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The map label for this gene is birA [H]

Identifier: 194334980

GI number: 194334980

Start: 2363754

End: 2364737

Strand: Reverse

Name: birA [H]

Synonym: Paes_2191

Alternate gene names: 194334980

Gene position: 2364737-2363754 (Counterclockwise)

Preceding gene: 194334981

Following gene: 194334979

Centisome position: 94.1

GC content: 54.57

Gene sequence:

>984_bases
ATGAACGATTCTACAATACGCATTCTTCATTGTTTGCGTGATGCTGACGGTTTTGTTTCGGGCGAGACGATCTGCCGGGA
GTTTGGTATGACGCGAAGCGCCGTATGGAAACATATCTCTAAGCTGCGTGCTCTGGGCTATGGTATCGATGCATTGACAG
GACGAGGTTACAGGCTTTCGGCTATGCCCGGTCTTCCTGTTGGTCCGGAGGTCGAGCCGTTGCTTGACACGTCGCGGGTT
GGTCGGGAGCTGTTCTTTTATCCGATCGCGGTATCGACCAACGCTCTGGCAAAGAGTTTTGCCCGCAACGGTGCGGGAGA
GGGAGCGGTGGTGACAGCCGATGCACAGAGCGGCGGCCGGGGGCGCATGCAGCGGGTATGGAGTTCCCCTGCCGGAGTCA
ATCTCTATTTTTCCGTTATTCTCCGGCCTTCGGTTTCTCCGTTGAGACTTCCGCAGATTCCGCTTCTTGTTGCGGCGGCT
CTTCACCGGGCGCTCTCTTTTTCTGTTCCCGGGCTTCAGGTGCTGATCAAATGGCCGAACGATATGCTGGTCAATGGCAG
GAAGATATGCGGTATTCTGTGCGAGATGGAGTCTGAGGCGGATATGGCGCATTTCGTGATTGCCGGAATCGGTATCAATG
TGAATCTTCGTGAGTTGCCTCATGAGATCCGGGGTATCGCGACATCTCTTTTTCTTGAAACCGGGGAGGAGTATTCCCGG
TCAGCACTTCTTGCGTCGTTTTTCAATGCTTTCGAGCCGATCTATGATGCCTGGCTTGAGGAGGAAGATCTCGGGCAGGT
GCTCGAATGTCTTGATGAGTACTCCTATCTCAAGGGCAGACTGGTCGAGGTTGAGCAGTTTAACCGGCGTCTCAGGGGCC
GGGCGGCAGGCATCAGCAGGACAGGGGAGCTGCTTCTTGATCTCGATAACGGTGAGCGACGGGTGATTTCATCAGGCGAA
GCGCATCTTTTTATTGAACACTGA

Upstream 100 bases:

>100_bases
GGCGCGTGTTTCTGTTAAGCAACGCTGTGGTTTGCAAACTTGTCAACCCTGTCGTAAATTCCGGTTGACGGTTTGATGTT
TGGTCTTTTTCAGGGGTATC

Downstream 100 bases:

>100_bases
CAATCTGTTGAAGGATATGAATGGAGCTGTAATTGATCCTCTTATCCAGGAAGCCTATGGCGTTCTGGAAACGGGCCGCT
CGGTCTCCTACGATCTGGCC

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNDSTIRILHCLRDADGFVSGETICREFGMTRSAVWKHISKLRALGYGIDALTGRGYRLSAMPGLPVGPEVEPLLDTSRV
GRELFFYPIAVSTNALAKSFARNGAGEGAVVTADAQSGGRGRMQRVWSSPAGVNLYFSVILRPSVSPLRLPQIPLLVAAA
LHRALSFSVPGLQVLIKWPNDMLVNGRKICGILCEMESEADMAHFVIAGIGINVNLRELPHEIRGIATSLFLETGEEYSR
SALLASFFNAFEPIYDAWLEEEDLGQVLECLDEYSYLKGRLVEVEQFNRRLRGRAAGISRTGELLLDLDNGERRVISSGE
AHLFIEH

Sequences:

>Translated_327_residues
MNDSTIRILHCLRDADGFVSGETICREFGMTRSAVWKHISKLRALGYGIDALTGRGYRLSAMPGLPVGPEVEPLLDTSRV
GRELFFYPIAVSTNALAKSFARNGAGEGAVVTADAQSGGRGRMQRVWSSPAGVNLYFSVILRPSVSPLRLPQIPLLVAAA
LHRALSFSVPGLQVLIKWPNDMLVNGRKICGILCEMESEADMAHFVIAGIGINVNLRELPHEIRGIATSLFLETGEEYSR
SALLASFFNAFEPIYDAWLEEEDLGQVLECLDEYSYLKGRLVEVEQFNRRLRGRAAGISRTGELLLDLDNGERRVISSGE
AHLFIEH
>Mature_327_residues
MNDSTIRILHCLRDADGFVSGETICREFGMTRSAVWKHISKLRALGYGIDALTGRGYRLSAMPGLPVGPEVEPLLDTSRV
GRELFFYPIAVSTNALAKSFARNGAGEGAVVTADAQSGGRGRMQRVWSSPAGVNLYFSVILRPSVSPLRLPQIPLLVAAA
LHRALSFSVPGLQVLIKWPNDMLVNGRKICGILCEMESEADMAHFVIAGIGINVNLRELPHEIRGIATSLFLETGEEYSR
SALLASFFNAFEPIYDAWLEEEDLGQVLECLDEYSYLKGRLVEVEQFNRRLRGRAAGISRTGELLLDLDNGERRVISSGE
AHLFIEH

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=318, Percent_Identity=28.6163522012579, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36004; Mature: 36004

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSTIRILHCLRDADGFVSGETICREFGMTRSAVWKHISKLRALGYGIDALTGRGYRLS
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEE
AMPGLPVGPEVEPLLDTSRVGRELFFYPIAVSTNALAKSFARNGAGEGAVVTADAQSGGR
CCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHCCCCCCCEEEECCCCCCC
GRMQRVWSSPAGVNLYFSVILRPSVSPLRLPQIPLLVAAALHRALSFSVPGLQVLIKWPN
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCC
DMLVNGRKICGILCEMESEADMAHFVIAGIGINVNLRELPHEIRGIATSLFLETGEEYSR
CCEECCHHHHHEEEECCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
SALLASFFNAFEPIYDAWLEEEDLGQVLECLDEYSYLKGRLVEVEQFNRRLRGRAAGISR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCC
TGELLLDLDNGERRVISSGEAHLFIEH
CCCEEEEECCCCEEEECCCCCEEEEEC
>Mature Secondary Structure
MNDSTIRILHCLRDADGFVSGETICREFGMTRSAVWKHISKLRALGYGIDALTGRGYRLS
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEE
AMPGLPVGPEVEPLLDTSRVGRELFFYPIAVSTNALAKSFARNGAGEGAVVTADAQSGGR
CCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHCCCCCCCEEEECCCCCCC
GRMQRVWSSPAGVNLYFSVILRPSVSPLRLPQIPLLVAAALHRALSFSVPGLQVLIKWPN
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCC
DMLVNGRKICGILCEMESEADMAHFVIAGIGINVNLRELPHEIRGIATSLFLETGEEYSR
CCEECCHHHHHEEEECCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
SALLASFFNAFEPIYDAWLEEEDLGQVLECLDEYSYLKGRLVEVEQFNRRLRGRAAGISR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHCCCCC
TGELLLDLDNGERRVISSGEAHLFIEH
CCCEEEEECCCCEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]