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The map label for this gene is purH [H]

Identifier: 195952470

GI number: 195952470

Start: 84037

End: 85557

Strand: Direct

Name: purH [H]

Synonym: HY04AAS1_0090

Alternate gene names: 195952470

Gene position: 84037-85557 (Clockwise)

Preceding gene: 195952469

Following gene: 195952471

Centisome position: 5.39

GC content: 36.62

Gene sequence:

>1521_bases
ATGAGAGCACTTATATCTGTATACGATAAAACCGGTATTTTAGAACTGGCTAAGGAGCTTTTGAACCAAGGGTATGAGAT
TCTATCCAGTGGTGGGACATACACTTATCTAAAAAATGCTGGTGTTGATGCCATAGAGGTATCTGAGGTAACAGGTTTTA
GAGAGATTTTAGGTGGTAGAGTTAAGACGCTTCATCCCGCTATACACGGTGGTATTTTGTTTAGAGAAGACGTAGAAAAA
GATTTAGAAGAAATAAAAGAAAACTCTATAGAACCTATTGATATCGTAGTGGTGAACTTGTATCCTTTTGAAAAGAAGAT
GAAAGAGCTAAAAGATATAGATGCTCTTGTGGAGTTTATAGACATAGGGGGTCCTACTCTTGTAAGAGCCGCTGCTAAAA
ATCACAAACGAGTTAGTGTACTTACAGATATCGAAGATTACGGATGGTTTATAGAAAAGCTCAAAATGAATGCTGTATCT
CAGCAAGACAGAAAATACTTAGCTTTGAAAGCTTTTTGGTTAACATCTTACTATGATGCTGTTATAGCTAGTTATTTTTC
CAAAGTATTTGGCTTTTCAGAAAAAGATTTTAAGCATCATACTGTACCTATGTTTTTGAGAGATGAATTGAGATACGGTG
AAAATCCACATCAGCAAGCTTACCTATATGAAAATCCGTTGGAAGAAAATGGTATTGTAAGAGCTGATGTGCTTCAAGGT
AAAAAGATGTCTTACAACAACTATCTTGATGCCGATTCTGTTGTAAAGCTCATGTCAGAGTTTTCTAATCCTTGCTGTGC
TATCGTAAAACACAACAATCCAAGCGGTATAACCACAGACAACAATATTCTGGAAGCTTACAAAAAAGCTTTTCAATGTG
ATCCTGAGGCGGCGTTTGGTGGTATCGTAGCTTTTAACAAGGTTGTAGATAAAGACGTTGCTAAGGCTATTACAGAGCAT
TTTTATGAGATAGTTATAGCTCCTGAGTTTACCGAAGAAGCTGTTGAAGAGTTTTCCAAAAAGAAGAATTTAAGGTTAGT
AAGGTATAAAAACTACAATCAGAATATTGATCTAAGGAGTATATCTGGCGGGTTTTTAGTGCAGGACATAGATGATAAGC
TTTATGAGTCTATAGAGATAGTTTCATTGAGAAGACCTACTGAGCAAGAGTTAGAGGATGCTATATTTGCTTGGAAAGTG
GCAAAATGGACAAAATCAAATGCTATAGTGATAGCTAAAAACAATCAAACCATAGGTATAGGCGCTGGTCAGGTGTCGAG
GGTAGATTCTCTTAGAAGCGCTATAAGAAAAGCAAAAAACTTTTCCCATGATTTAAAAGGCGCCGTAGTGGCCTCAGACG
CTTTTTTCCCGTTTAGAGATAGCATAGATATAGCTGCTGAAGAAGGAATATCTGGTACAATACAACCTGGTGGTTCTATA
AGAGACAAAGAAGTTATAGAGGCTGTAAATGAGCATAACATGTTTATGATATTTACCCATATGAGGCATTTCAGACATTG
A

Upstream 100 bases:

>100_bases
TAAATTACCAAAGACAAGAAGTGGAAAGATTATGAGAAGAGTACTAAAAGCCAAAGAGCTTGGGCTTGATATAGGCGATG
TATCTACCTTAGAGGATTAA

Downstream 100 bases:

>100_bases
AGCTTTGCAAATTTGAGAATTGTTTTTATATTTTATGTATATCCAATAGTGAGAGGTTTTTATGAAATTACCACCACTTA
GAATAAGGCATATAGAGGTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRALISVYDKTGILELAKELLNQGYEILSSGGTYTYLKNAGVDAIEVSEVTGFREILGGRVKTLHPAIHGGILFREDVEK
DLEEIKENSIEPIDIVVVNLYPFEKKMKELKDIDALVEFIDIGGPTLVRAAAKNHKRVSVLTDIEDYGWFIEKLKMNAVS
QQDRKYLALKAFWLTSYYDAVIASYFSKVFGFSEKDFKHHTVPMFLRDELRYGENPHQQAYLYENPLEENGIVRADVLQG
KKMSYNNYLDADSVVKLMSEFSNPCCAIVKHNNPSGITTDNNILEAYKKAFQCDPEAAFGGIVAFNKVVDKDVAKAITEH
FYEIVIAPEFTEEAVEEFSKKKNLRLVRYKNYNQNIDLRSISGGFLVQDIDDKLYESIEIVSLRRPTEQELEDAIFAWKV
AKWTKSNAIVIAKNNQTIGIGAGQVSRVDSLRSAIRKAKNFSHDLKGAVVASDAFFPFRDSIDIAAEEGISGTIQPGGSI
RDKEVIEAVNEHNMFMIFTHMRHFRH

Sequences:

>Translated_506_residues
MRALISVYDKTGILELAKELLNQGYEILSSGGTYTYLKNAGVDAIEVSEVTGFREILGGRVKTLHPAIHGGILFREDVEK
DLEEIKENSIEPIDIVVVNLYPFEKKMKELKDIDALVEFIDIGGPTLVRAAAKNHKRVSVLTDIEDYGWFIEKLKMNAVS
QQDRKYLALKAFWLTSYYDAVIASYFSKVFGFSEKDFKHHTVPMFLRDELRYGENPHQQAYLYENPLEENGIVRADVLQG
KKMSYNNYLDADSVVKLMSEFSNPCCAIVKHNNPSGITTDNNILEAYKKAFQCDPEAAFGGIVAFNKVVDKDVAKAITEH
FYEIVIAPEFTEEAVEEFSKKKNLRLVRYKNYNQNIDLRSISGGFLVQDIDDKLYESIEIVSLRRPTEQELEDAIFAWKV
AKWTKSNAIVIAKNNQTIGIGAGQVSRVDSLRSAIRKAKNFSHDLKGAVVASDAFFPFRDSIDIAAEEGISGTIQPGGSI
RDKEVIEAVNEHNMFMIFTHMRHFRH
>Mature_506_residues
MRALISVYDKTGILELAKELLNQGYEILSSGGTYTYLKNAGVDAIEVSEVTGFREILGGRVKTLHPAIHGGILFREDVEK
DLEEIKENSIEPIDIVVVNLYPFEKKMKELKDIDALVEFIDIGGPTLVRAAAKNHKRVSVLTDIEDYGWFIEKLKMNAVS
QQDRKYLALKAFWLTSYYDAVIASYFSKVFGFSEKDFKHHTVPMFLRDELRYGENPHQQAYLYENPLEENGIVRADVLQG
KKMSYNNYLDADSVVKLMSEFSNPCCAIVKHNNPSGITTDNNILEAYKKAFQCDPEAAFGGIVAFNKVVDKDVAKAITEH
FYEIVIAPEFTEEAVEEFSKKKNLRLVRYKNYNQNIDLRSISGGFLVQDIDDKLYESIEIVSLRRPTEQELEDAIFAWKV
AKWTKSNAIVIAKNNQTIGIGAGQVSRVDSLRSAIRKAKNFSHDLKGAVVASDAFFPFRDSIDIAAEEGISGTIQPGGSI
RDKEVIEAVNEHNMFMIFTHMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=36.7983367983368, Blast_Score=237, Evalue=2e-62,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=47.6099426386233, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71985564, Length=485, Percent_Identity=35.8762886597938, Blast_Score=238, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=316, Percent_Identity=28.4810126582279, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=48.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=38.3157894736842, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=475, Percent_Identity=38.1052631578947, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24649832, Length=474, Percent_Identity=35.6540084388186, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57283; Mature: 57283

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALISVYDKTGILELAKELLNQGYEILSSGGTYTYLKNAGVDAIEVSEVTGFREILGGR
CCCHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHCCC
VKTLHPAIHGGILFREDVEKDLEEIKENSIEPIDIVVVNLYPFEKKMKELKDIDALVEFI
HHEECHHHHCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
DIGGPTLVRAAAKNHKRVSVLTDIEDYGWFIEKLKMNAVSQQDRKYLALKAFWLTSYYDA
HCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIASYFSKVFGFSEKDFKHHTVPMFLRDELRYGENPHQQAYLYENPLEENGIVRADVLQG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEHHCC
KKMSYNNYLDADSVVKLMSEFSNPCCAIVKHNNPSGITTDNNILEAYKKAFQCDPEAAFG
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
GIVAFNKVVDKDVAKAITEHFYEIVIAPEFTEEAVEEFSKKKNLRLVRYKNYNQNIDLRS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
ISGGFLVQDIDDKLYESIEIVSLRRPTEQELEDAIFAWKVAKWTKSNAIVIAKNNQTIGI
CCCCEEEEHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEE
GAGQVSRVDSLRSAIRKAKNFSHDLKGAVVASDAFFPFRDSIDIAAEEGISGTIQPGGSI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDKEVIEAVNEHNMFMIFTHMRHFRH
CHHHHHHHHCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MRALISVYDKTGILELAKELLNQGYEILSSGGTYTYLKNAGVDAIEVSEVTGFREILGGR
CCCHHHHHCCCCHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHCCC
VKTLHPAIHGGILFREDVEKDLEEIKENSIEPIDIVVVNLYPFEKKMKELKDIDALVEFI
HHEECHHHHCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
DIGGPTLVRAAAKNHKRVSVLTDIEDYGWFIEKLKMNAVSQQDRKYLALKAFWLTSYYDA
HCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIASYFSKVFGFSEKDFKHHTVPMFLRDELRYGENPHQQAYLYENPLEENGIVRADVLQG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEHHCC
KKMSYNNYLDADSVVKLMSEFSNPCCAIVKHNNPSGITTDNNILEAYKKAFQCDPEAAFG
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
GIVAFNKVVDKDVAKAITEHFYEIVIAPEFTEEAVEEFSKKKNLRLVRYKNYNQNIDLRS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
ISGGFLVQDIDDKLYESIEIVSLRRPTEQELEDAIFAWKVAKWTKSNAIVIAKNNQTIGI
CCCCEEEEHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEE
GAGQVSRVDSLRSAIRKAKNFSHDLKGAVVASDAFFPFRDSIDIAAEEGISGTIQPGGSI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDKEVIEAVNEHNMFMIFTHMRHFRH
CHHHHHHHHCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]