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The map label for this gene is ulaC [H]

Identifier: 194736612

GI number: 194736612

Start: 2459786

End: 2460229

Strand: Reverse

Name: ulaC [H]

Synonym: SeSA_A2573

Alternate gene names: 194736612

Gene position: 2460229-2459786 (Counterclockwise)

Preceding gene: 194736221

Following gene: 194734048

Centisome position: 52.24

GC content: 50.0

Gene sequence:

>444_bases
ATGCTGGGAACCTGGTTATCTGACGCAACCATCACGCTACAGGAGAGCGTGGAAACGTGGCCGCAGGCGCTGGAAATTTG
CGGCAAACCCCTGCTGGACGCAGGTGTTATCGCACCGGAATACATTACGGCGATTGTTCAGCAGCACCAAAAGCTTGGGC
CGTATTATGTATTAGCGCCAGGGTTAGCGATGCCGCATGCGAGGCCGGAAGAGGGGGCGAAAGGGTTAGGTTTATCGTTA
CTGAAACTACAGCGCGGCGTTTCATTTGGCGCAGACGAATTCGATCCGGTTGACATCATTATTATGCTGGCGGCGCCTGA
TAAGCACAGTCATATCGAAATGATATCGGCGCTGGCGGAATTATTTTCCAGTGATGTAGATATGGAAAAATTACATCAGG
CAAAAAACCTGGAGGACATTAAAACGATTATTGACCGTTTCTGA

Upstream 100 bases:

>100_bases
AGTCGCTGGTAGCGCTATCTTTGTGATAACGATCGCAGCTAATATCGAATGGCTTGAATAAAGTTTGTTCATAATTTCAT
ACACCGTGGGGGCGAATAAC

Downstream 100 bases:

>100_bases
TTTTATTCCATTTTCTTGCGGTAAATAACATTACGCCCGTGCGTGATGCAGACCAACTCTACTTTTAAAAGGTGACAATA
ATGAAAATCATGGCAATTTG

Product: PTS family enzyme IIA, mannitol-specific, cryptic

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLGTWLSDATITLQESVETWPQALEICGKPLLDAGVIAPEYITAIVQQHQKLGPYYVLAPGLAMPHARPEEGAKGLGLSL
LKLQRGVSFGADEFDPVDIIIMLAAPDKHSHIEMISALAELFSSDVDMEKLHQAKNLEDIKTIIDRF

Sequences:

>Translated_147_residues
MLGTWLSDATITLQESVETWPQALEICGKPLLDAGVIAPEYITAIVQQHQKLGPYYVLAPGLAMPHARPEEGAKGLGLSL
LKLQRGVSFGADEFDPVDIIIMLAAPDKHSHIEMISALAELFSSDVDMEKLHQAKNLEDIKTIIDRF
>Mature_147_residues
MLGTWLSDATITLQESVETWPQALEICGKPLLDAGVIAPEYITAIVQQHQKLGPYYVLAPGLAMPHARPEEGAKGLGLSL
LKLQRGVSFGADEFDPVDIIIMLAAPDKHSHIEMISALAELFSSDVDMEKLHQAKNLEDIKTIIDRF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=140, Percent_Identity=40.7142857142857, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1790755, Length=114, Percent_Identity=37.719298245614, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1789302, Length=117, Percent_Identity=43.5897435897436, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16119; Mature: 16119

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTWLSDATITLQESVETWPQALEICGKPLLDAGVIAPEYITAIVQQHQKLGPYYVLAP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECC
GLAMPHARPEEGAKGLGLSLLKLQRGVSFGADEFDPVDIIIMLAAPDKHSHIEMISALAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LFSSDVDMEKLHQAKNLEDIKTIIDRF
HHHCCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MLGTWLSDATITLQESVETWPQALEICGKPLLDAGVIAPEYITAIVQQHQKLGPYYVLAP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECC
GLAMPHARPEEGAKGLGLSLLKLQRGVSFGADEFDPVDIIIMLAAPDKHSHIEMISALAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LFSSDVDMEKLHQAKNLEDIKTIIDRF
HHHCCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]