Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 194737669

GI number: 194737669

Start: 220506

End: 221819

Strand: Reverse

Name: pcnB [H]

Synonym: SeSA_A0212

Alternate gene names: 194737669

Gene position: 221819-220506 (Counterclockwise)

Preceding gene: 194733989

Following gene: 194736442

Centisome position: 4.71

GC content: 56.24

Gene sequence:

>1314_bases
ATGACGATTATCCCGCGTGAACAGCACGCTATCTCCCGCAAAGATATCAGTGAAAATGCCCTCAAGGTACTGTACAGGCT
GAACAAAGCGGGCTATGAAGCCTACCTGGTCGGCGGCGGCGTCCGCGATCTTCTGCTCGGCAAAAAGCCGAAGGATTTCG
ACGTGACCACCAACGCAACACCGGATCAGGTACGGAAATTATTCCGCAATTGCCGTCTGGTGGGGCGTCGTTTCCGCCTG
GCTCACGTGATGTTTGGCCCGGAAATTATCGAAGTGGCAACGTTTCGTGGTCATCATGAAGGCAGTGAAAGCGACCGTAC
GACCTCCCAGCGTGGGCAAAACGGTATGCTGCTGCGCGATAATATCTTCGGCTCTATCGAAGAAGATGCCCAGCGCCGCG
ATTTCACCATCAACAGCCTTTACTACAGCGTGGCGGATTTTACCGTGCGCGATTACGTCGGCGGGATGCAGGATCTGCAA
GAAGGCGTGATTCGCCTGATCGGCAATCCGGAAACACGCTACCGCGAAGATCCGGTTCGAATGCTGCGCGCCGTGCGTTT
CGCTGCGAAGCTCAATATGCGTATCAGCCCTGAAACGGCTGAGCCAATCCCGCGTCTGGCAACCTTGCTAAACGACATTC
CTCCCGCGCGCCTGTTCGAAGAGTCGCTGAAGCTGTTGCAGGCGGGGAACGGTTTTGAAACCTATCAACAACTGCGGGAA
TACCACCTCTTCCAGCCGTTGTTTCCTACCATTACGCGTTATTTCACCGAAAACGGCGACAGCGCAATGGAACGCATCAT
TGCGCTGGTGTTGAAGAATACGGATAACCGCATCCGTAACGAAATGCGCGTTAATCCGGCATTTTTGTTTGCCGCCATGT
TCTGGTATCCGCTGCTGGAAATGGCGCAAAAAATCGCTCAGGAGAGCGGTCTGGCCTATTACGACGCTTTCGCGCTAGCC
ATGAATGACGTGCTGGATGAAGCCTGCCGTTCGCTGGCGATCCCGAAACGTCTGACCACGCTTACCCGTGATATTTGGCA
GCTTCAGTTACGGATGTCCCGTCGTCAGGGCAAACGCGCCTGGAAGCTGATGGAACATCCCAAATTCCGCGCCGCGTTTG
ATTTGCTGGAGCTGCGCGCTCAGGTGGAAAATAATACTGAGCTGCAACGTCTGGCGCAGTGGTGGGCCGAGTTTCAGGCT
TCCGCGCCGCCGGAACAAAAAGGAATGCTCAACGAGCTGGACGACGATCCTGCTCCACGCCGCCGCCGTTCACGTCCGCG
CAAACGCGCGCCGCGCCGCGAGGGCACCGTATGA

Upstream 100 bases:

>100_bases
ACTACCGAGGTGCGCTATTTTTACCCGAGTCGCTAATTTTTGCCGTAAGGTGTTAAGCCGCGAGGAGAGCGAGGCAGAAC
AAGCCGTCGCCCGTCCACAT

Downstream 100 bases:

>100_bases
CGATCGCGTATATCGCGCTTGGCAGTAATCTGGCCTCTCCTCTGGAGCAGGTCAATGCCGCCCTGAAGGCTATCGCCAAC
ATTCCCGATAGCCGTATCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRL
AHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQ
EGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLRE
YHLFQPLFPTITRYFTENGDSAMERIIALVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALA
MNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQA
SAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Sequences:

>Translated_437_residues
MTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRL
AHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQ
EGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLRE
YHLFQPLFPTITRYFTENGDSAMERIIALVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALA
MNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQA
SAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
>Mature_436_residues
TIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLA
HVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQE
GVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLREY
HLFQPLFPTITRYFTENGDSAMERIIALVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAM
NDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQAS
APPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=210, Percent_Identity=33.8095238095238, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=416, Percent_Identity=90.8653846153846, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=229, Percent_Identity=33.6244541484716, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=175, Percent_Identity=30.2857142857143, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=287, Percent_Identity=26.1324041811847, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281360112, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357337, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50666; Mature: 50535

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCC
PDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEC
NIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVR
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHH
MLRAVRFAAKLNMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLRE
HHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
YHLFQPLFPTITRYFTENGDSAMERIIALVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
MAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WKLMEHPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPR
HHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCH
RRRSRPRKRAPRREGTV
HHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
TIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCC
PDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEC
NIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVR
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHH
MLRAVRFAAKLNMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLRE
HHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
YHLFQPLFPTITRYFTENGDSAMERIIALVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
MAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WKLMEHPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPR
HHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCH
RRRSRPRKRAPRREGTV
HHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]