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The map label for this gene is cobI [H]

Identifier: 194736005

GI number: 194736005

Start: 2083233

End: 2083946

Strand: Reverse

Name: cobI [H]

Synonym: SeSA_A2193

Alternate gene names: 194736005

Gene position: 2083946-2083233 (Counterclockwise)

Preceding gene: 194737094

Following gene: 194735785

Centisome position: 44.25

GC content: 57.56

Gene sequence:

>714_bases
ATGAACGGTAAATTATACGCATTAAGTACCGGGCCAGGCGCGCCCGACCTGATAACCGTCCGCGCAGCGCGGATTCTTGG
CTCGCTGGACATTCTGTATGCGCCCGCCGGTCGTAAGGGGGGCGATAGTCTGGCACTGTCGATTGTGCGCGACTATCTCG
GCGAGCAGACGGAAGTACGTTGTTGCCATTTCCCTATGAGCGCTGACGGCGCCGAGAAAGAAGCTGTCTGGAACGAGGTT
GCCGCCGCGCTTACCGTAGAGGTGGAAGCGGGTAAACAGGTCGGTTTTATTACCCTGGGCGATGCGATGCTATTCAGTAC
CTGGATTTTTTTACTCCAGCGAGTCGGCTGCCCGGAGTGGCTGGAGATTGTCCCCGGCGTCACGTCCTTCGCCGCGATAG
CCGCCCGCGCGAAAATGCCGCTCGCCATAGAACAGCAATCGCTGGCGGTTATTTCTTGTACCGCGCCGGAAGCGGAAATA
GCCCAGGCGCTACAACAGCATGATAGCCTGGTGTTGATGAAAGTGTATGGCCGATTCGCCCGTATTAAAGCGTTGCTGGC
GCAGGCTGGCCTGCTGGAATGCGCCTTAATGATGTCCGAGGCCACGCTGCCCGGCGAGCAGTGCTGGCGCCATCTCCACG
AGGTCAACGATGATCGACCGTTGCCCTATTTTTCGACCATTCTGGTCAATAAACAGTGGGAGTATGCAGAATGA

Upstream 100 bases:

>100_bases
CGACGCCGTGGCTGAGCGGGCTGGGAGAAAACCCGGCTATCCGGGCGATGTTTGTCGCGCATTTGCACCAGGCGCTGAAC
CTGGCGGTAGAGGAGGCGGC

Downstream 100 bases:

>100_bases
AAATTGAACAGCAGCTTCGACAGTTGTCTTTCAGTGGACTGGCTGCAGCGCTATTGCTGATGGTTGTCCCGCAACAGGCG
TTTGCGATGCATATCATGGA

Product: cobalt-precorrin-2 C(20)-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTVEVEAGKQVGFITLGDAMLFSTWIFLLQRVGCPEWLEIVPGVTSFAAIAARAKMPLAIEQQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE

Sequences:

>Translated_237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTVEVEAGKQVGFITLGDAMLFSTWIFLLQRVGCPEWLEIVPGVTSFAAIAARAKMPLAIEQQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
>Mature_237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTVEVEAGKQVGFITLGDAMLFSTWIFLLQRVGCPEWLEIVPGVTSFAAIAARAKMPLAIEQQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 25792; Mature: 25792

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVR
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
CCHFPMSADGAEKEAVWNEVAAALTVEVEAGKQVGFITLGDAMLFSTWIFLLQRVGCPEW
EEECCCCCCCCHHHHHHHHHHHHEEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCHHH
LEIVPGVTSFAAIAARAKMPLAIEQQSLAVISCTAPEAEIAQALQQHDSLVLMKVYGRFA
HHHHCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHHHHHHHHHCCCEEEHHHHHHHH
RIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHEEEECCCCCCC
>Mature Secondary Structure
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVR
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
CCHFPMSADGAEKEAVWNEVAAALTVEVEAGKQVGFITLGDAMLFSTWIFLLQRVGCPEW
EEECCCCCCCCHHHHHHHHHHHHEEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCHHH
LEIVPGVTSFAAIAARAKMPLAIEQQSLAVISCTAPEAEIAQALQQHDSLVLMKVYGRFA
HHHHCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHHHHHHHHHCCCEEEHHHHHHHH
RIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]