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The map label for this gene is rsmE [H]

Identifier: 194334810

GI number: 194334810

Start: 2209967

End: 2210704

Strand: Reverse

Name: rsmE [H]

Synonym: Paes_2013

Alternate gene names: 194334810

Gene position: 2210704-2209967 (Counterclockwise)

Preceding gene: 194334811

Following gene: 194334809

Centisome position: 87.97

GC content: 51.22

Gene sequence:

>738_bases
ATGGATCTTTTCTACACACCGCCGGAATGTATTGATCTGACGGCACGCACGCTTGTTGTCGAGGGAGAAGAGTTTTTCCA
TATTGTTCGTGTTCTGCGCCGCACAGAGGGCCAGAGTCTTCATCTTACCGATGGCTGCGGCTTGTCGATCAATGCAGTTA
TCGACTCGATCGACAAAGGGCGTCTGAACGCTTCTATTGCATCCTTTCAGCATATTGAACCTCCGGCAACAGAGGTGACG
GTCGCGATATCGCTGTTGAAATCGGCTCAGCGTTTCGACTTTTTTCTTGAAAAGGCGACCGAGCTCGGTGTCACATCAAT
CATTCCTATGGTGACATCCCGTACCGTTTCCCAGCCGCGAGGAGAGAAAGTGCAGCGAAAAGTCGAGCGGTGGAAAAAAG
TATTGATTGCCGCATCGATACAGAGCAAGCGCTACTCTCTTCCCGATATTTGTGAGCCCCGGTGTTTTGGTGAAGTTCTC
TCAGAAGGGGGCAGTTATGATGAACGACTGATCCCCTACGAGTCGTCGTGTCAGTCACCCTCTGTTGCGTTAGCCGGCAG
GAAAACGCTGTTTGTTATTGGCGGTGAAGGCGGATTTAGCGAAACCGAGGTGCAGCAGGCAAAGGACTCCGGGTTTACCG
AGATCTCTTTCGGCTCTTCGATTCTCCGGGCTGAAACTGCCGCAGTATTTGCTGTTGCCATGGCTCGCGCCGATATGCTT
TTGAATAACGATGAATAA

Upstream 100 bases:

>100_bases
TTCGACGGTTGCCGCGGCGATGGATGTGCTTGCTCAATGCGGTGCGCAAAAAATTTCTCTTGCAATCGTGGCTCTTGCCA
CCTCCTGAAAAAAACATGTT

Downstream 100 bases:

>100_bases
TTATCAAGAAGAAAGGAGATCCTGATGAAGAGGGCTGAACTTAATAATCTTGTTCTGCTGGTCTTTGTTCTGCTTATTTC
GGCAATCTTTTTTTCCATGA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDLFYTPPECIDLTARTLVVEGEEFFHIVRVLRRTEGQSLHLTDGCGLSINAVIDSIDKGRLNASIASFQHIEPPATEVT
VAISLLKSAQRFDFFLEKATELGVTSIIPMVTSRTVSQPRGEKVQRKVERWKKVLIAASIQSKRYSLPDICEPRCFGEVL
SEGGSYDERLIPYESSCQSPSVALAGRKTLFVIGGEGGFSETEVQQAKDSGFTEISFGSSILRAETAAVFAVAMARADML
LNNDE

Sequences:

>Translated_245_residues
MDLFYTPPECIDLTARTLVVEGEEFFHIVRVLRRTEGQSLHLTDGCGLSINAVIDSIDKGRLNASIASFQHIEPPATEVT
VAISLLKSAQRFDFFLEKATELGVTSIIPMVTSRTVSQPRGEKVQRKVERWKKVLIAASIQSKRYSLPDICEPRCFGEVL
SEGGSYDERLIPYESSCQSPSVALAGRKTLFVIGGEGGFSETEVQQAKDSGFTEISFGSSILRAETAAVFAVAMARADML
LNNDE
>Mature_245_residues
MDLFYTPPECIDLTARTLVVEGEEFFHIVRVLRRTEGQSLHLTDGCGLSINAVIDSIDKGRLNASIASFQHIEPPATEVT
VAISLLKSAQRFDFFLEKATELGVTSIIPMVTSRTVSQPRGEKVQRKVERWKKVLIAASIQSKRYSLPDICEPRCFGEVL
SEGGSYDERLIPYESSCQSPSVALAGRKTLFVIGGEGGFSETEVQQAKDSGFTEISFGSSILRAETAAVFAVAMARADML
LNNDE

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=210, Percent_Identity=29.0476190476191, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26872; Mature: 26872

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFYTPPECIDLTARTLVVEGEEFFHIVRVLRRTEGQSLHLTDGCGLSINAVIDSIDKG
CCCCCCCHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
RLNASIASFQHIEPPATEVTVAISLLKSAQRFDFFLEKATELGVTSIIPMVTSRTVSQPR
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GEKVQRKVERWKKVLIAASIQSKRYSLPDICEPRCFGEVLSEGGSYDERLIPYESSCQSP
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCC
SVALAGRKTLFVIGGEGGFSETEVQQAKDSGFTEISFGSSILRAETAAVFAVAMARADML
CEEEECCEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LNNDE
CCCCC
>Mature Secondary Structure
MDLFYTPPECIDLTARTLVVEGEEFFHIVRVLRRTEGQSLHLTDGCGLSINAVIDSIDKG
CCCCCCCHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
RLNASIASFQHIEPPATEVTVAISLLKSAQRFDFFLEKATELGVTSIIPMVTSRTVSQPR
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GEKVQRKVERWKKVLIAASIQSKRYSLPDICEPRCFGEVLSEGGSYDERLIPYESSCQSP
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCC
SVALAGRKTLFVIGGEGGFSETEVQQAKDSGFTEISFGSSILRAETAAVFAVAMARADML
CEEEECCEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LNNDE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9729120 [H]