGVDQVFVAPGNDGMTLDGIERVDIDISEHSALIAFAKTNHIAW
TFVGPDDALAAGIVDEFNQAGLKAFGPSRLAAELEWSKDFAKQIMVKYGIPTAAYDTFSDFAKAKAYIEEKGVPIVVKAD
GLALGKGVVVAETVEQAVEAARDMLLDNKFGDSGARVVIEEFLTGEEFSLFAFVNGDQFYLMPTAQDHKRALDGDKGPNT
GGMGAYAPVPHLPQSVVDTAVETIVKPVLKGMMAEGRPYLGILYCGLILTEDGPKVIEFNARFGDPEAQVILPRLTSDFA
QNITDMLEGNTANITWSDEGVTLGVVLASNGYPLAYKKGVLLPAKTTGDIITYYAGVKLAENGKALLSNGGRVYMLVTTA
VTVPAAQEKIYTELAQQNTEGLFYRNDIGRKAVK
>Mature_434_residues
MLKYVYKIRVMRCVMKLLVIGSGGREHAIAKKLLESKGVDQVFVAPGNDGMTLDGIERVDIDISEHSALIAFAKTNHIAW
TFVGPDDALAAGIVDEFNQAGLKAFGPSRLAAELEWSKDFAKQIMVKYGIPTAAYDTFSDFAKAKAYIEEKGVPIVVKAD
GLALGKGVVVAETVEQAVEAARDMLLDNKFGDSGARVVIEEFLTGEEFSLFAFVNGDQFYLMPTAQDHKRALDGDKGPNT
GGMGAYAPVPHLPQSVVDTAVETIVKPVLKGMMAEGRPYLGILYCGLILTEDGPKVIEFNARFGDPEAQVILPRLTSDFA
QNITDMLEGNTANITWSDEGVTLGVVLASNGYPLAYKKGVLLPAKTTGDIITYYAGVKLAENGKALLSNGGRVYMLVTTA
VTVPAAQEKIYTELAQQNTEGLFYRNDIGRKAVK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=45.1764705882353, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=45.1764705882353, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=45.1764705882353, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=45.1764705882353, Blast_Score=350, Evalue=1e-96,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=47.3067915690867, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17567511, Length=429, Percent_Identity=39.3939393939394, Blast_Score=270, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=38.7755102040816, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=42.5925925925926, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=42.5925925925926, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46816; Mature: 46816

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKYVYKIRVMRCVMKLLVIGSGGREHAIAKKLLESKGVDQVFVAPGNDGMTLDGIERVD
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCEEEE
IDISEHSALIAFAKTNHIAWTFVGPDDALAAGIVDEFNQAGLKAFGPSRLAAELEWSKDF
EEECCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AKQIMVKYGIPTAAYDTFSDFAKAKAYIEEKGVPIVVKADGLALGKGVVVAETVEQAVEA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHH
ARDMLLDNKFGDSGARVVIEEFLTGEEFSLFAFVNGDQFYLMPTAQDHKRALDGDKGPNT
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCC
GGMGAYAPVPHLPQSVVDTAVETIVKPVLKGMMAEGRPYLGILYCGLILTEDGPKVIEFN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEE
ARFGDPEAQVILPRLTSDFAQNITDMLEGNTANITWSDEGVTLGVVLASNGYPLAYKKGV
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCEEEECCE
LLPAKTTGDIITYYAGVKLAENGKALLSNGGRVYMLVTTAVTVPAAQEKIYTELAQQNTE
EEECCCCCCEEEEEECEEEECCCCHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHCCCC
GLFYRNDIGRKAVK
CEEEECCCCHHCCC
>Mature Secondary Structure
MLKYVYKIRVMRCVMKLLVIGSGGREHAIAKKLLESKGVDQVFVAPGNDGMTLDGIERVD
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCEEEE
IDISEHSALIAFAKTNHIAWTFVGPDDALAAGIVDEFNQAGLKAFGPSRLAAELEWSKDF
EEECCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AKQIMVKYGIPTAAYDTFSDFAKAKAYIEEKGVPIVVKADGLALGKGVVVAETVEQAVEA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHH
ARDMLLDNKFGDSGARVVIEEFLTGEEFSLFAFVNGDQFYLMPTAQDHKRALDGDKGPNT
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCC
GGMGAYAPVPHLPQSVVDTAVETIVKPVLKGMMAEGRPYLGILYCGLILTEDGPKVIEFN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEE
ARFGDPEAQVILPRLTSDFAQNITDMLEGNTANITWSDEGVTLGVVLASNGYPLAYKKGV
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCEEEECCE
LLPAKTTGDIITYYAGVKLAENGKALLSNGGRVYMLVTTAVTVPAAQEKIYTELAQQNTE
EEECCCCCCEEEEEECEEEECCCCHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHCCCC
GLFYRNDIGRKAVK
CEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]