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The map label for this gene is pyrE [H]

Identifier: 195952820

GI number: 195952820

Start: 386173

End: 386727

Strand: Direct

Name: pyrE [H]

Synonym: HY04AAS1_0442

Alternate gene names: 195952820

Gene position: 386173-386727 (Clockwise)

Preceding gene: 195952819

Following gene: 195952821

Centisome position: 24.76

GC content: 33.87

Gene sequence:

>555_bases
ATGCAAAATCAAGATTATCTTCTTAAGTTAAAAGAACTTATAAAAAAATATTCTCTTTTGGTAGCAGATGAACCTAAGTT
TAAACTAGTTTCTGGTAAACTTAGCAGGTACTATATAGATTTAAAACAAGTTACCTTTGATCCAGAAGGCATATACATAC
TTGGCAACGTTTTGTATGATATGTTAAAACCTTTTGACGTTGATGCTGCAGGTGGGCTTACGCTAGGAGCCGATCCTATG
GCATATGCGGTTTCAATTATATCCTTTGAAAAAGGTAACCCTATAAAACCTTTTGTAGTAAGAAAAGAGCCAAAAGATCA
CGGCATGGGTAAGCAAATAGAAGGTAAGCTAGAAAACGTTAAAAATGTGGCTATATTAGAAGATGTATCTACTACAGGTG
GGTCTTCTTTAAAAGCTGCAAACGTTTGCAAAAACGCTTCTTTGAACGTGGTAGGTATATTTACCATCGTAGATAGAGAA
GAAGGCGCCAAAGAAAATATAGAAAAGGAAGGTTTTAAATTTTATTCTGTGTTTAAATTATTTGAGCTTTTGTGA

Upstream 100 bases:

>100_bases
AATGGTATAGTAAAAAATGTTACAAAAAACAAAACCTATTCTTCTACTAAATTTCCAGAAGAGCTTATGGCTATACTAAG
AGCTGGTGGGTTGATGGAAT

Downstream 100 bases:

>100_bases
AAAAAAGACTAGATCTATTGTTGGTGGAAAAGGGCTTGGTTTCTTCTCGTGAGAAAGCTCAAGCTTTTATAATGGAGGGA
AGGGTTTTTGTAAATGGTAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MQNQDYLLKLKELIKKYSLLVADEPKFKLVSGKLSRYYIDLKQVTFDPEGIYILGNVLYDMLKPFDVDAAGGLTLGADPM
AYAVSIISFEKGNPIKPFVVRKEPKDHGMGKQIEGKLENVKNVAILEDVSTTGGSSLKAANVCKNASLNVVGIFTIVDRE
EGAKENIEKEGFKFYSVFKLFELL

Sequences:

>Translated_184_residues
MQNQDYLLKLKELIKKYSLLVADEPKFKLVSGKLSRYYIDLKQVTFDPEGIYILGNVLYDMLKPFDVDAAGGLTLGADPM
AYAVSIISFEKGNPIKPFVVRKEPKDHGMGKQIEGKLENVKNVAILEDVSTTGGSSLKAANVCKNASLNVVGIFTIVDRE
EGAKENIEKEGFKFYSVFKLFELL
>Mature_184_residues
MQNQDYLLKLKELIKKYSLLVADEPKFKLVSGKLSRYYIDLKQVTFDPEGIYILGNVLYDMLKPFDVDAAGGLTLGADPM
AYAVSIISFEKGNPIKPFVVRKEPKDHGMGKQIEGKLENVKNVAILEDVSTTGGSSLKAANVCKNASLNVVGIFTIVDRE
EGAKENIEKEGFKFYSVFKLFELL

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=160, Percent_Identity=29.375, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=161, Percent_Identity=31.055900621118, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=161, Percent_Identity=27.3291925465839, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20476; Mature: 20476

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQDYLLKLKELIKKYSLLVADEPKFKLVSGKLSRYYIDLKQVTFDPEGIYILGNVLYD
CCCHHHHHHHHHHHHHHHEEEECCCCEEEECCCCEEEEEEEEHEEECCCCEEEEHHHHHH
MLKPFDVDAAGGLTLGADPMAYAVSIISFEKGNPIKPFVVRKEPKDHGMGKQIEGKLENV
HHCCCCCCCCCCEEECCCHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHCC
KNVAILEDVSTTGGSSLKAANVCKNASLNVVGIFTIVDREEGAKENIEKEGFKFYSVFKL
CCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHCHHHHHHHHH
FELL
HHHC
>Mature Secondary Structure
MQNQDYLLKLKELIKKYSLLVADEPKFKLVSGKLSRYYIDLKQVTFDPEGIYILGNVLYD
CCCHHHHHHHHHHHHHHHEEEECCCCEEEECCCCEEEEEEEEHEEECCCCEEEEHHHHHH
MLKPFDVDAAGGLTLGADPMAYAVSIISFEKGNPIKPFVVRKEPKDHGMGKQIEGKLENV
HHCCCCCCCCCCEEECCCHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHCC
KNVAILEDVSTTGGSSLKAANVCKNASLNVVGIFTIVDREEGAKENIEKEGFKFYSVFKL
CCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHCHHHHHHHHH
FELL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]