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The map label for this gene is waaA [H]

Identifier: 194736070

GI number: 194736070

Start: 3795424

End: 3796701

Strand: Direct

Name: waaA [H]

Synonym: SeSA_A3923

Alternate gene names: 194736070

Gene position: 3795424-3796701 (Clockwise)

Preceding gene: 194737936

Following gene: 194733976

Centisome position: 80.6

GC content: 55.01

Gene sequence:

>1278_bases
ATGCTCGAATTGCTTTACACCGCTCTTCTCTACCTTATTCAGCCTCTGATCTGGATACGGCTTTGGGTGCGTGGACGTAA
AGCGCCGGCCTATCGTAAACGCTGGGGTGAACGCTACGGATTCTACCGCCGTCCGTTGAAACCGGGCGGAATCATGCTGC
ATTCCGTCTCGGTGGGCGAAACTCTGGCGGCCATCCCATTGGTCCGCGCTCTACGTCATCGCTATCCCGATCTGCCTATT
ACCGTAACGACCATGACGCCGACCGGCTCGGAGCGCGTCCAGTCCGCCTTTGGCAACGATGTTCAGCACGTTTACTTGCC
TTATGATTTGCCCGATGCGCTCAATCGTTTCCTCAATGAGATTGATCCTAAGCTGGTATTGATCATGGAGACTGAGCTCT
GGCCAAATCTGATTGCTGCGCTGCACAAACGTCATATTCCGCTGGTTATCGCTAATGCGCGGCTTTCCGCCCGCTCCGCC
GCGGGTTATGCGAAGCTTGGCAAGTTTGTCCGTACGCTCCTGCGCCGTATCACCCTGATTGCCGCGCAAAACGAAGAAGA
TGGCGAACGCTTTGTGGCATTGGGCGCGAAGAACAATCAGGTCACGGTCACCGGCAGTCTGAAATTTGATATTTCAGTTA
CGCCGCAGCTGGCGGCTAAAGCCGTTACGCTACGCCGCCAGTGGGCGCCGCACCGTCCGGTCTGGATTGCCACCAGCACC
CACGATGGCGAAGAGAGTATCGTTATCGCCGCTCACCAGGCGTTATTACATCAATTCCCGAATTTATTACTGATTCTGGT
GCCCCGCCATCCAGAGCGTTTCCCGGATGCTATCAATCTTGTGCGTCAGGCAGGGTTAAGCTACATCACTCGCTCTTCGG
GCGAAGTGCCGTCCGCCAGCACCCAGGTCGTGGTAGGCGATACTATGGGCGAATTAATGTTGCTCTATGGCATTGCCGAT
CTCGCCTTTGTTGGTGGTTCGCTGGTTGAACGCGGCGGTCATAACCCGCTGGAGGCCGCCGCTCATGCGATTCCGGTACT
GATGGGTCCGCATACCTTTAACTTTAAAGATATTTGCGCCCGTCTGGATCAGGCGAGCGGACTTATCACGATTACCGATG
CGGCTACGCTGGCAAAAGAAGTTTCCTCTTTACTGACCGACGCTGATTATCGTAATTTCTACGGACGTCACGCAGTTGAA
GTGCTGTATCAAAATCAGGGCGCGCTCCAGCGTCTGCTGCAACTGCTGGAACCTTATCTGCCACCGAAAACGCATTGA

Upstream 100 bases:

>100_bases
TCGCTTCTGAGCCAATTTGGCGCAAAAATGGGAAAAGTAATGGTAAAGCCCAGGCTAAATACATAGAATCCCCAGCACAT
CCATAAGTCAGCTATTTACT

Downstream 100 bases:

>100_bases
GGACTGTCATGCAAAAACGGGCGATTTATCCAGGCACGTTTGATCCGATTACTAACGGTCATCTCGATATTGTGACGCGC
GCAACGCAGATGTTCGACCA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNEIDPKLVLIMETELWPNLIAALHKRHIPLVIANARLSARSA
AGYAKLGKFVRTLLRRITLIAAQNEEDGERFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HDGEESIVIAAHQALLHQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSASTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLDQASGLITITDAATLAKEVSSLLTDADYRNFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH

Sequences:

>Translated_425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNEIDPKLVLIMETELWPNLIAALHKRHIPLVIANARLSARSA
AGYAKLGKFVRTLLRRITLIAAQNEEDGERFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HDGEESIVIAAHQALLHQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSASTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLDQASGLITITDAATLAKEVSSLLTDADYRNFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH
>Mature_425_residues
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPI
TVTTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNEIDPKLVLIMETELWPNLIAALHKRHIPLVIANARLSARSA
AGYAKLGKFVRTLLRRITLIAAQNEEDGERFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HDGEESIVIAAHQALLHQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSASTQVVVGDTMGELMLLYGIAD
LAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLDQASGLITITDAATLAKEVSSLLTDADYRNFYGRHAVE
VLYQNQGALQRLLQLLEPYLPPKTH

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=425, Percent_Identity=96.4705882352941, Blast_Score=838, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47300; Mature: 47300

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
TLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNE
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
IDPKLVLIMETELWPNLIAALHKRHIPLVIANARLSARSAAGYAKLGKFVRTLLRRITLI
CCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHH
AAQNEEDGERFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
EECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECC
HDGEESIVIAAHQALLHQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSAS
CCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCC
TQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
CEEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHEEECCCCCCHHHHHH
RLDQASGLITITDAATLAKEVSSLLTDADYRNFYGRHAVEVLYQNQGALQRLLQLLEPYL
HHHHCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PPKTH
CCCCC
>Mature Secondary Structure
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRRPLKPGGIMLHSVSVGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
TLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGNDVQHVYLPYDLPDALNRFLNE
HHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
IDPKLVLIMETELWPNLIAALHKRHIPLVIANARLSARSAAGYAKLGKFVRTLLRRITLI
CCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHH
AAQNEEDGERFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
EECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECC
HDGEESIVIAAHQALLHQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSAS
CCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCC
TQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
CEEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHEEECCCCCCHHHHHH
RLDQASGLITITDAATLAKEVSSLLTDADYRNFYGRHAVEVLYQNQGALQRLLQLLEPYL
HHHHCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PPKTH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]