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The map label for this gene is hypF2 [H]

Identifier: 194334448

GI number: 194334448

Start: 1787616

End: 1789952

Strand: Reverse

Name: hypF2 [H]

Synonym: Paes_1643

Alternate gene names: 194334448

Gene position: 1789952-1787616 (Counterclockwise)

Preceding gene: 194334449

Following gene: 194334447

Centisome position: 71.23

GC content: 53.66

Gene sequence:

>2337_bases
ATGGGTGGCGTGGTTGAAAGCAGCAATGAACCGATCCTGAGCGGGAGAGCAAGGCGCCTGCGTCTCGCGGTTAACGGGAT
TGTTCAGGGTGTCGGGTTCCGGCCTTATGTCTACCGCCTTGCGACATCTCTGGGGCTGAGCGGTTCGATTCGCAATACCG
CTTCCGGCGTTTTGATCGAAGTGCAGGCTGAGACTCCGTTGCTTCTGGAGCGCTTCGTTCAGGAGCTTGGTTCTGAAGCT
CCTCCTCTCGCAGAAATTTTTTCCGTTGTTGCCGATGAGATTCCCGCTCAATCGGGAAACGGATTTGTGATTCTCTCTTC
AGATGACGGTGAAGATGTCGAAACCCTGATTCCTCCTGATATCGCGCTGTGCGATGATTGTCGTTCAGAGCTTCTTGATC
CTCTTGATCGACGCTACCGCTATGCGTTTATCAATTGTACGAATTGCGGGCCGCGCTTCAGTATTGTTGAGCGTCTTCCA
TACGACCGTCCTTTGACATCGATGAAGGGATTCACGATGTGCGAGAAGTGCGGTCGGGAGTACGGTAATCCTCTCGATCG
TCGTTTTCACGCGCAGCCGAACGCCTGTCCGGATTGCGGGCCGGCCCTTCAGTTACTTGATGCAGGCGGAGAGCCGATCA
ATGCGCCTGATCCTCTGTCGCTCGCACTCCAGAAGCTGAAAGATGGCGATATTGTCGCAGTCAAGGGGGTGGGGGGATTT
CATCTCGCTGTTGACGCAACAGATCAGTCTGCCGTGATGAATCTGCGAGAGAGAAAAGGGAGGGAGCGAAAGCCTTTCGC
CGTGATGGCACGATCATTGGGCTCTGCTGAAAAAGTGGCTCAGGTATCGGATGAAGAACGTGTTGCGCTGCGCTCTTTGC
AGGCTCCGGTTGTGCTGATGCATAAGAAAGCATCCGCCTCGTTGCTGGCTCCTGACGTGGCTCCTGCTAACGATCGGATC
GGCCTCATGCTTCCCTATTCTCCTCTTCATGTTCTTATGATGGAGGAGGGCCCGGAATTTCTGGTGATGACGAGTGCCAA
CTCCAGCGAGGAGCCTATTGCTCTTGAAAACGATGAGGCGGTCAGTCGATTGCAGGGGATTGCAGATTATTTTCTTGTCC
ATAACCGGCCAATACATCTTCGATGCGATGATTCCGTCACGATGGTGATGTCAGGGGCGTTGCGACAGATCAGACGGGGT
CGCGGTTATGCCCCGCTGCCTGTTCTGCTTTCTTCGGATGGGCCTTCAGTGCTTGCGGCCGGGGGTGAAATGAAGAATAC
GGTTTGTGTGCTTCAAGGATCTCAGGCTCTTTTGAGCCAGCATATCGGCGATCTGAAAAATTATGTGGCATATGAGCATT
TTCAGCAAGTTGCTGAGCATATGCAGCATATTTTTCAGGTTCGTCCCGAAGCGATTATCAGTGATATGCATCCATCATAT
CTTTCGACGCAATGGGCTCAAAACCAGAGTGATATCCCCGTTCTTCATGTTCAGCATCATCATGCTCATCTGGTATCCTG
TCTTGCGGAAAACCGTTTTGACGGCCAGGCTGTCGGTATCATCCTTGATGGGACAGGATATGGCACCGATGGTACAGTCT
GGGGCGGAGAGGTGCTTATCGGAGACGCATCGGGGTTCTTTCGTTTCGCGTCACTTGAACCGGTCCGGATGCCCGGAGGT
GATCGTGCAGCGCTTTTTCCGTGGAAAGCCGCTGCGGGATACCTGTTTCATACTTATGGTCATATTCCGGAGATAGCGGC
TTTTGAGGGGTGCTTTGTCGAGGGTATTGCCGATCTTCTCGCCAGACAGGTCAATGCACCTCCTGCCAGCAGTTGCGGCC
GTCTTTTTGATGCGGTTTCAGCCCTGTGCGGGTTGTGCAGAGAAATCAGTTATGAGGGGCAGGCGGCTATCGAACTGATG
CATGCTGCAGGCATTGCCGAGGGGAAACCCTTTGCCTGGGAGGTCGTTTCTGCTGGTTCAGATCGGTGGCATCTGTCTGT
TGCACCGATGGTACAGGATATTGTGACGGCACTCGGTTCCGCAATGAGTGTTTCGGACATCAGTCGACGATTTCATGTCA
CGATTGTTAAAATGTTTTCTGATATTGCTCTCAGGGCCTGTCGTTTTTCCGGCCTTACTTCGGTTGCCCTCAGCGGGGGA
GTTTTTCAGAATCCGCTGGTGTTCGAAGGGCTTGTCAGCGATCTTCAGCTGCACGGGATTGAGGTGCTGACCCATAGCCA
GGTTCCATCGAACGATGGAGGCCTTTCACTCGGTCAGGCCGTGATCGGAAGGCATTGGGTGAAGACGGGATGCGCCTGTC
GGCGCAGTGACGCGTGA

Upstream 100 bases:

>100_bases
TTCCCTTTGATGCTGTGCAATGCCGGGAAAATGCGATGAACGTCAATCATCATCTTGCCTGGTTTGAGCTTTCAGCTTCA
ACAGGTGAAGGAGTTGACGC

Downstream 100 bases:

>100_bases
CGAGTGACGAGGGGCGCTTTGCGCAAGAGAAGAGATAGAGGAGGTTGGAGATCGGGTTTCGTTCGGTGCTTCTATTGTCG
GCAGACTATACTGATGGGCA

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF2; [NiFe]-hydrogenase maturation factor hypF2; Hydrogenase maturation protein hypF2 [H]

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MGGVVESSNEPILSGRARRLRLAVNGIVQGVGFRPYVYRLATSLGLSGSIRNTASGVLIEVQAETPLLLERFVQELGSEA
PPLAEIFSVVADEIPAQSGNGFVILSSDDGEDVETLIPPDIALCDDCRSELLDPLDRRYRYAFINCTNCGPRFSIVERLP
YDRPLTSMKGFTMCEKCGREYGNPLDRRFHAQPNACPDCGPALQLLDAGGEPINAPDPLSLALQKLKDGDIVAVKGVGGF
HLAVDATDQSAVMNLRERKGRERKPFAVMARSLGSAEKVAQVSDEERVALRSLQAPVVLMHKKASASLLAPDVAPANDRI
GLMLPYSPLHVLMMEEGPEFLVMTSANSSEEPIALENDEAVSRLQGIADYFLVHNRPIHLRCDDSVTMVMSGALRQIRRG
RGYAPLPVLLSSDGPSVLAAGGEMKNTVCVLQGSQALLSQHIGDLKNYVAYEHFQQVAEHMQHIFQVRPEAIISDMHPSY
LSTQWAQNQSDIPVLHVQHHHAHLVSCLAENRFDGQAVGIILDGTGYGTDGTVWGGEVLIGDASGFFRFASLEPVRMPGG
DRAALFPWKAAAGYLFHTYGHIPEIAAFEGCFVEGIADLLARQVNAPPASSCGRLFDAVSALCGLCREISYEGQAAIELM
HAAGIAEGKPFAWEVVSAGSDRWHLSVAPMVQDIVTALGSAMSVSDISRRFHVTIVKMFSDIALRACRFSGLTSVALSGG
VFQNPLVFEGLVSDLQLHGIEVLTHSQVPSNDGGLSLGQAVIGRHWVKTGCACRRSDA

Sequences:

>Translated_778_residues
MGGVVESSNEPILSGRARRLRLAVNGIVQGVGFRPYVYRLATSLGLSGSIRNTASGVLIEVQAETPLLLERFVQELGSEA
PPLAEIFSVVADEIPAQSGNGFVILSSDDGEDVETLIPPDIALCDDCRSELLDPLDRRYRYAFINCTNCGPRFSIVERLP
YDRPLTSMKGFTMCEKCGREYGNPLDRRFHAQPNACPDCGPALQLLDAGGEPINAPDPLSLALQKLKDGDIVAVKGVGGF
HLAVDATDQSAVMNLRERKGRERKPFAVMARSLGSAEKVAQVSDEERVALRSLQAPVVLMHKKASASLLAPDVAPANDRI
GLMLPYSPLHVLMMEEGPEFLVMTSANSSEEPIALENDEAVSRLQGIADYFLVHNRPIHLRCDDSVTMVMSGALRQIRRG
RGYAPLPVLLSSDGPSVLAAGGEMKNTVCVLQGSQALLSQHIGDLKNYVAYEHFQQVAEHMQHIFQVRPEAIISDMHPSY
LSTQWAQNQSDIPVLHVQHHHAHLVSCLAENRFDGQAVGIILDGTGYGTDGTVWGGEVLIGDASGFFRFASLEPVRMPGG
DRAALFPWKAAAGYLFHTYGHIPEIAAFEGCFVEGIADLLARQVNAPPASSCGRLFDAVSALCGLCREISYEGQAAIELM
HAAGIAEGKPFAWEVVSAGSDRWHLSVAPMVQDIVTALGSAMSVSDISRRFHVTIVKMFSDIALRACRFSGLTSVALSGG
VFQNPLVFEGLVSDLQLHGIEVLTHSQVPSNDGGLSLGQAVIGRHWVKTGCACRRSDA
>Mature_777_residues
GGVVESSNEPILSGRARRLRLAVNGIVQGVGFRPYVYRLATSLGLSGSIRNTASGVLIEVQAETPLLLERFVQELGSEAP
PLAEIFSVVADEIPAQSGNGFVILSSDDGEDVETLIPPDIALCDDCRSELLDPLDRRYRYAFINCTNCGPRFSIVERLPY
DRPLTSMKGFTMCEKCGREYGNPLDRRFHAQPNACPDCGPALQLLDAGGEPINAPDPLSLALQKLKDGDIVAVKGVGGFH
LAVDATDQSAVMNLRERKGRERKPFAVMARSLGSAEKVAQVSDEERVALRSLQAPVVLMHKKASASLLAPDVAPANDRIG
LMLPYSPLHVLMMEEGPEFLVMTSANSSEEPIALENDEAVSRLQGIADYFLVHNRPIHLRCDDSVTMVMSGALRQIRRGR
GYAPLPVLLSSDGPSVLAAGGEMKNTVCVLQGSQALLSQHIGDLKNYVAYEHFQQVAEHMQHIFQVRPEAIISDMHPSYL
STQWAQNQSDIPVLHVQHHHAHLVSCLAENRFDGQAVGIILDGTGYGTDGTVWGGEVLIGDASGFFRFASLEPVRMPGGD
RAALFPWKAAAGYLFHTYGHIPEIAAFEGCFVEGIADLLARQVNAPPASSCGRLFDAVSALCGLCREISYEGQAAIELMH
AAGIAEGKPFAWEVVSAGSDRWHLSVAPMVQDIVTALGSAMSVSDISRRFHVTIVKMFSDIALRACRFSGLTSVALSGGV
FQNPLVFEGLVSDLQLHGIEVLTHSQVPSNDGGLSLGQAVIGRHWVKTGCACRRSDA

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=754, Percent_Identity=40.7161803713528, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83932; Mature: 83801

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGVVESSNEPILSGRARRLRLAVNGIVQGVGFRPYVYRLATSLGLSGSIRNTASGVLIE
CCCCCCCCCCCCCCCCHHEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEE
VQAETPLLLERFVQELGSEAPPLAEIFSVVADEIPAQSGNGFVILSSDDGEDVETLIPPD
EECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCCC
IALCDDCRSELLDPLDRRYRYAFINCTNCGPRFSIVERLPYDRPLTSMKGFTMCEKCGRE
HHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHCCCCCCCHHHCCHHHHHHHHHH
YGNPLDRRFHAQPNACPDCGPALQLLDAGGEPINAPDPLSLALQKLKDGDIVAVKGVGGF
HCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCE
HLAVDATDQSAVMNLRERKGRERKPFAVMARSLGSAEKVAQVSDEERVALRSLQAPVVLM
EEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCEEEE
HKKASASLLAPDVAPANDRIGLMLPYSPLHVLMMEEGPEFLVMTSANSSEEPIALENDEA
ECCCCCCEECCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCEEECCHHH
VSRLQGIADYFLVHNRPIHLRCDDSVTMVMSGALRQIRRGRGYAPLPVLLSSDGPSVLAA
HHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEC
GGEMKNTVCVLQGSQALLSQHIGDLKNYVAYEHFQQVAEHMQHIFQVRPEAIISDMHPSY
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHH
LSTQWAQNQSDIPVLHVQHHHAHLVSCLAENRFDGQAVGIILDGTGYGTDGTVWGGEVLI
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCEEEE
GDASGFFRFASLEPVRMPGGDRAALFPWKAAAGYLFHTYGHIPEIAAFEGCFVEGIADLL
ECCCCCEEEECCCCEECCCCCCEEECCHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARQVNAPPASSCGRLFDAVSALCGLCREISYEGQAAIELMHAAGIAEGKPFAWEVVSAGS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
DRWHLSVAPMVQDIVTALGSAMSVSDISRRFHVTIVKMFSDIALRACRFSGLTSVALSGG
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
VFQNPLVFEGLVSDLQLHGIEVLTHSQVPSNDGGLSLGQAVIGRHWVKTGCACRRSDA
CCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GGVVESSNEPILSGRARRLRLAVNGIVQGVGFRPYVYRLATSLGLSGSIRNTASGVLIE
CCCCCCCCCCCCCCCHHEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEE
VQAETPLLLERFVQELGSEAPPLAEIFSVVADEIPAQSGNGFVILSSDDGEDVETLIPPD
EECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCCC
IALCDDCRSELLDPLDRRYRYAFINCTNCGPRFSIVERLPYDRPLTSMKGFTMCEKCGRE
HHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHCCCCCCCHHHCCHHHHHHHHHH
YGNPLDRRFHAQPNACPDCGPALQLLDAGGEPINAPDPLSLALQKLKDGDIVAVKGVGGF
HCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCE
HLAVDATDQSAVMNLRERKGRERKPFAVMARSLGSAEKVAQVSDEERVALRSLQAPVVLM
EEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCEEEE
HKKASASLLAPDVAPANDRIGLMLPYSPLHVLMMEEGPEFLVMTSANSSEEPIALENDEA
ECCCCCCEECCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCCCCEEECCHHH
VSRLQGIADYFLVHNRPIHLRCDDSVTMVMSGALRQIRRGRGYAPLPVLLSSDGPSVLAA
HHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEC
GGEMKNTVCVLQGSQALLSQHIGDLKNYVAYEHFQQVAEHMQHIFQVRPEAIISDMHPSY
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHH
LSTQWAQNQSDIPVLHVQHHHAHLVSCLAENRFDGQAVGIILDGTGYGTDGTVWGGEVLI
HHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCEEEE
GDASGFFRFASLEPVRMPGGDRAALFPWKAAAGYLFHTYGHIPEIAAFEGCFVEGIADLL
ECCCCCEEEECCCCEECCCCCCEEECCHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARQVNAPPASSCGRLFDAVSALCGLCREISYEGQAAIELMHAAGIAEGKPFAWEVVSAGS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
DRWHLSVAPMVQDIVTALGSAMSVSDISRRFHVTIVKMFSDIALRACRFSGLTSVALSGG
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
VFQNPLVFEGLVSDLQLHGIEVLTHSQVPSNDGGLSLGQAVIGRHWVKTGCACRRSDA
CCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9799289; 12948488 [H]