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The map label for this gene is iscS [H]

Identifier: 194334799

GI number: 194334799

Start: 2199257

End: 2200462

Strand: Reverse

Name: iscS [H]

Synonym: Paes_2002

Alternate gene names: 194334799

Gene position: 2200462-2199257 (Counterclockwise)

Preceding gene: 194334800

Following gene: 194334798

Centisome position: 87.57

GC content: 51.66

Gene sequence:

>1206_bases
ATGGAAACACGACGGGTCTATCTGGACCATAATGCCACAACCCCGCTACATCCCGAGGTTAAAAAAGAGATGATCGAAGC
GATGGAAATGTTCGGTAATCCATCGAGCATGCATGCTTTCGGGCGTGAAGCACATGCCAATATCGAACATGCGCGTCAAA
GCGTTGCTGGATTTATCGGTGCGCATGAAGACGAACTTGTTTTTGTCGGCAGTGGTTCCGAGGCTAACAATACCGTTCTC
TCACTGTTTGCCTGTGCGTCAAATCTTTGTATTCCGGGATTTAAAGCCCGTCAGACAATCATTACAAGCCGGATCGAGCA
TCCATGCGTGCTTGAAACCTCTCAGTGTCTTGCTCACCGCGGTACCACGGTGAAGCTGCTCGATGTGGACCGCTACGGGC
GCATCGATATCGACCAGCTCAAAGAGTATCTTTCCGATGATGTGGGGCTTGTTTCGGTGATGACGGCAAACAACGAGATC
GGCACGCTCCAGGATATTTCGGAAATCGGTCGTCTGGCCCATGAAAACGGCTCTCTTATGCACACCGACGCTGTACAGGC
ATTTGGAAAGATGCCGCTCGATGTCGATGAGCTCGGTGTCGATTTTCTGACGATGAGTGCGCATAAAATTTACGGCCCCA
AGGGTATCGGGGCGCTCTATGTTCGTAAAGGCACGCCCTACTGCCCCTTTATCAGGGGAGGTCATCAGGAGAAGGGGCGA
AGGGCCGGCACTGAAAATACGTTAGGGATTATGGGGCTTGCCAAAGCTGTTGAGATGAGGGCGCTCGAGATGGAGGACGA
AGCCAGGCGTTTTGCCGCTATGAAGGAGGTGCTTGTCAAAGGGATCGAGGAGCGAATCGATAACGCGTTATTCAACGGTC
ATCCTGAGCTCAGCATGCCCAATACGGTCAATGTCTCGTTTCCCGGTGCGGAAGGCGAAGCAATTCTGCTCTATCTCGAT
CTTGCCGGAATAGCGGTTTCTACCGGTTCAGCCTGTGCGTCGGGATCGCTCGATCCTTCTCATGTTCTGCTTGCTACCGG
TTCGAGTGCAGAACGGGCCCATGGATCGATCAGGATCAGTATGGGAAGAGAGACGACCATGGAGGAGATCGAGTATGTGC
TCGATGTTCTGCCAGGGGTCATATCACGAATAAGAAGTATGTCAACAGCTTATATAAAAGGAGAAGAACATGTTGCAGCA
AAATGA

Upstream 100 bases:

>100_bases
GATACAGGGGAGCGCTATCTTTCAACCTGGTTGTTTGCCGATGAGTAAGCACTCCGCTGCACCCGGGTATTCTGCAAAAC
AAAGAAGTTGAGAACAGATT

Downstream 100 bases:

>100_bases
CTGGGTATATACCGAGACACTCAAAGAGCATTTCATGAACCCGAAAAATATTCTCCAGGGAGAGGATACCGACGCATTTG
ACGGTGTCGGTATGGAGGGC

Product: aminotransferase class V protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
METRRVYLDHNATTPLHPEVKKEMIEAMEMFGNPSSMHAFGREAHANIEHARQSVAGFIGAHEDELVFVGSGSEANNTVL
SLFACASNLCIPGFKARQTIITSRIEHPCVLETSQCLAHRGTTVKLLDVDRYGRIDIDQLKEYLSDDVGLVSVMTANNEI
GTLQDISEIGRLAHENGSLMHTDAVQAFGKMPLDVDELGVDFLTMSAHKIYGPKGIGALYVRKGTPYCPFIRGGHQEKGR
RAGTENTLGIMGLAKAVEMRALEMEDEARRFAAMKEVLVKGIEERIDNALFNGHPELSMPNTVNVSFPGAEGEAILLYLD
LAGIAVSTGSACASGSLDPSHVLLATGSSAERAHGSIRISMGRETTMEEIEYVLDVLPGVISRIRSMSTAYIKGEEHVAA
K

Sequences:

>Translated_401_residues
METRRVYLDHNATTPLHPEVKKEMIEAMEMFGNPSSMHAFGREAHANIEHARQSVAGFIGAHEDELVFVGSGSEANNTVL
SLFACASNLCIPGFKARQTIITSRIEHPCVLETSQCLAHRGTTVKLLDVDRYGRIDIDQLKEYLSDDVGLVSVMTANNEI
GTLQDISEIGRLAHENGSLMHTDAVQAFGKMPLDVDELGVDFLTMSAHKIYGPKGIGALYVRKGTPYCPFIRGGHQEKGR
RAGTENTLGIMGLAKAVEMRALEMEDEARRFAAMKEVLVKGIEERIDNALFNGHPELSMPNTVNVSFPGAEGEAILLYLD
LAGIAVSTGSACASGSLDPSHVLLATGSSAERAHGSIRISMGRETTMEEIEYVLDVLPGVISRIRSMSTAYIKGEEHVAA
K
>Mature_401_residues
METRRVYLDHNATTPLHPEVKKEMIEAMEMFGNPSSMHAFGREAHANIEHARQSVAGFIGAHEDELVFVGSGSEANNTVL
SLFACASNLCIPGFKARQTIITSRIEHPCVLETSQCLAHRGTTVKLLDVDRYGRIDIDQLKEYLSDDVGLVSVMTANNEI
GTLQDISEIGRLAHENGSLMHTDAVQAFGKMPLDVDELGVDFLTMSAHKIYGPKGIGALYVRKGTPYCPFIRGGHQEKGR
RAGTENTLGIMGLAKAVEMRALEMEDEARRFAAMKEVLVKGIEERIDNALFNGHPELSMPNTVNVSFPGAEGEAILLYLD
LAGIAVSTGSACASGSLDPSHVLLATGSSAERAHGSIRISMGRETTMEEIEYVLDVLPGVISRIRSMSTAYIKGEEHVAA
K

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=397, Percent_Identity=40.5541561712846, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=35.3080568720379, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI48994898, Length=396, Percent_Identity=41.4141414141414, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1787970, Length=319, Percent_Identity=28.5266457680251, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789175, Length=299, Percent_Identity=28.7625418060201, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=39.0862944162437, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=32.9230769230769, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=396, Percent_Identity=40.6565656565657, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI20129463, Length=395, Percent_Identity=40.253164556962, Blast_Score=278, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43510; Mature: 43510

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRRVYLDHNATTPLHPEVKKEMIEAMEMFGNPSSMHAFGREAHANIEHARQSVAGFIG
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHCCHHHHHHHHHHHHC
AHEDELVFVGSGSEANNTVLSLFACASNLCIPGFKARQTIITSRIEHPCVLETSQCLAHR
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHC
GTTVKLLDVDRYGRIDIDQLKEYLSDDVGLVSVMTANNEIGTLQDISEIGRLAHENGSLM
CCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
HTDAVQAFGKMPLDVDELGVDFLTMSAHKIYGPKGIGALYVRKGTPYCPFIRGGHQEKGR
EHHHHHHHCCCCCCHHHHCCCEEEECHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHC
RAGTENTLGIMGLAKAVEMRALEMEDEARRFAAMKEVLVKGIEERIDNALFNGHPELSMP
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTVNVSFPGAEGEAILLYLDLAGIAVSTGSACASGSLDPSHVLLATGSSAERAHGSIRIS
CEEEEEECCCCCCEEEEEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE
MGRETTMEEIEYVLDVLPGVISRIRSMSTAYIKGEEHVAAK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCC
>Mature Secondary Structure
METRRVYLDHNATTPLHPEVKKEMIEAMEMFGNPSSMHAFGREAHANIEHARQSVAGFIG
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCHHHHCCHHHHHHHHHHHHC
AHEDELVFVGSGSEANNTVLSLFACASNLCIPGFKARQTIITSRIEHPCVLETSQCLAHR
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHC
GTTVKLLDVDRYGRIDIDQLKEYLSDDVGLVSVMTANNEIGTLQDISEIGRLAHENGSLM
CCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
HTDAVQAFGKMPLDVDELGVDFLTMSAHKIYGPKGIGALYVRKGTPYCPFIRGGHQEKGR
EHHHHHHHCCCCCCHHHHCCCEEEECHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHC
RAGTENTLGIMGLAKAVEMRALEMEDEARRFAAMKEVLVKGIEERIDNALFNGHPELSMP
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTVNVSFPGAEGEAILLYLDLAGIAVSTGSACASGSLDPSHVLLATGSSAERAHGSIRIS
CEEEEEECCCCCCEEEEEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE
MGRETTMEEIEYVLDVLPGVISRIRSMSTAYIKGEEHVAAK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA