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The map label for this gene is rfaJ [H]

Identifier: 194734939

GI number: 194734939

Start: 3787841

End: 3788854

Strand: Reverse

Name: rfaJ [H]

Synonym: SeSA_A3916

Alternate gene names: 194734939

Gene position: 3788854-3787841 (Counterclockwise)

Preceding gene: 194737566

Following gene: 194735458

Centisome position: 80.46

GC content: 34.02

Gene sequence:

>1014_bases
ATGGATTCATTTCCTGAGATAGAAATAGCTGAATATAAAGTTTTTGATGAAAGTAATAATAATAATGATGATAACGTATT
AAACATTTCTTATGGTGTTGATGAAAACTATCTTGATGGTGTGGGGGTATCAATTGCTTCAGTTGTATTAAATAATAATA
TCCCGCTCGCTTTCCATATTATTTGTGATTCATACTCCTCGTGTTTTGTAAAATATATAGAGCGTTTAGCCGTACAGCAT
CACATAAAAATTTCTCTTTATCTTATTAAAGTAGAAAGCCTTGAGGTATTGCCTCAAACTAAAGTATGGTCGAGAGCAAT
GTATTTTCGTTTATTTGCTTTCGATTATCTCAGCAAGAAGGTAAACACATTACTTTATTTGGATGCCGATGTTGTATGCA
AAGGATCTTTGCAAGATCTTTTACAGCTTGATCTGACAGAGAAGATCGCTGCGGTCGTAAAAGATGTTGATTCCATCCAG
AATAAGGTAAATGAGAGATTAAGAGCATTTAATTTACAAGGTGGTTATTTTAACTCCGGCGTGGTTTTTGTTAACCTGAA
ATTATGGAAAGAGAATGCCTTAACTGAAAAGGCATTTTTACTTCTGGCAGGTAAAGAGGCTGACTCTTTTAAATATCCCG
ATCAGGATGTTTTGAATATTCTCCTACAGGATAAAGTCATTTTTCTTCCGCGACCGTATAATACCATTTATACTATCAAA
AGTGAGTTGAAAGATAAGTCACATAAAAAATATAGCAATATAATTAATGATAATACTATTTTAATTCATTATACGGGCGC
TACAAAACCATGGCATGCCTGGGCAAATTATCCTTCAGTTATCTATTATAAAAATGCACGACTGAATTCGCCCTGGAAAG
ATTTTCCCGCAAAAGATGCGCGTACCATAGTTGAATTTAAGAAGCGATATAAACATCTTCTCGTGCAAGGTCATTACTTT
AAAGGCCTTCTGGCTGGAAGCGCATATCTTTATCGTAAAATTTTCCACAAATAA

Upstream 100 bases:

>100_bases
CAAAGCATATGTTTAATCAGAAGCATTATACTTCGGGTATAAATTATTATATAGCCTACTTTAAACGTAAACTTCTTGAA
TAAAACCCATAGGTGATGTA

Downstream 100 bases:

>100_bases
TTTCCTTCCCTAAATCTCCATTATGATTATTGAAAAAAAGGTTAAAAACTATACCGTCTTTGTCAAAAAAGACGGTGAAA
AATACATTGAGATATTCAAA

Product: lipopolysaccharide 1,2-glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MDSFPEIEIAEYKVFDESNNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSSCFVKYIERLAVQH
HIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQ
NKVNERLRAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIK
SELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYF
KGLLAGSAYLYRKIFHK

Sequences:

>Translated_337_residues
MDSFPEIEIAEYKVFDESNNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSSCFVKYIERLAVQH
HIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQ
NKVNERLRAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIK
SELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYF
KGLLAGSAYLYRKIFHK
>Mature_337_residues
MDSFPEIEIAEYKVFDESNNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSSCFVKYIERLAVQH
HIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQ
NKVNERLRAFNLQGGYFNSGVVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIK
SELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYF
KGLLAGSAYLYRKIFHK

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790056, Length=339, Percent_Identity=58.4070796460177, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790057, Length=316, Percent_Identity=36.7088607594937, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 38937; Mature: 38937

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFPEIEIAEYKVFDESNNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHI
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEE
ICDSYSSCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKK
EECCHHHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHH
VNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLRAFNLQGGYFNSG
HCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEECCC
VVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIK
EEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEH
SELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDA
HHHCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCHHH
RTIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKIFHK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSFPEIEIAEYKVFDESNNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHI
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEE
ICDSYSSCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKK
EECCHHHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHH
VNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLRAFNLQGGYFNSG
HCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEECCC
VVFVNLKLWKENALTEKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIK
EEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEH
SELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDA
HHHCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCHHH
RTIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKIFHK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]