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The map label for this gene is wcaL [H]

Identifier: 194735345

GI number: 194735345

Start: 2202985

End: 2204205

Strand: Reverse

Name: wcaL [H]

Synonym: SeSA_A2333

Alternate gene names: 194735345

Gene position: 2204205-2202985 (Counterclockwise)

Preceding gene: 194734951

Following gene: 194734129

Centisome position: 46.81

GC content: 56.67

Gene sequence:

>1221_bases
ATGAAAGTCAGCTTTTTTCTGCTGAAATTTCCACTCTCATCGGAAACCTTTGTGCTGAATCAGATTACTGCGTTTGTTGA
TATGGGCCATGAGGTGGAGATTGTCGCGTTACAAAAAGGCGATACCCAACATACTCACGCCGCCTGGGAGAAGTATGGCC
TGGCGGCGAAAACCCGCTGGTTACAGGATGAGCCCCAGGGACGGCTGGCGAAACTGCGCTACCGGGCATGTAAAACGCTG
CCGGGGCTGCATCGTGCGGTGACCTGGAAAGCGCTCAATTTTATCCGCTATGGCGATGAATCACGCAATTTGATCCTTTC
CGCGATTTGCGCGCAGGTGAGCCACCCTTTTGTGGCGGATGTGTTTATCGCGCACTTTGGTCCGGCGGGCGTGACGGCGG
CCAAACTACGCGAACTGGGCGTGCTTCGCGGCAAAATCGCGACTATTTTCCACGGGATTGATATTTCCAGCCGCGAAGTG
CTCAGTCATTACACGCCGGAGTATCAGCAGTTGTTTCGTCGTGGCGATCTGATGCTGCCCATCAGCGATCTGTGGGCCGG
TCGCCTGAAAAGTATGGGCTGCCCGCCGGAAAAGATTGCCGTTTCGCGCATGGGCGTCGACATGACGCGTTTTTCCCATC
GTCCGGTGAAAGCGCCAGGGATGCCGCTGGAGATGATTTCCGTCGCGCGCCTGACCGAGAAAAAAGGACTGCATGTGGCG
ATTGAAGCCTGTCGGCAACTGAAAGCGCAGGGCGTGGCGTTTCGCTACCGCATTCTGGGGATTGGCCCGTGGGAACGTCG
GCTGCGCACGCTCATCGAGCAGTATCAGTTAGAGGATGTCATTGAGATGCCGGGGTTTAAACCGAGCCATGAAGTGAAGG
CGATGCTGGATGACGCCGATGTTTTTTTGCTGCCGTCGATTACCGGTACGGATGGCGATATGGAAGGTATTCCGGTAGCG
CTGATGGAGGCGATGGCGGTGGGGATTCCCGTGGTGTCTACCGTGCATAGCGGCATTCCGGAACTGGTGGAGGCCGGCAA
ATCCGGCTGGCTGGTGCCGGAAAACGATGCGCAGGCGCTGGCGGCCCGACTCGCTGAGTTCAGCCGGATTGACCACGACA
CGCTGGAGTCGGTGATCACGCGCGCCCGTGAAAAAGTGGCGCAAGATTTTAACCAGCAGGTGATTAATCGCCAGTTAGCC
AGCCTGCTACAAACGATATAA

Upstream 100 bases:

>100_bases
TGCCTGCGCTAAAGGCAAAAGTGGCTAACGCCGTTACCGAGGAGCGGGCGAAAGGGTTCACGATGATCAAATCCGTTCTC
GACAGAATTCGGGAGGAACA

Downstream 100 bases:

>100_bases
ACGAGGTGGTATGCCCGCGACTAAATTCTCCCGACGAACCCTCCTGACGGCAGGTTCCGCGCTTGCTGTTCTTCCTTTTC
TGCGCGCCTTGCCGCTACAG

Product: putative colanic acid biosynthesis glycosyltransferase WcaL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVSFFLLKFPLSSETFVLNQITAFVDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAVTWKALNFIRYGDESRNLILSAICAQVSHPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQVINRQLA
SLLQTI

Sequences:

>Translated_406_residues
MKVSFFLLKFPLSSETFVLNQITAFVDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAVTWKALNFIRYGDESRNLILSAICAQVSHPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQVINRQLA
SLLQTI
>Mature_406_residues
MKVSFFLLKFPLSSETFVLNQITAFVDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAVTWKALNFIRYGDESRNLILSAICAQVSHPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQVINRQLA
SLLQTI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=85.9605911330049, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45253; Mature: 45253

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFVDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAVTWKALNFIRYGDESRNLILSAICAQVSHPFVAD
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLESVITRAREKVAQDFNQQVINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFVDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAVTWKALNFIRYGDESRNLILSAICAQVSHPFVAD
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLESVITRAREKVAQDFNQQVINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]