Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 194738017

GI number: 194738017

Start: 2660911

End: 2662077

Strand: Reverse

Name: rlmN [H]

Synonym: SeSA_A2764

Alternate gene names: 194738017

Gene position: 2662077-2660911 (Counterclockwise)

Preceding gene: 194736317

Following gene: 194737551

Centisome position: 56.53

GC content: 52.1

Gene sequence:

>1167_bases
ATGTCTGAACAAATTGTCACACCTGAAAGCAGTACCCCGGTAGTTCTTAACAACGAAACGAAAATCAACCTGCTGGATCT
CAACCGTCAGCAGATGCGCGAGTTTTTTAAAAACTTAGGTGAAAAGCCTTTCCGCGCCGATCAGGTGATGAAATGGATGT
ATCACTATTGCTGCGATAATTTTGATGAGATGACCGACATCAACAAAGTGTTGCGCGGTAAATTAAAAGAGGTCGCGGAA
ATCCGCGCGCCGGAGGTCGTTGAAGAGCAGCGTTCCTCAGACGGCACCATTAAGTGGGCGATTGCTGTCGGCGATCAGCG
CGTTGAAACGGTCTATATCCCGGAAGACGACCGCGCTACGCTGTGTGTCTCTTCACAGGTAGGATGCGCGCTGGAGTGCA
AGTTTTGTTCAACTGCGCAGCAGGGCTTTAACCGCAACCTGCGTGTATCGGAAATTATCGGTCAGGTCTGGCGTGCGGCG
AAAATCGTCGGCGCGGCCAAAGTCACCGGCCAGCGTCCGATCACCAACGTAGTCATGATGGGAATGGGCGAGCCATTGCT
TAACCTGACCAATGTCGTACCGGCGATGGAAATTATGCTCGATGACTTTGGTTTCGGCCTGTCCAAACGTCGTGTCACGC
TCTCAACGTCCGGCGTGGTGCCTGCGCTGGATAAGCTGGGCGATATGATAGATGTCGCGCTGGCTATCTCCTTACATGCG
CCGAATGACACTATTCGCGACGAAATCGTCCCGATCAACAAAAAATACAATATCGAAACGTTCCTGGGCGCGGTTCGCCG
CTATCTGGAGAAATCCAACGCCAATCAGGGGCGCGTGACGATTGAATACGTGATGCTTGACCACGTCAACGACGGCACTG
AACACGCGCATCAACTGGCGGAGCTACTAAAAGAGACGCCTTGTAAGATTAATCTGATTCCGTGGAACCCGTTCCCTGGC
GCGCCGTATGGCCGCAGCTCTAACAGCCGTATCGATCGCTTTTCTAAAGTTTTGATGAGTTATGGTTTCACGACCATCGT
ACGCAAGACGCGTGGCGATGACATTGATGCGGCATGCGGACAGTTGGCAGGCGATGTGATTGACCGTACTAAACGTACCC
TGCGTAAGCGTATGCAGGGAGAGGTGATTGACATCAAGGCTATTTGA

Upstream 100 bases:

>100_bases
TACAATGCAGCGCCCCTGGACGAGCAGTAAGCTTACCGGGGCGTTTCTTTTTTCAACCCTCCAGGGGCCATAACGTGTAA
TAACGAGGCCGGAATAGCAT

Downstream 100 bases:

>100_bases
TAGCAGTTGCGCCACGGTGCATTCATTATACCGTGGCGCAATGTCAGTTGAATAATGTCTGGCGTTGTACTTGTCTGGTC
AATAATAACGGTGCGTTTTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAE
IRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAA
KIVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
PNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPG
APYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEVIDIKAI

Sequences:

>Translated_388_residues
MSEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAE
IRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAA
KIVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
PNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPG
APYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEVIDIKAI
>Mature_387_residues
SEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEI
RAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAK
IVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAP
NDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPGA
PYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEVIDIKAI

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=388, Percent_Identity=95.3608247422681, Blast_Score=768, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43513; Mature: 43381

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDN
CCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
FDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRAT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEE
LCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMM
EEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEEC
PNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLA
CCCCHHHCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACG
HHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHH
QLAGDVIDRTKRTLRKRMQGEVIDIKAI
HHHHHHHHHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure 
SEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDN
CCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
FDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRAT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEE
LCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMM
EEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEEC
PNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLA
CCCCHHHCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHH
ELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACG
HHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHH
QLAGDVIDRTKRTLRKRMQGEVIDIKAI
HHHHHHHHHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA