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The map label for this gene is selB [H]

Identifier: 194735632

GI number: 194735632

Start: 3750320

End: 3752170

Strand: Reverse

Name: selB [H]

Synonym: SeSA_A3882

Alternate gene names: 194735632

Gene position: 3752170-3750320 (Counterclockwise)

Preceding gene: 194733974

Following gene: 194735387

Centisome position: 79.68

GC content: 57.1

Gene sequence:

>1851_bases
ATGATTATTGCAACTGCCGGACACGTAGACCACGGTAAAACAACGTTGCTCCAGGCGATTACCGGGGTCAATGCCGACCG
TCTGCCGGAAGAGAAAAAACGCGGGATGACTATCGATCTGGGTTACGCCTATTGGCCGCAGCCGGATGGCCGTGTGCTGG
GCTTTATCGATGTGCCTGGGCATGAAAAATTCCTCTCTAACATGCTGGCGGGCGTAGGCGGTATCGATCACGCTTTGCTG
GTGGTCGCCTGTGATGACGGCGTCATGGCGCAAACGCGCGAGCATTTACAGATCCTGCAACTGACGGGCAATCTCCAGCT
CACCGTCGCGCTGACCAAAGCCGATCGCGTGGACGAGGCGCGTATCGGTGAAGTACGAGAAGAGGTACTGGCGGCGCTCG
ACAACTATGGCTTCGCCGATACCGTTTTATTTGTCACCGCTGCCAATGAAGGACACGGTATTGCAGAACTTCGCGCTCAT
TTACAGCAGCTTCCTGCACGTTTGCACGCCGCGCAGCATCGCTTCCGTCTGGCTATCGACCGTGCGTTTACGGTGAAAGG
GGCGGGACTGGTGGTGACCGGAACGGCGCTGAGCGGAGAGGTGAAGGTAGGTGATACGCTGTGGTTAACCGGAGTAAATA
AACCGATGCGGGTGCGTAGCCTGCACGCACAAAATCAGCCTACCGACCATGCGCACGCCGGGCAACGTATTGCGCTCAAT
ATTGCGGGCGATGCGGAGAAGGAACAGCTTAACCGCGGCGACTGGCTGCTTTCCGATGCGCCGGTGGGGGAAGCTTTTTC
CCGGGTGATCGTTTCTCTGGCACTTCATGCGCCGCTTTCCCAATGGCAGCCGCTACATATTCACCATGCCGCCAGCCATG
TGACCGGTCGCGTCTCTTTGCTGGAAGGCGGGCTGGCCGAGCTGATCTTCGATACGCCGTTGTGGCTGGCCGATAATGAC
AGACTGGTGCTGCGTGATATTTCCGCACGCGCCACCCTTGCCGGCGCACGCGTGGTGACGCTTAAGGCGCCGCGTCGCGG
TAAACGTAAGCCAGATTATTTGCACTGGCTTTCAACGCTTGCCGCCGCGCAGGACGATAGCGCGGCGTTGGCCATTCATC
TGGAACGCGGCGCCGTCAACCTGCCGGATTTCGGTTGGGCGCGTCAGCTTAATCCTCTCGGTATGCGTCAGCTTATTGAG
CAACATGGTTTTATCCAGGCCGGAGACAATCTGCTAAGCGCGCCCGTCGCCGCCCGCTGGCAGCGTAAGATTCTCGATAC
GCTGGCGACCTACCACGAACAGCACCGCGATGAACCGGGGCCGGGACGCGAGCGTTTACGCCGCATGGCCCTGCCAATGG
AAGATGAAGCGCTGGTCCTGATGCTGATCGAGCGGATGCGCGACGACGGCCTGATTCATAGCCATCACGGTTGGTTGCAT
CTGCCGGATCATAAAGCGGGATTTAGCGATGAGCAGCAGGCCGTCTGGCAAAAAGTTGAACCGCTATTTGGCGATGAGCC
CTGGTGGGTACGCGATCTGGCGAAAGAGACCGGAACGGAAGAACAATTGATGCGGCTGGTGCTGCGGCAGGCGGCACAGC
AAGGCATCATTACCGCGATCGTAAAAGATCGGTACTATCGTAACGATCGGATTGTCGCTTTCGCCAATATGATCCGCGAG
CTGGATCAAGAGAGAGGATCAACGTGTGCGGCGGATTTTCGCGACAGGCTGAATGTAGGGCGGAAACTGGCTATTCAGAT
TCTTGAATATTTTGACCGGATTGGATTTACACGACGCCGTGGGAATGAGCATTTATTACGTGATGCATTATTATTTCCGC
AAAAAGAATGA

Upstream 100 bases:

>100_bases
CGCTGCCTGTACCGGTCATTGGCCGAATTTATGATGGCCGTTTATGGCTGGATATGCGCTGTCTTGAAGACGAAAGCCGG
TTTATGGAGATGATGCTGAA

Downstream 100 bases:

>100_bases
AATGGTTAAATTAAATCAGAAATGAATCAATACAATTATTGAGTTGATTTATAAAAGCACATCAGGAAATTAACCTAATG
TGCTTTTTATTTTTCTGACT

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARIGEVREEVLAALDNYGFADTVLFVTAANEGHGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRSLHAQNQPTDHAHAGQRIALN
IAGDAEKEQLNRGDWLLSDAPVGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGGLAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLKAPRRGKRKPDYLHWLSTLAAAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDDGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNEHLLRDALLFPQKE

Sequences:

>Translated_616_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARIGEVREEVLAALDNYGFADTVLFVTAANEGHGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRSLHAQNQPTDHAHAGQRIALN
IAGDAEKEQLNRGDWLLSDAPVGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGGLAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLKAPRRGKRKPDYLHWLSTLAAAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDDGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNEHLLRDALLFPQKE
>Mature_616_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARIGEVREEVLAALDNYGFADTVLFVTAANEGHGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRSLHAQNQPTDHAHAGQRIALN
IAGDAEKEQLNRGDWLLSDAPVGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGGLAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLKAPRRGKRKPDYLHWLSTLAAAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDDGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNEHLLRDALLFPQKE

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=280, Percent_Identity=32.5, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI54607086, Length=283, Percent_Identity=25.7950530035336, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI5729864, Length=374, Percent_Identity=25.668449197861, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI223555963, Length=374, Percent_Identity=25.668449197861, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI53729339, Length=250, Percent_Identity=29.2, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI53729337, Length=250, Percent_Identity=29.2, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI46094014, Length=381, Percent_Identity=23.6220472440945, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI194097354, Length=298, Percent_Identity=24.1610738255034, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018520, Length=298, Percent_Identity=24.1610738255034, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018522, Length=302, Percent_Identity=23.841059602649, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367247, Length=615, Percent_Identity=85.0406504065041, Blast_Score=1065, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=369, Percent_Identity=28.7262872628726, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789737, Length=369, Percent_Identity=28.7262872628726, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789108, Length=331, Percent_Identity=27.190332326284, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789559, Length=96, Percent_Identity=39.5833333333333, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=276, Percent_Identity=32.2463768115942, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556456, Length=373, Percent_Identity=27.3458445040214, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17506081, Length=260, Percent_Identity=27.3076923076923, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=304, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115532065, Length=304, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17552884, Length=404, Percent_Identity=24.7524752475248, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17569207, Length=404, Percent_Identity=24.7524752475248, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=30.3418803418803, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=303, Percent_Identity=32.3432343234323, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325337, Length=408, Percent_Identity=23.7745098039216, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319594, Length=408, Percent_Identity=23.7745098039216, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320377, Length=281, Percent_Identity=24.1992882562278, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19921738, Length=264, Percent_Identity=32.1969696969697, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281363316, Length=279, Percent_Identity=31.5412186379928, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17864358, Length=279, Percent_Identity=31.5412186379928, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922690, Length=260, Percent_Identity=31.9230769230769, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137380, Length=351, Percent_Identity=24.2165242165242, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45553807, Length=317, Percent_Identity=26.1829652996845, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45553816, Length=317, Percent_Identity=26.1829652996845, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651721, Length=317, Percent_Identity=26.1829652996845, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864154, Length=317, Percent_Identity=26.1829652996845, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550900, Length=330, Percent_Identity=24.2424242424242, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652838, Length=406, Percent_Identity=23.6453201970443, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137572, Length=406, Percent_Identity=23.6453201970443, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28572034, Length=149, Percent_Identity=34.8993288590604, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 68688; Mature: 68688

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCEEEEEEEECCCCCCHH
RIGEVREEVLAALDNYGFADTVLFVTAANEGHGIAELRAHLQQLPARLHAAQHRFRLAID
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
RAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRSLHAQNQPTDHAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
IAGDAEKEQLNRGDWLLSDAPVGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSL
ECCCCCHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHCCCEEEEHHHHHHHCCHHH
LEGGLAELIFDTPLWLADNDRLVLRDISARATLAGARVVTLKAPRRGKRKPDYLHWLSTL
HHCCHHHHHHCCCCEEECCCEEEEEECCCHHEECCCEEEEEECCCCCCCCCHHHHHHHHH
AAAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIEQHGFIQAGDNLLSAPVAARW
HHCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCEECCCCHHCCHHHHHH
QRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDDGLIHSHHGWLH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCEEE
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAI
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VKDRYYRNDRIVAFANMIRELDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRR
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GNEHLLRDALLFPQKE
CCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCEEEEEEEECCCCCCHH
RIGEVREEVLAALDNYGFADTVLFVTAANEGHGIAELRAHLQQLPARLHAAQHRFRLAID
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
RAFTVKGAGLVVTGTALSGEVKVGDTLWLTGVNKPMRVRSLHAQNQPTDHAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEEEE
IAGDAEKEQLNRGDWLLSDAPVGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSL
ECCCCCHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHCCCEEEEHHHHHHHCCHHH
LEGGLAELIFDTPLWLADNDRLVLRDISARATLAGARVVTLKAPRRGKRKPDYLHWLSTL
HHCCHHHHHHCCCCEEECCCEEEEEECCCHHEECCCEEEEEECCCCCCCCCHHHHHHHHH
AAAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIEQHGFIQAGDNLLSAPVAARW
HHCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCEECCCCHHCCHHHHHH
QRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDDGLIHSHHGWLH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCEEE
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAI
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VKDRYYRNDRIVAFANMIRELDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRR
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GNEHLLRDALLFPQKE
CCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]