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The map label for this gene is mpl [H]

Identifier: 194736211

GI number: 194736211

Start: 4555617

End: 4556990

Strand: Direct

Name: mpl [H]

Synonym: SeSA_A4686

Alternate gene names: 194736211

Gene position: 4555617-4556990 (Clockwise)

Preceding gene: 194737100

Following gene: 194737508

Centisome position: 96.74

GC content: 58.3

Gene sequence:

>1374_bases
ATGCGCATTCATATTTTGGGAATTTGTGGCACTTTCATGGGGGGGCTGGCGATGCTGGCTCGCTCGCTTGGTCATGAAGT
AACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGTACCTTACTTGAGAAGCAAGGCATTGATCTGATCCAGGGTTATG
ACGCCAGCCAGCTCGATCCGCAGCCGGATCTGGTGATTATCGGCAATGCGATGACGCGCGGGAATCCGTGCGTGGAAGCG
GTGCTGGAAAAAAACATTCCCTTTATGTCTGGTCCACAGTGGCTGCACGACTTTGTGTTGCGCGATCGCTGGGTACTGGC
GGTCGCCGGTACCCACGGCAAAACCACGACCGCAGGCATGGCGACCTGGATACTGGAAGCGTGCGGGTACAAACCGGGCT
TTGTGATTGGCGGCGTACCGGGTAACTTTGAGGTTTCCGCGCGCCTGGGCGAGAGCCCGTTCTTTGTTATCGAAGCGGAT
GAGTACGACTGCGCGTTCTTTGATAAACGTTCTAAATTTGTGCATTACTGCCCGCGGACGCTGATCCTCAACAACCTTGA
GTTTGATCATGCCGACATCTTCGACGATCTGAAAGCGATCCAGAAGCAGTTCCACCATCTGGTGCGCATCGTACCGGGGC
AGGGGCGCATCATCTGGCCGGAAAACGACATCAACCTGAAACAGACCATGGCGCTGGGCTGCTGGAGCGAGCAGGAGCTG
GTGGGCGAGCAGGGACACTGGCAGGCGAAGAAGCTGACTACGGATGCTTCCGAGTGGGAAGTGTGGCTGGACGGCGAAAA
AGTCGGCGATGTGAAATGGGGCCTGGTCGGCGAACATAACATGCACAACGGTCTGATGGCGATTGCCGCCGCGCGCCACG
TGGGCGTCGCGCCCGCAGAAGCGGCCAGTGCTCTGGGGTCATTTATTAACGCCCGCCGCCGTCTGGAACTGCGCGGTGAA
GCGAACGGCGTGACGGTGTATGACGATTTTGCTCATCACCCGACGGCAATTCTGGCCACGCTGGCGGCGCTGCGCGGTAA
AGTGGGCGGCACCGCGCGCATTATCGCAGTGCTGGAGCCGCGTTCTAACACGATGAAGATGGGGTTATGCAAGGATGACC
TGGCACCGTCGTTAGGGCGTGCGGATGAAGTGTTCCTGCTGCAGCCGCCGCATATTCCGTGGCAAGTGGCGGAAGTCGCT
GAAGCCTGCGTACAGCCTGCTCACTGGAGTGGCGATGTCGATACGCTGGCGGAAATGGTGGTGAAAACCGCACAGCCTGG
CGATCACATCCTGGTGATGAGCAACGGCGGTTTCGGCGGCATCCATCAGAAATTGCTGGATGGGCTGGCGAAAAAAGCAC
TGGTTGTGGAGTAA

Upstream 100 bases:

>100_bases
GTGAATCGCGCCAGCAAAGTACAGATTTACCTGAAATGCGTTTCTCACTTGCCAGACATGTGCGTAAAATGGCTCGCAGA
TAAAAAAAGGATAGTGACGT

Downstream 100 bases:

>100_bases
AAAATGCCCGGTGGCGCTGCGCTTACCGGGCCTACAATACGTACATTCCTGTAGGCCGGATAAGGCGCCAGCGCCGCCAT
CCGGCAGACGCTACCAACGT

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLEKQGIDLIQGYDASQLDPQPDLVIIGNAMTRGNPCVEA
VLEKNIPFMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEACGYKPGFVIGGVPGNFEVSARLGESPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMALGCWSEQEL
VGEQGHWQAKKLTTDASEWEVWLDGEKVGDVKWGLVGEHNMHNGLMAIAAARHVGVAPAEAASALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGLCKDDLAPSLGRADEVFLLQPPHIPWQVAEVA
EACVQPAHWSGDVDTLAEMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKALVVE

Sequences:

>Translated_457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLEKQGIDLIQGYDASQLDPQPDLVIIGNAMTRGNPCVEA
VLEKNIPFMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEACGYKPGFVIGGVPGNFEVSARLGESPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMALGCWSEQEL
VGEQGHWQAKKLTTDASEWEVWLDGEKVGDVKWGLVGEHNMHNGLMAIAAARHVGVAPAEAASALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGLCKDDLAPSLGRADEVFLLQPPHIPWQVAEVA
EACVQPAHWSGDVDTLAEMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKALVVE
>Mature_457_residues
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLEKQGIDLIQGYDASQLDPQPDLVIIGNAMTRGNPCVEA
VLEKNIPFMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEACGYKPGFVIGGVPGNFEVSARLGESPFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMALGCWSEQEL
VGEQGHWQAKKLTTDASEWEVWLDGEKVGDVKWGLVGEHNMHNGLMAIAAARHVGVAPAEAASALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGLCKDDLAPSLGRADEVFLLQPPHIPWQVAEVA
EACVQPAHWSGDVDTLAEMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKALVVE

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=453, Percent_Identity=96.4679911699779, Blast_Score=906, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=407, Percent_Identity=28.5012285012285, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49813; Mature: 49813

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLEKQGIDLIQGYDASQLDP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCC
QPDLVIIGNAMTRGNPCVEAVLEKNIPFMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEACGYKPGFVIGGVPGNFEVSARLGESPFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMALGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHEECCCCHHHH
VGEQGHWQAKKLTTDASEWEVWLDGEKVGDVKWGLVGEHNMHNGLMAIAAARHVGVAPAE
CCCCCCCCHHHCCCCCCCCEEEECCCEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCHH
AASALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGLCKDDLAPSLGRADEVFLLQPPHIPWQVAEVAEACVQPAHWSGDVDTLAEMV
CCCCEEECCCHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKALVVE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEECC
>Mature Secondary Structure
MRIHILGICGTFMGGLAMLARSLGHEVTGSDANVYPPMSTLLEKQGIDLIQGYDASQLDP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCC
QPDLVIIGNAMTRGNPCVEAVLEKNIPFMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEACGYKPGFVIGGVPGNFEVSARLGESPFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMALGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHEECCCCHHHH
VGEQGHWQAKKLTTDASEWEVWLDGEKVGDVKWGLVGEHNMHNGLMAIAAARHVGVAPAE
CCCCCCCCHHHCCCCCCCCEEEECCCEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCHH
AASALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGLCKDDLAPSLGRADEVFLLQPPHIPWQVAEVAEACVQPAHWSGDVDTLAEMV
CCCCEEECCCHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKALVVE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]