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The map label for this gene is bioB

Identifier: 194334979

GI number: 194334979

Start: 2362724

End: 2363737

Strand: Reverse

Name: bioB

Synonym: Paes_2190

Alternate gene names: 194334979

Gene position: 2363737-2362724 (Counterclockwise)

Preceding gene: 194334980

Following gene: 194334978

Centisome position: 94.06

GC content: 53.06

Gene sequence:

>1014_bases
ATGAATGGAGCTGTAATTGATCCTCTTATCCAGGAAGCCTATGGCGTTCTGGAAACGGGCCGCTCGGTCTCCTACGATCT
GGCCACTCGTCTTGCGACCCTGCGGGGTGAGGCGGTGGTGGATCTGCTTTCGCTCGCAAATAAAGTGAAAAATCGCTTTG
CTGCTCCCGACGGTGCACTGCACAGCTGTTCGATCCTCAATGCCAAATCGGGGGCTTGTTCTGAAAACTGTCGCTTCTGT
GCCCAGTCGCTCCATAATCAGGCGGATGTTGAATCGTATTCCCTGCTTGACCGCTCGAAGGTCTTCGAGGCGGCAGGCAG
GGTCTATGCCAAGGGGGTCCGTCATTTCGGGATTGTAACGAGCGGGTATGGGTACCGGACGGTGAATAAGGAGTTTCAGC
GGATAATCGATCTCATAACAGGCCTGCGGGAAGAGTACCCTGGTTTGAAGGTGTGTGCTTCACTTGGTGTGCTTGGCTCT
GATAGCGCAGCATTGCTTGCCGATGCAGGTATTGCGCATTACAACATCAACCTCCAGGTTTCCCCTGACCGTTACAGTGA
CCTGATTGCCGACAGTCATTCGCAGAATGATCGTATCGAGACCATCAGATTGCTGCGAAAGCACAGGATTGCAGTGTGCT
GCGGCGCTATTCTCGGCGTTGGAGAGACGATGCAGGAGAGAATCGATTTGATTTTTGTGCTCCAGGCGCTTGATGTCGCA
GTGATCCCTCTCAACGTTCTGGTTCCTGTCGATGGAACGCCGCTTGAAGGTTCGCTTCCGCTTGCTGTGAGTGATATTGT
GAAAACCTTCGCTCTTTGTCGTCTTGCTCATCCGGACAAGGTGATAAAATTTGCCGCCGGCAGGGAGACGGTGATGAAGG
ATTTTCAGGGCCTTCTCATGCTTGCTGGAGCAAACGGTTTTCTGACAGGTGGTTATCTGACAACAAGGGGACGCGATGTT
GGCGACGATCAGGCATTCATGGAGCAGCTCAAACATTTTTCGGGGAGGTCCTGA

Upstream 100 bases:

>100_bases
ACAGGGGAGCTGCTTCTTGATCTCGATAACGGTGAGCGACGGGTGATTTCATCAGGCGAAGCGCATCTTTTTATTGAACA
CTGACAATCTGTTGAAGGAT

Downstream 100 bases:

>100_bases
TGCTACCGGTTTTTGAGGCTATGGAGAAAGAGCTTGATGCGCTGCGGCGCCAGGAGCGTTTCAGGCGGCTTCCGGATACC
GGGGTACGGGACGGGAGATT

Product: Biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNGAVIDPLIQEAYGVLETGRSVSYDLATRLATLRGEAVVDLLSLANKVKNRFAAPDGALHSCSILNAKSGACSENCRFC
AQSLHNQADVESYSLLDRSKVFEAAGRVYAKGVRHFGIVTSGYGYRTVNKEFQRIIDLITGLREEYPGLKVCASLGVLGS
DSAALLADAGIAHYNINLQVSPDRYSDLIADSHSQNDRIETIRLLRKHRIAVCCGAILGVGETMQERIDLIFVLQALDVA
VIPLNVLVPVDGTPLEGSLPLAVSDIVKTFALCRLAHPDKVIKFAAGRETVMKDFQGLLMLAGANGFLTGGYLTTRGRDV
GDDQAFMEQLKHFSGRS

Sequences:

>Translated_337_residues
MNGAVIDPLIQEAYGVLETGRSVSYDLATRLATLRGEAVVDLLSLANKVKNRFAAPDGALHSCSILNAKSGACSENCRFC
AQSLHNQADVESYSLLDRSKVFEAAGRVYAKGVRHFGIVTSGYGYRTVNKEFQRIIDLITGLREEYPGLKVCASLGVLGS
DSAALLADAGIAHYNINLQVSPDRYSDLIADSHSQNDRIETIRLLRKHRIAVCCGAILGVGETMQERIDLIFVLQALDVA
VIPLNVLVPVDGTPLEGSLPLAVSDIVKTFALCRLAHPDKVIKFAAGRETVMKDFQGLLMLAGANGFLTGGYLTTRGRDV
GDDQAFMEQLKHFSGRS
>Mature_337_residues
MNGAVIDPLIQEAYGVLETGRSVSYDLATRLATLRGEAVVDLLSLANKVKNRFAAPDGALHSCSILNAKSGACSENCRFC
AQSLHNQADVESYSLLDRSKVFEAAGRVYAKGVRHFGIVTSGYGYRTVNKEFQRIIDLITGLREEYPGLKVCASLGVLGS
DSAALLADAGIAHYNINLQVSPDRYSDLIADSHSQNDRIETIRLLRKHRIAVCCGAILGVGETMQERIDLIFVLQALDVA
VIPLNVLVPVDGTPLEGSLPLAVSDIVKTFALCRLAHPDKVIKFAAGRETVMKDFQGLLMLAGANGFLTGGYLTTRGRDV
GDDQAFMEQLKHFSGRS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=290, Percent_Identity=30.3448275862069, Blast_Score=150, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321725, Length=297, Percent_Identity=29.6296296296296, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PROA2 (B4S690)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016839.1
- ProteinModelPortal:   B4S690
- SMR:   B4S690
- GeneID:   6459765
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_2190
- HOGENOM:   HBG405569
- OMA:   AGINICC
- ProtClustDB:   CLSK637131
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36401; Mature: 36401

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAVIDPLIQEAYGVLETGRSVSYDLATRLATLRGEAVVDLLSLANKVKNRFAAPDGAL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HSCSILNAKSGACSENCRFCAQSLHNQADVESYSLLDRSKVFEAAGRVYAKGVRHFGIVT
CCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
SGYGYRTVNKEFQRIIDLITGLREEYPGLKVCASLGVLGSDSAALLADAGIAHYNINLQV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHCCEEEEEEEEEE
SPDRYSDLIADSHSQNDRIETIRLLRKHRIAVCCGAILGVGETMQERIDLIFVLQALDVA
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHE
VIPLNVLVPVDGTPLEGSLPLAVSDIVKTFALCRLAHPDKVIKFAAGRETVMKDFQGLLM
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHEE
LAGANGFLTGGYLTTRGRDVGDDQAFMEQLKHFSGRS
EECCCCEEECCEEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNGAVIDPLIQEAYGVLETGRSVSYDLATRLATLRGEAVVDLLSLANKVKNRFAAPDGAL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HSCSILNAKSGACSENCRFCAQSLHNQADVESYSLLDRSKVFEAAGRVYAKGVRHFGIVT
CCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
SGYGYRTVNKEFQRIIDLITGLREEYPGLKVCASLGVLGSDSAALLADAGIAHYNINLQV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHCCEEEEEEEEEE
SPDRYSDLIADSHSQNDRIETIRLLRKHRIAVCCGAILGVGETMQERIDLIFVLQALDVA
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHE
VIPLNVLVPVDGTPLEGSLPLAVSDIVKTFALCRLAHPDKVIKFAAGRETVMKDFQGLLM
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHEE
LAGANGFLTGGYLTTRGRDVGDDQAFMEQLKHFSGRS
EECCCCEEECCEEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA