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The map label for this gene is zraS [H]

Identifier: 194737287

GI number: 194737287

Start: 4254438

End: 4255835

Strand: Direct

Name: zraS [H]

Synonym: SeSA_A4384

Alternate gene names: 194737287

Gene position: 4254438-4255835 (Clockwise)

Preceding gene: 194734888

Following gene: 194736973

Centisome position: 90.35

GC content: 54.65

Gene sequence:

>1398_bases
GTGAGTTTTATACGATTACATAAAGATGCCGCTGCAACGTGGTTGAGTCGGCTGTTACCTGCGGCGATCTTCATTCTGGT
GGGGCTATTTTCCATTATGGTCATTCGCGACTATGGCCGTGAGAGCGCTGCCGCCAGGCAGACGCTGCTGGAAAAGGGAA
ATGTACTCATTCGCGCTCTGGAGTCCGGCACGCGAGTAGGTATGGGAATGCGGATGCATCACGCGCAGCAGCAGACATTG
CTGGAAGAGATGGCGGGTCAGCCAGGCGTGTTGTGGTTCGCCGTAACGGATGCGCAGGGGGTAATCATCACTCATAGCAA
CCCTGGCATGGTGGGTAAATCGCTCTATTCCGCATCTGAAATGCATCAACTAAACCCCGGGCCGCAGGAGCGTTGGCGGC
GTGTCGACGTTGCTGCTAACGGCGAGACGGTGCCTGCGCTGGAAATTTACCGCCAGTTCCAGCCGCTTTTCGGTATGAGA
GGTCACGGTATGAGGGGGCACGGCATGTCGCGTAGCGCCAATGACGACGAACCAGCGAAACAAACTATTTTTATTGCCTT
TGACGCCAGCGAGCTGGCCGCGACACAGGCAAGGGAGTGGCGTAATACGCTGATTGTTTTGTCTGCGTTAGCGACGGTGC
TGTTGGCAACGTTACTGGCGTTTTTTTGGCATCAGCGTTATCAGCGCTCGCACAGAGAACTGCTTGATGCAATGAAGCGT
AAAGAAAAGCTGGTGGCGATGGGGCATCTGGCGGCGGGCGTCGCGCACGAAATTCGTAACCCGCTCTCGTCCATCAAAGG
GCTGGCGAAATACTTTGCCGAGCGCACGCCAGCCGGCGGCGAGTCGCATGAACTGGCGCAGGTGATGGCCAAAGAGGCCG
ATCGTTTGAACCGGGTGGTAAGCGAATTGCTCGAACTGGTAAAGCCCGCGCATCTGACGCTCCAGGCGGTTAACCTCAAT
GATATTATTACTCACTCCCTGAATCTGGTCAGTCAGGATGCCCAAAGCAGAGAGATTCAGTTGAGATTCACGGCGAATGA
GACGCTTAAGCGTATTCAGGCTGACCCGGACAGGCTGACTCAGGTGCTGTTGAATTTGTACCTGAACGCTATTCATGCGA
TTGGCCGCCAGGGAACGATTACCGTAGAGGCAAAAGACAGTGGGACCGACCGCGTTATCATCACTGTTACCGATAGCGGG
AAAGGGATCGCCCCGGATCAGTTGGAGGCGATTTTTACACCTTATTTTACGACCAAAGCTGACGGGACAGGCCTGGGGCT
GGCGGTGGTGCAAAATATCATCGAACAGCATGGCGGCGCGATTAAGGTGAAAAGTATTGAGGGTAAAGGCGCGGTCTTTA
CTATCTGGTTGCCAGTGATAGCGAGACAACAGGATTAA

Upstream 100 bases:

>100_bases
CCGTATCCGAAAGGGTGTGCGACAACGCGCGGTTATGCGGAGCGCAGAATGGCATGATCTCTGCTTAAGTAAGGAAAATA
AAAACGAGCCGGGGAAGCCT

Downstream 100 bases:

>100_bases
CAAGGGTGATACGCGGAAAAATCGATATTCTGGTTGTGGATGATGATGTAAGCCACTGCACGATTTTACAGGCTCTGCTT
CGGGGTTGGGGCTATAACGT

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSFIRLHKDAAATWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTL
LEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSASEMHQLNPGPQERWRRVDVAANGETVPALEIYRQFQPLFGMR
GHGMRGHGMSRSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALATVLLATLLAFFWHQRYQRSHRELLDAMKR
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLN
DIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKDSGTDRVIITVTDSG
KGIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD

Sequences:

>Translated_465_residues
MSFIRLHKDAAATWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTL
LEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSASEMHQLNPGPQERWRRVDVAANGETVPALEIYRQFQPLFGMR
GHGMRGHGMSRSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALATVLLATLLAFFWHQRYQRSHRELLDAMKR
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLN
DIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKDSGTDRVIITVTDSG
KGIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
>Mature_464_residues
SFIRLHKDAAATWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTLL
EEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSASEMHQLNPGPQERWRRVDVAANGETVPALEIYRQFQPLFGMRG
HGMRGHGMSRSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALATVLLATLLAFFWHQRYQRSHRELLDAMKRK
EKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLND
IITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKDSGTDRVIITVTDSGK
GIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=468, Percent_Identity=70.9401709401709, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=39.6396396396396, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790300, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994928, Length=257, Percent_Identity=30.3501945525292, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=26.4462809917355, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=26.457399103139, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786600, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=26.5765765765766, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=27.6497695852535, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693157, Length=276, Percent_Identity=28.6231884057971, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=25.6916996047431, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082128, Length=220, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=223, Percent_Identity=26.457399103139, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790861, Length=212, Percent_Identity=26.4150943396226, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51245; Mature: 51114

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIRLHKDAAATWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRAL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
ESGTRVGMGMRMHHAQQQTLLEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSASE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHH
MHQLNPGPQERWRRVDVAANGETVPALEIYRQFQPLFGMRGHGMRGHGMSRSANDDEPAK
HHCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QTIFIAFDASELAATQAREWRNTLIVLSALATVLLATLLAFFWHQRYQRSHRELLDAMKR
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SELLELVKPAHLTLQAVNLNDIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLT
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHH
QVLLNLYLNAIHAIGRQGTITVEAKDSGTDRVIITVTDSGKGIAPDQLEAIFTPYFTTKA
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCC
DGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEHHHCCCC
>Mature Secondary Structure 
SFIRLHKDAAATWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRAL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
ESGTRVGMGMRMHHAQQQTLLEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSASE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHH
MHQLNPGPQERWRRVDVAANGETVPALEIYRQFQPLFGMRGHGMRGHGMSRSANDDEPAK
HHCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QTIFIAFDASELAATQAREWRNTLIVLSALATVLLATLLAFFWHQRYQRSHRELLDAMKR
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SELLELVKPAHLTLQAVNLNDIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLT
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHH
QVLLNLYLNAIHAIGRQGTITVEAKDSGTDRVIITVTDSGKGIAPDQLEAIFTPYFTTKA
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCC
DGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]