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The map label for this gene is dnaN [H]

Identifier: 195977158

GI number: 195977158

Start: 1742

End: 2878

Strand: Direct

Name: dnaN [H]

Synonym: Sez_0002

Alternate gene names: 195977158

Gene position: 1742-2878 (Clockwise)

Preceding gene: 195977157

Following gene: 195977159

Centisome position: 0.09

GC content: 38.43

Gene sequence:

>1137_bases
ATGATTCAATTTTCTATCAATAGAGCCCTTTTCATTCAAGCTTTAACAGCCACTAAAAGGGCTATCAGCAGTAAAAATGC
TATACCGATCTTATCAACCATCAAGATTGAGGTTAATCCATCAGACATTACCCTGACTGGCTCTAATGGTCAAATCTCTA
TTGAAAATACCATTCCTGTTAGCAATGAAAACGCAGGCTTGTTGATAACCTCAACAGGGGCTGTTCTTTTAGAAGCCAGC
TTTTTTATCAATATTATCTCAAGTCTTCCAGATGTTAGCTTAGAATTTAAGGAAATTGAACAGCATCAGGTGGTGCTAAC
CAGTGGGAAATCTGAAATCACCTTAAAGGGGAAAGATGTTTCTCAATACCCACGTCTTCAAGAGGTATCAACTGAAAATC
CTTTAATTTTAAATACAAGACTCTTAAAATCTATCATTGCTGAGACAGCCTTTGCTGCTAGTATGCAGGAAAGTCGTCCT
ATTTTGACAGGTGTACACATTACCTTAAGTCAACATAAGGACTTCAAAGCGGTAGCAACAGATTCACATCGTATGAGTCA
ACGTTTGATTACTTTGGAGAAAACCTCAGCTGATTTTGATGTGGTTATTCCAAGTAAATCCTTGAGAGAATTTTCAGCTG
TCTTTACAGATGATATTGAAACCGTTGAAGTGTTCTTTTCTGCTAGTCAAATGCTATTTAGAAGTGACTACATTTCTTTT
TACACCCGTCTGTTAGAAGGTAATTACCCTGATACTGATCGTTTGCTGATGAAGCAGTTTGAGACAGAGGCTGTTTTTAA
CACACAATCCTTACGACATGCTATGGAGCGTGCTTTCTTGATTTCAAACGCTACGCAAAATGGTACTGTTAAGCTTGAAA
TTGAAGCAAATCGCATTTCAGCTCATGTTAACTCTCCAGAGGTTGGTAAGGTTAACGAGGATCTGGACGTGGTGAGTCAA
GCAGGGAATGATTTAACCATTAGCTTTAACCCAACCTATCTGATTGAAGCCTTAAAAGCCATTAAAAGTGAGACTGTTAA
GATTCACTTCCTATCGCCTGTTCGTCCTTTCACCTTAACACCGGGAGATGATGAAGAGAGCTTTATCCAATTAATCACAC
CAGTTCGTACAAACTAA

Upstream 100 bases:

>100_bases
GTTTTAACTAGTAACGACTAGTACAAGGAGACTTATCCACTATTTTAACAAGACCTACTACTACTACTAGCTATAATACT
TATATAACAAAGGAGTTACC

Downstream 100 bases:

>100_bases
GCGAAAAATGCTCCCTTTTAGGAGCTTTTTTTGTTATGATAAATAATAAATATAAGAGATGAGTGAGTGATAAAGTGATG
TATCAAGTTGGATCCTTAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIQFSINRALFIQALTATKRAISSKNAIPILSTIKIEVNPSDITLTGSNGQISIENTIPVSNENAGLLITSTGAVLLEAS
FFINIISSLPDVSLEFKEIEQHQVVLTSGKSEITLKGKDVSQYPRLQEVSTENPLILNTRLLKSIIAETAFAASMQESRP
ILTGVHITLSQHKDFKAVATDSHRMSQRLITLEKTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSASQMLFRSDYISF
YTRLLEGNYPDTDRLLMKQFETEAVFNTQSLRHAMERAFLISNATQNGTVKLEIEANRISAHVNSPEVGKVNEDLDVVSQ
AGNDLTISFNPTYLIEALKAIKSETVKIHFLSPVRPFTLTPGDDEESFIQLITPVRTN

Sequences:

>Translated_378_residues
MIQFSINRALFIQALTATKRAISSKNAIPILSTIKIEVNPSDITLTGSNGQISIENTIPVSNENAGLLITSTGAVLLEAS
FFINIISSLPDVSLEFKEIEQHQVVLTSGKSEITLKGKDVSQYPRLQEVSTENPLILNTRLLKSIIAETAFAASMQESRP
ILTGVHITLSQHKDFKAVATDSHRMSQRLITLEKTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSASQMLFRSDYISF
YTRLLEGNYPDTDRLLMKQFETEAVFNTQSLRHAMERAFLISNATQNGTVKLEIEANRISAHVNSPEVGKVNEDLDVVSQ
AGNDLTISFNPTYLIEALKAIKSETVKIHFLSPVRPFTLTPGDDEESFIQLITPVRTN
>Mature_378_residues
MIQFSINRALFIQALTATKRAISSKNAIPILSTIKIEVNPSDITLTGSNGQISIENTIPVSNENAGLLITSTGAVLLEAS
FFINIISSLPDVSLEFKEIEQHQVVLTSGKSEITLKGKDVSQYPRLQEVSTENPLILNTRLLKSIIAETAFAASMQESRP
ILTGVHITLSQHKDFKAVATDSHRMSQRLITLEKTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSASQMLFRSDYISF
YTRLLEGNYPDTDRLLMKQFETEAVFNTQSLRHAMERAFLISNATQNGTVKLEIEANRISAHVNSPEVGKVNEDLDVVSQ
AGNDLTISFNPTYLIEALKAIKSETVKIHFLSPVRPFTLTPGDDEESFIQLITPVRTN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=347, Percent_Identity=23.0547550432277, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41930; Mature: 41930

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFSINRALFIQALTATKRAISSKNAIPILSTIKIEVNPSDITLTGSNGQISIENTIPV
CEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEECCCCEEEEECCCCC
SNENAGLLITSTGAVLLEASFFINIISSLPDVSLEFKEIEQHQVVLTSGKSEITLKGKDV
CCCCCCEEEEECCCEEEHHHHHHHHHHHCCCCEEEHHHCCCEEEEEECCCCEEEEECCCH
SQYPRLQEVSTENPLILNTRLLKSIIAETAFAASMQESRPILTGVHITLSQHKDFKAVAT
HHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEC
DSHRMSQRLITLEKTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSASQMLFRSDYISF
CCHHHHHHEEEEEECCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YTRLLEGNYPDTDRLLMKQFETEAVFNTQSLRHAMERAFLISNATQNGTVKLEIEANRIS
HHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHEEECCCCCCEEEEEEEEEEEE
AHVNSPEVGKVNEDLDVVSQAGNDLTISFNPTYLIEALKAIKSETVKIHFLSPVRPFTLT
EECCCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCEEEC
PGDDEESFIQLITPVRTN
CCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIQFSINRALFIQALTATKRAISSKNAIPILSTIKIEVNPSDITLTGSNGQISIENTIPV
CEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEECCCCEEEEECCCCC
SNENAGLLITSTGAVLLEASFFINIISSLPDVSLEFKEIEQHQVVLTSGKSEITLKGKDV
CCCCCCEEEEECCCEEEHHHHHHHHHHHCCCCEEEHHHCCCEEEEEECCCCEEEEECCCH
SQYPRLQEVSTENPLILNTRLLKSIIAETAFAASMQESRPILTGVHITLSQHKDFKAVAT
HHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEC
DSHRMSQRLITLEKTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSASQMLFRSDYISF
CCHHHHHHEEEEEECCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YTRLLEGNYPDTDRLLMKQFETEAVFNTQSLRHAMERAFLISNATQNGTVKLEIEANRIS
HHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHEEECCCCCCEEEEEEEEEEEE
AHVNSPEVGKVNEDLDVVSQAGNDLTISFNPTYLIEALKAIKSETVKIHFLSPVRPFTLT
EECCCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCEEEC
PGDDEESFIQLITPVRTN
CCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]