Click here to switch to the map view.

The map label for this gene is yiaJ [H]

Identifier: 194737487

GI number: 194737487

Start: 3732803

End: 3733603

Strand: Reverse

Name: yiaJ [H]

Synonym: SeSA_A3865

Alternate gene names: 194737487

Gene position: 3733603-3732803 (Counterclockwise)

Preceding gene: 194737463

Following gene: 194738110

Centisome position: 79.29

GC content: 52.56

Gene sequence:

>801_bases
ATGAGCCAGAACAACGATAAAGAGAAGCCTGCGGGGAGTCAGAGCCTGTTTCGTGGCCTGATGTTGATAGAAATTTTGAG
TAACTACCCGAACGGTTGCCCACTGGCGCACCTGTCGGAGCTGGCGGGGTTGAACAAGAGCACCGTCCATCGTTTGTTGC
AGGGGCTTCAATCCTGCGGATACGTGACGCCAGCGCCGGCGGCCGGGAGCTACCGTCTGACGACAAAGTTTATTGCCGTC
GGCCAAAAAGCCCTGTCATCGCTGAATATCATCCACGTTGCGGCACCGCACCTTGAGGCGCTCAACCTGACCACCGGCGA
GACGGTGAATTTTTCCAGCCGTGAAGATGATCACGCTATCCTGATTTATAAGCTGGAACCGACCACCGGTATGCTGCGCA
CGCGTGCCTACATTGGCCAGCATATGCCGCTGTACTGCTCGGCGATGGGGAAAATTTATATGGCGTTTGGTCAGCCGGAT
TATGTGGCGTCTTACTGGGAAAGCCATAAAGATCAGATCCAGCCGCTGACTCGCAACACCATTACCGATTTACCAGCAAT
GTACGATGAGCTGGCGCAAATTCGTGAAACCAGCATGGCAATGGATCGCGAAGAAAATGAACTGGGCGTTTCCTGTATTG
CTGTGCCTGTGTTTGATATTCACCATCGGGTGCCTTACGCCATTTCTATTTCACTGTCGACGTCACGGCTAAAACAGATC
GGCGATAAAAATCTGCTCAAACCGTTGCGTGAAACCGCACAGGCTATCTCTAATGAGCTGGGATTTACCGTTCGCGAATA
A

Upstream 100 bases:

>100_bases
ACGATTAAAATCATCGGGTTATGTTTTTTATGCTGATATCGTGAACTGCAACACACTTTGAGCTACCATCTGATCGCCCA
AGAAAGGAGAGAGTTTTCTG

Downstream 100 bases:

>100_bases
GACAGCTCACCAGGTTTTATCAGGCATCTGACAAAATTTGTCCGTTTGCGTAGTGGGATCAAAGCGGCGGGGAATGGCTC
TCTGGCATTCTGGCGTTTTA

Product: HTH-type transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSQNNDKEKPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTPAPAAGSYRLTTKFIAV
GQKALSSLNIIHVAAPHLEALNLTTGETVNFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGQPD
YVASYWESHKDQIQPLTRNTITDLPAMYDELAQIRETSMAMDREENELGVSCIAVPVFDIHHRVPYAISISLSTSRLKQI
GDKNLLKPLRETAQAISNELGFTVRE

Sequences:

>Translated_266_residues
MSQNNDKEKPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTPAPAAGSYRLTTKFIAV
GQKALSSLNIIHVAAPHLEALNLTTGETVNFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGQPD
YVASYWESHKDQIQPLTRNTITDLPAMYDELAQIRETSMAMDREENELGVSCIAVPVFDIHHRVPYAISISLSTSRLKQI
GDKNLLKPLRETAQAISNELGFTVRE
>Mature_265_residues
SQNNDKEKPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTPAPAAGSYRLTTKFIAVG
QKALSSLNIIHVAAPHLEALNLTTGETVNFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGQPDY
VASYWESHKDQIQPLTRNTITDLPAMYDELAQIRETSMAMDREENELGVSCIAVPVFDIHHRVPYAISISLSTSRLKQIG
DKNLLKPLRETAQAISNELGFTVRE

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=91.1877394636015, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.4661354581673, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788131, Length=262, Percent_Identity=30.1526717557252, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786715, Length=252, Percent_Identity=30.5555555555556, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.920704845815, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790752, Length=219, Percent_Identity=31.5068493150685, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788581, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693096, Length=154, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29451; Mature: 29320

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNNDKEKPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
YVTPAPAAGSYRLTTKFIAVGQKALSSLNIIHVAAPHLEALNLTTGETVNFSSREDDHAI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEECCCCCCCCCEE
LIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGQPDYVASYWESHKDQIQPLTRNT
EEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCHHHHHH
ITDLPAMYDELAQIRETSMAMDREENELGVSCIAVPVFDIHHRVPYAISISLSTSRLKQI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHCCCCEEEEEEECHHHHHHC
GDKNLLKPLRETAQAISNELGFTVRE
CCHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure 
SQNNDKEKPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
YVTPAPAAGSYRLTTKFIAVGQKALSSLNIIHVAAPHLEALNLTTGETVNFSSREDDHAI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEECCCCCCCCCEE
LIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGQPDYVASYWESHKDQIQPLTRNT
EEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCHHHHHH
ITDLPAMYDELAQIRETSMAMDREENELGVSCIAVPVFDIHHRVPYAISISLSTSRLKQI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHCCCCEEEEEEECHHHHHHC
GDKNLLKPLRETAQAISNELGFTVRE
CCHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]