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The map label for this gene is cysK [H]

Identifier: 194334800

GI number: 194334800

Start: 2200515

End: 2201441

Strand: Reverse

Name: cysK [H]

Synonym: Paes_2003

Alternate gene names: 194334800

Gene position: 2201441-2200515 (Counterclockwise)

Preceding gene: 194334801

Following gene: 194334799

Centisome position: 87.6

GC content: 53.83

Gene sequence:

>927_bases
ATGAATGTCTACGAGAGCATAACAGAGACGGTCGGTCGCACACCTCTTGTGCGCATTCGTCGTATGGCGGATCATTCGTA
TGCTGATGTCTATGCCAAGCTGGAATCCTTCAATCCGCTTTCAAGCGTCAAAGACCGCGTCGGTGTCGCCATGATCGAGG
ATGCGGAACGAAACGGCCTGATAGGCCCGGAGACGACCATTATTGAGCCGACCAGCGGCAATACGGGAATAGCGCTTGCG
TTTAGCTGTGCATCGAGGGGATACCGGCTTATTCTCACCATGCCGGATACCATGAGCGTCGAACGTCGCAGGCTTCTGAA
AATTCTGGGAGCCGAACTTGTTCTGACGGAAGGTGCAGCAGGAATGAAAGGCGCCATAGCGGAAGCTGAGCGGCTTGTCG
ATTCGATTCCTCACAGCCTGATGCTGCAGCAGTTCAATAACCCTGCCAACCCGGAGACGCACCGCAGGACAACAGCGAGA
GAAATATGGGACGATACCGACGGTCAGGTTGATATATTTATTGCCGGAGTTGGAACGGGCGGTACTATTACCGGTGTCGG
CAGCGTACTCAGGGAGCTGAAACCAGGGGTTCATATTGTGGCTGTCGAGCCAGACGATTCGCCGGTTATTTCCGGAGGCG
AACCCGGGCCGCATAAAATTCAGGGGATCGGAGCCGGTTTTGTTCCTGGAAATCTTGACCCTTCGGTTATAGACGAGATC
ATAACCGTCTCAAATCAGGATGCCGCCGAGACAGCAAGAAAGCTTGCCCGCAAAGAGGGTATCCTTTGCGGGATCTCTTC
GGGAGCCGCCATGTGGGCGGCAATGAGCGTTGCTGCCCGACCTGCTATGGAGGGTAAAAATATTGTTGTACTTTTGCCTG
ATACAGGGGAGCGCTATCTTTCAACCTGGTTGTTTGCCGATGAGTAA

Upstream 100 bases:

>100_bases
AGGTGCCTATATGATTATATTCCGGCATTGATCATTCGTGTGCTGCGGTCACGTGTTCGGCTGCAGAAAGAGTTAACTTG
AACGTTTCAGGATTTGTATC

Downstream 100 bases:

>100_bases
GCACTCCGCTGCACCCGGGTATTCTGCAAAACAAAGAAGTTGAGAACAGATTATGGAAACACGACGGGTCTATCTGGACC
ATAATGCCACAACCCCGCTA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGLIGPETTIIEPTSGNTGIALA
FSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAAGMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAR
EIWDDTDGQVDIFIAGVGTGGTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI
ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYLSTWLFADE

Sequences:

>Translated_308_residues
MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGLIGPETTIIEPTSGNTGIALA
FSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAAGMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAR
EIWDDTDGQVDIFIAGVGTGGTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI
ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYLSTWLFADE
>Mature_308_residues
MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGLIGPETTIIEPTSGNTGIALA
FSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAAGMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAR
EIWDDTDGQVDIFIAGVGTGGTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI
ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYLSTWLFADE

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=41.5335463258786, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=41.5335463258786, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=41.5335463258786, Blast_Score=232, Evalue=4e-61,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=58.4126984126984, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=42.9042904290429, Blast_Score=234, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=51.3333333333333, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=50.6666666666667, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=51.1945392491468, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=52.6315789473684, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=48.1605351170569, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=52.803738317757, Blast_Score=223, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=39.375, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=317, Percent_Identity=37.8548895899054, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=47.8632478632479, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=37.6175548589342, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321449, Length=336, Percent_Identity=35.4166666666667, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=39.8058252427184, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=39.8058252427184, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32877; Mature: 32877

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGL
CCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCEEEEECCCCCCC
IGPETTIIEPTSGNTGIALAFSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAA
CCCCCEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCC
GMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAREIWDDTDGQVDIFIAGVGTG
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCC
GTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI
CCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCCCCCCCCHHHHHHH
ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYL
HHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
STWLFADE
HHEEECCC
>Mature Secondary Structure
MNVYESITETVGRTPLVRIRRMADHSYADVYAKLESFNPLSSVKDRVGVAMIEDAERNGL
CCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCEEEEECCCCCCC
IGPETTIIEPTSGNTGIALAFSCASRGYRLILTMPDTMSVERRRLLKILGAELVLTEGAA
CCCCCEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCC
GMKGAIAEAERLVDSIPHSLMLQQFNNPANPETHRRTTAREIWDDTDGQVDIFIAGVGTG
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCC
GTITGVGSVLRELKPGVHIVAVEPDDSPVISGGEPGPHKIQGIGAGFVPGNLDPSVIDEI
CCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCCCCCCCCHHHHHHH
ITVSNQDAAETARKLARKEGILCGISSGAAMWAAMSVAARPAMEGKNIVVLLPDTGERYL
HHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
STWLFADE
HHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]