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The map label for this gene is folK [H]

Identifier: 194736442

GI number: 194736442

Start: 220030

End: 220509

Strand: Reverse

Name: folK [H]

Synonym: SeSA_A0211

Alternate gene names: 194736442

Gene position: 220509-220030 (Counterclockwise)

Preceding gene: 194737669

Following gene: 194738168

Centisome position: 4.68

GC content: 58.75

Gene sequence:

>480_bases
ATGACGATCGCGTATATCGCGCTTGGCAGTAATCTGGCCTCTCCTCTGGAGCAGGTCAATGCCGCCCTGAAGGCTATCGC
CAACATTCCCGATAGCCGTATCGTCGCGGTTTCTTCGTTTTACCGCACGCCGCCGCTTGGCCCACAGGATCAGCCCGATT
ACCTGAACGCCGCGGTGGCGCTGGATACCGCCCTCGCCCCCGAAGAATTGCTTAACCATACCCAGCGCATTGAGCTGCAA
CAGGGCCGGGTGCGCAAAGCGGAACGCTGGGGGCCGCGCACGCTGGACCTCGACATTATGCTGTTTGGCGATGAAGTCAT
TAACACCGACCGTCTGACGGTTCCGCACTACGACATGAAAAACCGCGGTTTTATGCTGTGGCCGCTGTTTGAGATCGCTC
CAAAACTGCGCTTCCCCGACGATGGCGTCGCGCTGACGCAGCTTGTTCATCAACATACCCCCGCACCAGCATACTGGTAA

Upstream 100 bases:

>100_bases
CGGAACAAAAAGGAATGCTCAACGAGCTGGACGACGATCCTGCTCCACGCCGCCGCCGTTCACGTCCGCGCAAACGCGCG
CCGCGCCGCGAGGGCACCGT

Downstream 100 bases:

>100_bases
TTTTTAATTAATATTATTTTTAAGAAACCTGTATTATTACTTCGCTCACTAATTAATGGAATAAATCCAATCGTTATTTT
CACTTAAAAGATTGTTTAGC

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTIAYIALGSNLASPLEQVNAALKAIANIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPKLRFPDDGVALTQLVHQHTPAPAYW

Sequences:

>Translated_159_residues
MTIAYIALGSNLASPLEQVNAALKAIANIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPKLRFPDDGVALTQLVHQHTPAPAYW
>Mature_158_residues
TIAYIALGSNLASPLEQVNAALKAIANIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPKLRFPDDGVALTQLVHQHTPAPAYW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=150, Percent_Identity=86, Blast_Score=268, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324073, Length=134, Percent_Identity=32.8358208955224, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17828; Mature: 17696

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAYIALGSNLASPLEQVNAALKAIANIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVA
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHH
LDTALAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCCCCCCCEECCCCCCC
NRGFMLWPLFEIAPKLRFPDDGVALTQLVHQHTPAPAYW
CCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TIAYIALGSNLASPLEQVNAALKAIANIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVA
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHH
LDTALAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCCCCCCCEECCCCCCC
NRGFMLWPLFEIAPKLRFPDDGVALTQLVHQHTPAPAYW
CCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]