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The map label for this gene is pmbA [H]

Identifier: 194737508

GI number: 194737508

Start: 4557756

End: 4559096

Strand: Direct

Name: pmbA [H]

Synonym: SeSA_A4688

Alternate gene names: 194737508

Gene position: 4557756-4559096 (Clockwise)

Preceding gene: 194736211

Following gene: 194735427

Centisome position: 96.79

GC content: 56.23

Gene sequence:

>1341_bases
ATGAAAGTAATCTCACAAGTTGAAGCGCAGCGTAAGATTCTGGAAGAAGCAGTTTCGACAGCGCTGGAGTTAGCCTCAGG
CAAATCGGATGGGGCGGAAGTCGCCGTCAGCAAGACCACCGGCATTAGCGTCAGCACCCGTTATGGTGAAGTAGAGAATG
TCGAATTTAATAGCGACGGCGCGCTGGGCATTACGGTGTATCACCAGAACCGCAAAGGCAGCGCCTCTTCGACGGATTTA
AGCCCGCAGGCGATTGCCCGTACCGTGCAGGCCGCGCTGGATATCGCGCGTTACACGTCGCCGGACCCGTGCGCAGGCGT
GGCGGACAAAGAACTGCTGGCGTTTGACGCGCCGGATCTGGACTTGTTCCACCCGGCAGAGGTTACGCCAGACGAAGCCA
TTGAACTGGCCGCGCGTGCGGAACAGGCTTCTCTAAAGGCGGATAAACGCATCACCAATACCGAAGGCGGGAGCTTTAAC
AGCCATTACGGCATTAAAGTGTTCGGCAACAGCCACGGCATGTTGCAGGGATATTGCTCCACGCGCCATTCGCTCTCAAG
CTGCGTCATCGCTGAAGAGAACGGCGATATGGAGCGTGATTACGCTTACACCATCGGTCGGGCAATGGCGGATCTGCAAG
CGCCGGAATGGGTCGGGGCCGAGTGCGCGCGCCGTACCTTATCCCGCCTGTCGCCGCGCAAGCTCTCCACCATGAAAGCG
CCGGTCATTTTCGCCAATGAAGTGGCGACAGGGCTATTTGGTCATCTGGTCGGGGCGATTGCCGGCGGCGCGGTATACCG
TAAATCCACTTTTCTGCTCGACTCGCTGGGCAAACAAATTCTGCCGGAATGGTTAACCATCGAAGAACACCCGCATCTGC
TAAAAGGGCTGGCGTCTTCACCGTTCGACAGCGAAGGCGTGCGTACAGAGCGCCGGGACATTGTGAAGGATGGCGTACTG
ACGCAATGGTTGCTGACCAACTATTCCGCCCGCAAACTGGGGCTGAAAAGTACCGGTCATGCTGGCGGCATTCATAACTG
GCGTATCGCCGGGCAGGGGCTGAACTTTGAGCAGTTACTGAAAGAGATGGGCACCGGCCTGGTGGTGACGGAACTGATGG
GCCAGGGCGTCAGCGGTATTACCGGCGACTACTCCCGCGGCGCTGCGGGATTCTGGGTTGAAAATGGTGAAATCCAGTAC
CCGGTAAGTGAAATTACCATTGCCGGTAATTTAAAAGAGATGTGGCGTAATATTGTCACCGTGGGTGATGATATTGAAAC
GCGCAGTAATATACAGTGCGGTTCGGTATTGTTACCGGAGATGAAAATCGCCGGACAATAA

Upstream 100 bases:

>100_bases
TCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTC
TCTACATTATGGCATTTGCA

Downstream 100 bases:

>100_bases
TACCCGTCATACTTCAGGCTGCAGGCGCGTTGGCTGCCTGCAACTCGAATTATTCAGGGTATTACACTTATCGTTAATAA
TAAAAAAGGAAGTGAGCAAT

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFN
SHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLSTMKA
PVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVKDGVL
TQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ

Sequences:

>Translated_446_residues
MKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFN
SHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLSTMKA
PVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVKDGVL
TQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ
>Mature_446_residues
MKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFN
SHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLSTMKA
PVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVKDGVL
TQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=95.9641255605381, Blast_Score=897, Evalue=0.0,
Organism=Escherichia coli, GI1789640, Length=408, Percent_Identity=20.0980392156863, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47989; Mature: 47989

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEECCCC
ALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDL
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
DLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFNSHYGIKVFGNSHGMLQGYCS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHH
TRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLSTMKA
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
PVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASS
CEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHCCC
PFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHH
KEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKEMWRNIVT
HHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHHC
VGDDIETRSNIQCGSVLLPEMKIAGQ
CCCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure
MKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEECCCC
ALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDL
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
DLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFNSHYGIKVFGNSHGMLQGYCS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHH
TRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLSTMKA
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
PVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASS
CEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHCCC
PFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHH
KEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKEMWRNIVT
HHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHHC
VGDDIETRSNIQCGSVLLPEMKIAGQ
CCCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]