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The map label for this gene is dus1 [H]

Identifier: 194334749

GI number: 194334749

Start: 2131469

End: 2132512

Strand: Reverse

Name: dus1 [H]

Synonym: Paes_1950

Alternate gene names: 194334749

Gene position: 2132512-2131469 (Counterclockwise)

Preceding gene: 194334750

Following gene: 194334748

Centisome position: 84.86

GC content: 53.54

Gene sequence:

>1044_bases
ATGAAGGTCGGGTCATTGGACATCGGTCAGGCGATTATGCTCGCGCCTATGGAAGAGGTGACTGATCGTTCTTTCAGGAA
AATCTGTAAGCGGTTCGGGGCAGATGTTGTCTATACTGAATTTATCAGTGCCGAAGCGATCCGTCGACAGGTCGATACGT
CGCTCCGTAAAATGCGGTTTGACGATGATGAGGCTCCTGTTGTCGTTCAGATTTTCGGCAACAGCCAGGAAGCTATGGCT
GAGGCTGCTGTCATTGCGGCATCGTGCAGCCCGGCATGGATCGATATCAATTTTGGTTGTCCCGCAAAAAAAGTTGCCGG
GAAAGGGGCCGGTGCTGCCCTGCTGAAAGAACCCGATAAAATGGTCGCGATTGCCGCTGCGGTTGTCCGTGCGGTCAATC
TGCCTGTTACGGTCAAAACGCGTCTTGGCTGGGATCGCGATTCAATCAATATTGTCGATATCGTGCCCCGTCTGGAGGAT
GTCGGGGTTCAGGCTCTGGCGATTCACGGGCGGACGCGCAGCGAGATGTACAGGGGCGTGGCCGATTGGGAGTGGATCCG
AAAGGTGACGGAGAAGGCCAGAATACCGGTTATCGCCAATGGCGATATATGGAGTGCAGCGGATGCCAGGGCGATGTTTG
CCGAGACCGGGGCTGATGCTGTCATGATAGGACGAGGAGCGATTGGTAATCCTTTTATCTTCGCTCAGGCCAGGGAGCTT
CTCGATACGGGGCGCGTGGTGACGCATCCTGATTACAGGGATCGCATTGCCGTTGCGGTCGAACATCTGAAACTGTCGGT
TGAATTCAAGGGGGAGAAGTACGGAAGCCTTGAAATGCGGAGGCATTACTCAACCTATCTGAAAGGACTTCCGCGGGTTT
CCAGGGTTCGCGACAAGCTGGTTCGCGAACCGGACTGGAGAAACGTCATCGAAATCCTTCAGGCTTACGAGGTCGAATGC
GAAGGCTATGAACGCGAAGGAAAGATCCGCGATTATGCGGAATTTCTCAATGACCATTCGAAGCGATTGACATTAAACTA
TTAG

Upstream 100 bases:

>100_bases
AAAGCCTTCAGGGAGGATCAGGAGCTTTACAGCCGTATTCAGCAGCAGGTCAAAGAGGTGATGTCGCCTGCGGTTCCTGT
GGAAAAGACGGATAGTTAAT

Downstream 100 bases:

>100_bases
AAGAAAACAGACGATATTTATGAGCAGTACACAGAAAGGATACCGCGTTGCCATTCTGGGAGCGACCGGTCTTGTCGGCA
GGACGATGCTGGACGTTCTT

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKVGSLDIGQAIMLAPMEEVTDRSFRKICKRFGADVVYTEFISAEAIRRQVDTSLRKMRFDDDEAPVVVQIFGNSQEAMA
EAAVIAASCSPAWIDINFGCPAKKVAGKGAGAALLKEPDKMVAIAAAVVRAVNLPVTVKTRLGWDRDSINIVDIVPRLED
VGVQALAIHGRTRSEMYRGVADWEWIRKVTEKARIPVIANGDIWSAADARAMFAETGADAVMIGRGAIGNPFIFAQAREL
LDTGRVVTHPDYRDRIAVAVEHLKLSVEFKGEKYGSLEMRRHYSTYLKGLPRVSRVRDKLVREPDWRNVIEILQAYEVEC
EGYEREGKIRDYAEFLNDHSKRLTLNY

Sequences:

>Translated_347_residues
MKVGSLDIGQAIMLAPMEEVTDRSFRKICKRFGADVVYTEFISAEAIRRQVDTSLRKMRFDDDEAPVVVQIFGNSQEAMA
EAAVIAASCSPAWIDINFGCPAKKVAGKGAGAALLKEPDKMVAIAAAVVRAVNLPVTVKTRLGWDRDSINIVDIVPRLED
VGVQALAIHGRTRSEMYRGVADWEWIRKVTEKARIPVIANGDIWSAADARAMFAETGADAVMIGRGAIGNPFIFAQAREL
LDTGRVVTHPDYRDRIAVAVEHLKLSVEFKGEKYGSLEMRRHYSTYLKGLPRVSRVRDKLVREPDWRNVIEILQAYEVEC
EGYEREGKIRDYAEFLNDHSKRLTLNY
>Mature_347_residues
MKVGSLDIGQAIMLAPMEEVTDRSFRKICKRFGADVVYTEFISAEAIRRQVDTSLRKMRFDDDEAPVVVQIFGNSQEAMA
EAAVIAASCSPAWIDINFGCPAKKVAGKGAGAALLKEPDKMVAIAAAVVRAVNLPVTVKTRLGWDRDSINIVDIVPRLED
VGVQALAIHGRTRSEMYRGVADWEWIRKVTEKARIPVIANGDIWSAADARAMFAETGADAVMIGRGAIGNPFIFAQAREL
LDTGRVVTHPDYRDRIAVAVEHLKLSVEFKGEKYGSLEMRRHYSTYLKGLPRVSRVRDKLVREPDWRNVIEILQAYEVEC
EGYEREGKIRDYAEFLNDHSKRLTLNY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=34.0336134453782, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=34.0336134453782, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=33.2, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=32.1888412017167, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1789660, Length=316, Percent_Identity=39.2405063291139, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=36.5217391304348, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI145693211, Length=235, Percent_Identity=28.936170212766, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=240, Percent_Identity=32.5, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=30.2325581395349, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=246, Percent_Identity=29.2682926829268, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=268, Percent_Identity=30.5970149253731, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=28.8793103448276, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323433, Length=317, Percent_Identity=25.5520504731861, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=32.0833333333333, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=32.15859030837, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=32.15859030837, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=33.1983805668016, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=28.4444444444444, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38831; Mature: 38831

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGSLDIGQAIMLAPMEEVTDRSFRKICKRFGADVVYTEFISAEAIRRQVDTSLRKMRF
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCC
DDDEAPVVVQIFGNSQEAMAEAAVIAASCSPAWIDINFGCPAKKVAGKGAGAALLKEPDK
CCCCCCEEEEEECCCHHHHHHHHHHEECCCCEEEEEECCCCHHHHCCCCCCHHHHCCCHH
MVAIAAAVVRAVNLPVTVKTRLGWDRDSINIVDIVPRLEDVGVQALAIHGRTRSEMYRGV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHCCCEEEEECCCCHHHHHCCC
ADWEWIRKVTEKARIPVIANGDIWSAADARAMFAETGADAVMIGRGAIGNPFIFAQAREL
CCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LDTGRVVTHPDYRDRIAVAVEHLKLSVEFKGEKYGSLEMRRHYSTYLKGLPRVSRVRDKL
HHCCCEEECCCHHHHHHHEEEHHEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
VREPDWRNVIEILQAYEVECEGYEREGKIRDYAEFLNDHSKRLTLNY
HCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKVGSLDIGQAIMLAPMEEVTDRSFRKICKRFGADVVYTEFISAEAIRRQVDTSLRKMRF
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCC
DDDEAPVVVQIFGNSQEAMAEAAVIAASCSPAWIDINFGCPAKKVAGKGAGAALLKEPDK
CCCCCCEEEEEECCCHHHHHHHHHHEECCCCEEEEEECCCCHHHHCCCCCCHHHHCCCHH
MVAIAAAVVRAVNLPVTVKTRLGWDRDSINIVDIVPRLEDVGVQALAIHGRTRSEMYRGV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHCCCEEEEECCCCHHHHHCCC
ADWEWIRKVTEKARIPVIANGDIWSAADARAMFAETGADAVMIGRGAIGNPFIFAQAREL
CCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LDTGRVVTHPDYRDRIAVAVEHLKLSVEFKGEKYGSLEMRRHYSTYLKGLPRVSRVRDKL
HHCCCEEECCCHHHHHHHEEEHHEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
VREPDWRNVIEILQAYEVECEGYEREGKIRDYAEFLNDHSKRLTLNY
HCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]