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The map label for this gene is potI [H]

Identifier: 194735805

GI number: 194735805

Start: 1004807

End: 1005652

Strand: Direct

Name: potI [H]

Synonym: SeSA_A1033

Alternate gene names: 194735805

Gene position: 1004807-1005652 (Clockwise)

Preceding gene: 194737836

Following gene: 194734063

Centisome position: 21.34

GC content: 57.92

Gene sequence:

>846_bases
ATGAATAACTTACCGGTCGTTCGCTCGCCCTGGCGCATTCTGATTCTGGTGCTGGGGTTTACTTTTCTCTATGCGCCGAT
GTTAATGTTGGTGATTTACTCGTTTAACAGTTCGAAGCTGGTGACGGTGTGGGCGGGATGGTCGACGCGCTGGTATGGCG
AGCTCTTTCGGGATACCGCCATGATGAGCGCAGTGGGCCTAAGCCTGACAATTGCCGCCTGCGCGGCGACGATGGCGGTG
GTGCTCGGTACCATTGCCGCGGTGGTGATGGTGCGATTTGGACGGTTTCGCGGCGCAAACGGCTTTGCGTTTATGATCAC
CGCGCCGCTGGTGATGCCGGATGTGATTACCGGGCTGTCGCTGCTGCTGCTGTTTGTCGCGCTCGGTCATGCTATCGGCT
GGCCCTCCGATCGCGGGATGCTGACCATCTGGTTGGCGCACGTCACTTTCTGTACCGCCTATGTGGCAGTGGTGATCGCT
TCGCGATTACGCGAACTGGATCGTTCGATTGAAGAGGCGGCGATGGATCTTGGCGCGGCGCCGTTGAAGGTCTTTTTTGT
CATTACGTTACCGATGATCATGCCAGCGGTGATCTCGGGCTGGCTGTTGGCCTTTACGCTTTCCCTTGACGATCTGGTGA
TTGCCAGCTTCGTTTCCGGTCCCGGCGCCACCACGCTACCGATGCTAGTTTTCTCCAGCGTGCGCATGGGCGTGAACCCG
GAAATCAACGCGCTGGCGACGTTAATCCTCGGCGTGGTAGGAATCGTCGGATTTATTGCCTGGTACCTGATGGCGCGAGC
GGAAAAACAGCGGATTCGCGATATCCAGCGTGCAAGACGCGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGGCTTCTGCGGTCGCCATTATTATGTTGCTGCTGCTGATAGTACCGATCATGTGGTTCCATAAACATCAGCAA
AAAAGTGTGGGGGAGCACGG

Downstream 100 bases:

>100_bases
AGCCACTAAAATCTTCCACTATGGCAATGCGTTGCCGCGCGGGTCGCGGCATCATTTTCAGGGAAGACATTCATTGGGAT
TTATAAAGCAATCAACCTCA

Product: putrescine transporter subunit: membrane component of ABC superfamily protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPVVRSPWRILILVLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELFRDTAMMSAVGLSLTIAACAATMAV
VLGTIAAVVMVRFGRFRGANGFAFMITAPLVMPDVITGLSLLLLFVALGHAIGWPSDRGMLTIWLAHVTFCTAYVAVVIA
SRLRELDRSIEEAAMDLGAAPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGVVGIVGFIAWYLMARAEKQRIRDIQRARRG

Sequences:

>Translated_281_residues
MNNLPVVRSPWRILILVLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELFRDTAMMSAVGLSLTIAACAATMAV
VLGTIAAVVMVRFGRFRGANGFAFMITAPLVMPDVITGLSLLLLFVALGHAIGWPSDRGMLTIWLAHVTFCTAYVAVVIA
SRLRELDRSIEEAAMDLGAAPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGVVGIVGFIAWYLMARAEKQRIRDIQRARRG
>Mature_281_residues
MNNLPVVRSPWRILILVLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELFRDTAMMSAVGLSLTIAACAATMAV
VLGTIAAVVMVRFGRFRGANGFAFMITAPLVMPDVITGLSLLLLFVALGHAIGWPSDRGMLTIWLAHVTFCTAYVAVVIA
SRLRELDRSIEEAAMDLGAAPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGVVGIVGFIAWYLMARAEKQRIRDIQRARRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=94.661921708185, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=41.4225941422594, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=31.640625, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30607; Mature: 30607

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPVVRSPWRILILVLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELFRDTA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATMAVVLGTIAAVVMVRFGRFRGANGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
LLLLFVALGHAIGWPSDRGMLTIWLAHVTFCTAYVAVVIASRLRELDRSIEEAAMDLGAA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGVVGIVGFIAWYLMARAEKQRIRDIQRARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLPVVRSPWRILILVLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELFRDTA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATMAVVLGTIAAVVMVRFGRFRGANGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
LLLLFVALGHAIGWPSDRGMLTIWLAHVTFCTAYVAVVIASRLRELDRSIEEAAMDLGAA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGVVGIVGFIAWYLMARAEKQRIRDIQRARRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]