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The map label for this gene is wcaM [H]

Identifier: 194734129

GI number: 194734129

Start: 2201571

End: 2202974

Strand: Reverse

Name: wcaM [H]

Synonym: SeSA_A2332

Alternate gene names: 194734129

Gene position: 2202974-2201571 (Counterclockwise)

Preceding gene: 194735345

Following gene: 194736956

Centisome position: 46.78

GC content: 51.0

Gene sequence:

>1404_bases
ATGCCCGCGACTAAATTCTCCCGACGAACCCTCCTGACGGCAGGTTCCGCGCTTGCTGTTCTTCCTTTTCTGCGCGCCTT
GCCGCTACAGGCGCGTGAACCTCGCCAGACCGTCGATATTAAGGATTATCCGGCGGATGACGGTATTGCCTCGTTCAAAC
AGGCCTTCGCCGACGGACAGACCGTGGTCGTACCGCCAGGATGGGTGTGTGAAAATATCAATGCGGCGATAACGATCCCG
GCGGGAAAAACGCTGCGGGTACAGGGCGCGGTGCGTGGGAATGGCCGGGGACGGTTTATTTTGCAGGACGGGTGTCAGGT
GGTGGGGGAGCAGGGCGGCAGTCTGCACAATGTGACGATGGATGTTCGCGGGTCGGACTGTGTGATTAAAGGCGTGACGA
TGAGCGGCTTTGGCCCCGTCGCGCAAATTTTCATTGGTGGTAAGGAACCGCAGGTGATGCGTAATCTCATTATCGATGAC
ATCACCGTTACCCACGCCAACTACGCCATTCTCCGCCAGGGATTTCATAACCAAATGGACGGCGCGAGGATTACGCATAG
CCGCTTTAGCGATTTACAGGGGGACGCCATTGAGTGGAATGTCGCGATTCACGACCGCGACATCCTGATTTCCGATCATG
TCATCGAACGCATTGATTGTACCAATGGCAAAATCAACTGGGGGATCGGCATCGGGCTGGCGGGTAGCACCTATGACAAC
AGTTATCCTGAAGATCAGGCAGTAAAAAACTTTGTGGTGGCCAATATTACCGGATCTGATTGCCGACAGCTGGTGCACGT
AGAAAATGGCAAACATTTCGTCATTCGCAATGTCAAAGCCAAAAACATCACGCCCGATTTCAGTAAAAATGCGGGTATTG
ATAACGCAACGATCGCAATTTATGGCTGTGATAATTTCGTCATTGATAATATTGATATGACGAATAGTGCCGGGATGCTC
ATCGGCTATGGCGTCGTTAAAGGAAAATACCTGTCAATTCCGCAAAACTTTAAATTAAACGCTATTCGGTTGGATAATCG
CCAGGTTGCTTATAAATTACGCGGCATTCAAATTTCATCCGGTAACGCCCCCTCATTTGTTGCCATCACCAATGTACGGA
TGACGCGTGCTACGCTGGAACTGCATAATCAACCGCAGCACCTCTTTCTGCGCAATATCAACGTGATGCAAACTTCAGCG
ATTGGCCCGGCGTTAAAAATGCATTTCGATTTGCGTAAAGATGTCCGTGGTCAATTTATGGCCCGCCAGGACACGCTGCT
TTCCCTCGCTAATGTTCATGCCATCAATGAAAACGGGCAGAGTTCCGTGGATATCGACAGGATTAATCACCAAACCGTGA
ATGTCGAAGCAGTGAATTTTTCGCTGCCGAAGCGGGGAGGGTAA

Upstream 100 bases:

>100_bases
GTGATCACGCGCGCCCGTGAAAAAGTGGCGCAAGATTTTAACCAGCAGGTGATTAATCGCCAGTTAGCCAGCCTGCTACA
AACGATATAAACGAGGTGGT

Downstream 100 bases:

>100_bases
GTACCGCTATTTTTACGAAAATTCCTGGGAAAAAGTTGTTCATACTTAATGTTATGGTGCCGACTAAGACGTAATGTAGA
GCGTGCCATCATTATCCCTG

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPATKFSRRTLLTAGSALAVLPFLRALPLQAREPRQTVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIP
AGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTMDVRGSDCVIKGVTMSGFGPVAQIFIGGKEPQVMRNLIIDD
ITVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERIDCTNGKINWGIGIGLAGSTYDN
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPDFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGML
IGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNAPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSA
IGPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG

Sequences:

>Translated_467_residues
MPATKFSRRTLLTAGSALAVLPFLRALPLQAREPRQTVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIP
AGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTMDVRGSDCVIKGVTMSGFGPVAQIFIGGKEPQVMRNLIIDD
ITVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERIDCTNGKINWGIGIGLAGSTYDN
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPDFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGML
IGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNAPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSA
IGPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
>Mature_466_residues
PATKFSRRTLLTAGSALAVLPFLRALPLQAREPRQTVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIPA
GKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTMDVRGSDCVIKGVTMSGFGPVAQIFIGGKEPQVMRNLIIDDI
TVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERIDCTNGKINWGIGIGLAGSTYDNS
YPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPDFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGMLI
GYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNAPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAI
GPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=466, Percent_Identity=77.4678111587983, Blast_Score=758, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51049; Mature: 50918

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATKFSRRTLLTAGSALAVLPFLRALPLQAREPRQTVDIKDYPADDGIASFKQAFADGQ
CCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCC
TVVVPPGWVCENINAAITIPAGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTM
EEEECCCCEECCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCCEEEEEE
DVRGSDCVIKGVTMSGFGPVAQIFIGGKEPQVMRNLIIDDITVTHANYAILRQGFHNQMD
EECCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCEEEECCCHHHHHHCCCCCCC
GARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERIDCTNGKINWGIGIGLAGSTYDN
CCEEEHHHHHCCCCCEEEEEEEEECCEEEECCHHHHEEECCCCEEEEEEEEEECCCCCCC
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPDFSKNAGIDNATIAI
CCCCHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEE
YGCDNFVIDNIDMTNSAGMLIGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISS
EECCCEEEECCCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEECCCEEEEEEEEEEEEC
GNAPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAIGPALKMHFDLRKDVRGQFM
CCCCCEEEEEEEEEEEEEEEECCCCCEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHH
ARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
HHHHHHHHHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PATKFSRRTLLTAGSALAVLPFLRALPLQAREPRQTVDIKDYPADDGIASFKQAFADGQ
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCC
TVVVPPGWVCENINAAITIPAGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTM
EEEECCCCEECCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCCEEEEEE
DVRGSDCVIKGVTMSGFGPVAQIFIGGKEPQVMRNLIIDDITVTHANYAILRQGFHNQMD
EECCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCEEEECCCHHHHHHCCCCCCC
GARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERIDCTNGKINWGIGIGLAGSTYDN
CCEEEHHHHHCCCCCEEEEEEEEECCEEEECCHHHHEEECCCCEEEEEEEEEECCCCCCC
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPDFSKNAGIDNATIAI
CCCCHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEE
YGCDNFVIDNIDMTNSAGMLIGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISS
EECCCEEEECCCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEECCCEEEEEEEEEEEEC
GNAPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAIGPALKMHFDLRKDVRGQFM
CCCCCEEEEEEEEEEEEEEEECCCCCEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHH
ARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
HHHHHHHHHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]