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The map label for this gene is menF [H]

Identifier: 194737064

GI number: 194737064

Start: 2423284

End: 2424579

Strand: Reverse

Name: menF [H]

Synonym: SeSA_A2538

Alternate gene names: 194737064

Gene position: 2424579-2423284 (Counterclockwise)

Preceding gene: 194736438

Following gene: 194737329

Centisome position: 51.49

GC content: 56.79

Gene sequence:

>1296_bases
GTGCATTCTATTACTACTGCGCTGGAAAATCTGACGCGTCAGCTATCGCAAGAGATACCGGCGACGCCAGGGCTTTGCGT
TTTCGATGCGCCTTTCCCTTTAAACGATGCCTTCGATGCATTGAGCTGGCTGGCCAGCCAGTCATCCTTTCCGCAATTCT
ACTGGCAACAGCGCAATGGCGACGAAGAAGCTGCCGTTCTCGGGGCAATCACCGTCTTTTCATCGCTCGATCTGGCGCAA
CGGTTTTTACGTCAGCACGCGCAGCCTGACCTGCGTATTTGGGGGCTGAATGCGTTCGAGCCGAAAGAGGGCTACTTATT
GCTGCCGCGGCTGGAGTGGCGACGTAGCGGTGGTACGGCGACCTTACGCTTACATCTGCACAGTGACGTTTCGCTACGCG
ATGATGCGCGCCAGGCGAAAGCGTTTCTCGCTTCTCTGGTCGGTATTAAGCCTGTTCCGGCGCTGCGCCTTTCGCTCACG
AGCGAACAGCACTGGCCGGACAAAGACGGTTGGGTGAAGCTGATTCAGCAGGCCACCCACACCATTGCGCAAGAGGCGTT
TGATAAAGTTGTGCTGGCGCGGGCCACCGACTTACAATTCTCCCGTCCGGTCAATGCCGCCGCGATGATGGCGTCCAGTC
GTCGTTTAAATCTCAACTGCTACCACTTTTTTATGGCCTTTTCCGCCGATACCGCCTTTCTGGGATCATCGCCGGAGCGG
CTCTGGCGGCGGCGTGAAACGGCGCTACGTACCGAAGCGCTGGCCGGGACGGTGGCGAACCATCCGGATAACCATAAAGC
CTGGCAGCTTGGCGAATGGTTAATGAAAGACGATAAAAACCAGCGAGAAAATATGCTGGTTGTCGAAGACATCTGCCAGC
GACTGCAAAACTGCACCCACTCGCTGGATGTGCTGCCGCCGCAGGTGCTCAGACTGCGCAAGGTACAGCATTTGCGCCGC
TGTATCTGGACGGCGCTTAATCAGGCGGACGACACGCTCTGCCTGCACCAGTTACAGCCTACCGCAGCGGTCGCGGGGAT
CCCTCGTGAACCCGCGCGTTGTTTTATAGAACAACATGAGCCGTTCGAACGTGAATGGTATGCCGGGTCGGCAGGCTATC
TCTCCACGCGGCAAAGTGAGTTTTGCGTGACGTTACGTTCAGCCAAAGTGACGGCCAATGTGGTGCGATTGTATGCCGGC
GCGGGAATCGTCCGCGGCTCCGATCCGGAACAGGAATGGCAGGAAATAGATAATAAAGCGGCCGGGTTACGTACCTTATT
GCAGATGGATGTGTAA

Upstream 100 bases:

>100_bases
ATCCCCCCACTGAACAGAATTGCAACCCTCCCTAAATGAGGTACTGCTTTTTTAGGGCAGTACCCCGTATAATGTGGGGT
TTTTAACAGGGAGGGTCCGC

Downstream 100 bases:

>100_bases
TGAGTCACATATTCCCGACCCATATCAATAATCCATTTTTGCAGCTCTTTATACTTAGTCCCAATGATTGATACCGGACA
AACTCATGTCAGTAAGTGCA

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHSITTALENLTRQLSQEIPATPGLCVFDAPFPLNDAFDALSWLASQSSFPQFYWQQRNGDEEAAVLGAITVFSSLDLAQ
RFLRQHAQPDLRIWGLNAFEPKEGYLLLPRLEWRRSGGTATLRLHLHSDVSLRDDARQAKAFLASLVGIKPVPALRLSLT
SEQHWPDKDGWVKLIQQATHTIAQEAFDKVVLARATDLQFSRPVNAAAMMASSRRLNLNCYHFFMAFSADTAFLGSSPER
LWRRRETALRTEALAGTVANHPDNHKAWQLGEWLMKDDKNQRENMLVVEDICQRLQNCTHSLDVLPPQVLRLRKVQHLRR
CIWTALNQADDTLCLHQLQPTAAVAGIPREPARCFIEQHEPFEREWYAGSAGYLSTRQSEFCVTLRSAKVTANVVRLYAG
AGIVRGSDPEQEWQEIDNKAAGLRTLLQMDV

Sequences:

>Translated_431_residues
MHSITTALENLTRQLSQEIPATPGLCVFDAPFPLNDAFDALSWLASQSSFPQFYWQQRNGDEEAAVLGAITVFSSLDLAQ
RFLRQHAQPDLRIWGLNAFEPKEGYLLLPRLEWRRSGGTATLRLHLHSDVSLRDDARQAKAFLASLVGIKPVPALRLSLT
SEQHWPDKDGWVKLIQQATHTIAQEAFDKVVLARATDLQFSRPVNAAAMMASSRRLNLNCYHFFMAFSADTAFLGSSPER
LWRRRETALRTEALAGTVANHPDNHKAWQLGEWLMKDDKNQRENMLVVEDICQRLQNCTHSLDVLPPQVLRLRKVQHLRR
CIWTALNQADDTLCLHQLQPTAAVAGIPREPARCFIEQHEPFEREWYAGSAGYLSTRQSEFCVTLRSAKVTANVVRLYAG
AGIVRGSDPEQEWQEIDNKAAGLRTLLQMDV
>Mature_431_residues
MHSITTALENLTRQLSQEIPATPGLCVFDAPFPLNDAFDALSWLASQSSFPQFYWQQRNGDEEAAVLGAITVFSSLDLAQ
RFLRQHAQPDLRIWGLNAFEPKEGYLLLPRLEWRRSGGTATLRLHLHSDVSLRDDARQAKAFLASLVGIKPVPALRLSLT
SEQHWPDKDGWVKLIQQATHTIAQEAFDKVVLARATDLQFSRPVNAAAMMASSRRLNLNCYHFFMAFSADTAFLGSSPER
LWRRRETALRTEALAGTVANHPDNHKAWQLGEWLMKDDKNQRENMLVVEDICQRLQNCTHSLDVLPPQVLRLRKVQHLRR
CIWTALNQADDTLCLHQLQPTAAVAGIPREPARCFIEQHEPFEREWYAGSAGYLSTRQSEFCVTLRSAKVTANVVRLYAG
AGIVRGSDPEQEWQEIDNKAAGLRTLLQMDV

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=78.6542923433875, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=30.4347826086957, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788114, Length=209, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48758; Mature: 48758

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSITTALENLTRQLSQEIPATPGLCVFDAPFPLNDAFDALSWLASQSSFPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
DEEAAVLGAITVFSSLDLAQRFLRQHAQPDLRIWGLNAFEPKEGYLLLPRLEWRRSGGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCHHHHCCCCCE
TLRLHLHSDVSLRDDARQAKAFLASLVGIKPVPALRLSLTSEQHWPDKDGWVKLIQQATH
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHH
TIAQEAFDKVVLARATDLQFSRPVNAAAMMASSRRLNLNCYHFFMAFSADTAFLGSSPER
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCHHCCCCHHH
LWRRRETALRTEALAGTVANHPDNHKAWQLGEWLMKDDKNQRENMLVVEDICQRLQNCTH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCEEHHHHHHHHHHHHHH
SLDVLPPQVLRLRKVQHLRRCIWTALNQADDTLCLHQLQPTAAVAGIPREPARCFIEQHE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCHHHHHHHCCC
PFEREWYAGSAGYLSTRQSEFCVTLRSAKVTANVVRLYAGAGIVRGSDPEQEWQEIDNKA
CCCHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHH
AGLRTLLQMDV
HHHHHHHHHCC
>Mature Secondary Structure
MHSITTALENLTRQLSQEIPATPGLCVFDAPFPLNDAFDALSWLASQSSFPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
DEEAAVLGAITVFSSLDLAQRFLRQHAQPDLRIWGLNAFEPKEGYLLLPRLEWRRSGGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCHHHHCCCCCE
TLRLHLHSDVSLRDDARQAKAFLASLVGIKPVPALRLSLTSEQHWPDKDGWVKLIQQATH
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHH
TIAQEAFDKVVLARATDLQFSRPVNAAAMMASSRRLNLNCYHFFMAFSADTAFLGSSPER
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCHHCCCCHHH
LWRRRETALRTEALAGTVANHPDNHKAWQLGEWLMKDDKNQRENMLVVEDICQRLQNCTH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCEEHHHHHHHHHHHHHH
SLDVLPPQVLRLRKVQHLRRCIWTALNQADDTLCLHQLQPTAAVAGIPREPARCFIEQHE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCHHHHHHHCCC
PFEREWYAGSAGYLSTRQSEFCVTLRSAKVTANVVRLYAGAGIVRGSDPEQEWQEIDNKA
CCCHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHH
AGLRTLLQMDV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]