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The map label for this gene is gph [H]

Identifier: 194735884

GI number: 194735884

Start: 3534251

End: 3535009

Strand: Reverse

Name: gph [H]

Synonym: SeSA_A3679

Alternate gene names: 194735884

Gene position: 3535009-3534251 (Counterclockwise)

Preceding gene: 194735372

Following gene: 194736932

Centisome position: 75.07

GC content: 55.99

Gene sequence:

>759_bases
ATGGATAAGTTGCAGAATATTCGGGGCGTCGCCTTTGATCTTGACGGCACGCTGGTGGATAGCGCGCCGGGTCTTGCCGC
GGCGGTGGATATGGCGCTGTATGCGCTGGAACTGCCGGTCGCGGGCGAGGAGCGCGTGATTACCTGGATTGGTAACGGCG
CAGACGTATTGATGGAACGCGCGCTGGCCTGGGCTCGCGAGGAGCGCGCCACGCTGCGTAAGACGATGGGGAAACCGCTC
GTTGATGAAGATATTCCTGCCGAGGAACAGGTACGCATTCTGCGTAAACTGTTCGACAGGTATTATGGCGAAGTGGCGGA
AGAAGGCACCTTTTTATTTCCGCATGTCGCCGACACGCTGGGCGCGCTGCACGCCAGCGGATTGTCATTAGGTCTGGTGA
CGAATAAGCCGACGCCGTTCGTCGCGCCGTTGCTGGAATCGCTTGATATCGCCAAATACTTTAGTGTGGTTATCGGTGGC
GATGATGTGCAAAATAAGAAGCCGCATCCGGAGCCGCTGTTGCTGGTGGCAAGCCGGCTGGGCATGATGCCGGAGCAGAT
GCTTTTTGTCGGCGATTCGCGTAATGATATTCAGGCTGCAAAAGCGGCGGGCTGCCCTTCGGTTGGCCTGACATACGGCT
ACAATTATGGCGAAGCGATCGCTCTTAGCGAGCCGGACGTCATTTATGACAGTTTTAACGATCTTTTGCCCGCACTTGGG
CTTCCGCATAGCGATAACCAGGAAATAAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCAGGCGCGGACATGTTTGTTGCGGGGTCGGCAATTTTCGATAAGCCCGACTACAAAAAAGTGATTGATGAAATGCGCAG
TGAGCTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGCAACTATATGGGGGCATTGCGTCAGTGGGTAA
ACATGCAGGATGACTATCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWAREERATLRKTMGKPL
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMMPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND

Sequences:

>Translated_252_residues
MDKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWAREERATLRKTMGKPL
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMMPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND
>Mature_252_residues
MDKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWAREERATLRKTMGKPL
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMMPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=90.0793650793651, Blast_Score=466, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27337; Mature: 27337

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALAWAREERATLRKTMGKPLVDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGGDDVQNKKPHPEPLLLVASRL
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHH
GMMPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIALSEPDVIYDSFNDLLPALG
CCCCHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHHHC
LPHSDNQEIKND
CCCCCCCCCCCC
>Mature Secondary Structure
MDKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALAWAREERATLRKTMGKPLVDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGGDDVQNKKPHPEPLLLVASRL
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHH
GMMPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIALSEPDVIYDSFNDLLPALG
CCCCHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHHHC
LPHSDNQEIKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]