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The map label for this gene is yafV [C]

Identifier: 194334413

GI number: 194334413

Start: 1752314

End: 1753186

Strand: Reverse

Name: yafV [C]

Synonym: Paes_1608

Alternate gene names: 194334413

Gene position: 1753186-1752314 (Counterclockwise)

Preceding gene: 194334414

Following gene: 194334412

Centisome position: 69.77

GC content: 52.23

Gene sequence:

>873_bases
ATGCGTTCCGATCAAGTGCGAATAGCCCTGGTTCAGATGAGCTGTACTTCAGAGCCTGAAACAAATCTCGATGCCGCCTG
CAGTCGGATCATGGATGCGGCTGAAAAGGGAGCCAGGATCGTCTGTTTGCAGGAGTTGTTCACCTCTCTGTATTTCTGCC
AGGAGGAGTCCTATGAGCCATTCAGTCTCGCTGAACCCGTTCCCGGTCCTTCAACGGGAGTCTTGCAGAAACTTGCGGCT
GAACTGGAAGTTGTTATCGTTGCTTCATTGTTTGAACAGCGTGCGCGTGGTTTGTACCACAATACAGCTGCGGTCATCGA
TGCCGACGGTTCGTACCTCGGGAAATACCGTAAAATGCATATTCCTGATGATCCGGGTTTTTATGAAAAGTTTTATTTCA
CGCCCGGTGATCTTGGCTATCGCGTCTTTAAGACCCGTTATGCAACGATAGGCGTTCTCATCTGCTGGGACCAGTGGTAT
CCTGAAGCAGCCCGTCTGACGGCGCTGAAAGGGGCTGAGATTCTGTTGTATCCTACCGCGATAGGCTGGGCAACAGGTGA
ATCTTCAGCCGAGGTCCGTCGTTCACAGCTTGATGCCTGGCAGACCATACAGCGAAGCCATGCCATCGCCAACGGGGTCT
ATGTCGCTGCGGCAAACAGGGTTGGTGTCGAAGGCGATCTGCAGTTTTGGGGGAGCAGCTTTGTCTGCGATCCTTTTGGT
CAGATGATGACAGTGGGGGACAGTACGGATGAGGCTCTGCTGCTTGCTGACTGCGATCTTTCCGGGATCGGTTTCTACCG
TTCTCATTGGCCGTTTCTTCGTGACAGGCGAATAGAGAGTTATGGCGAGATTGTTCAGAGATATCTGGATTGA

Upstream 100 bases:

>100_bases
CTGAACGATGTCCTTGCCGCTACAGGCAAGGTTATGACCCTCCACGCTCTTTTGCTTTCGGCAGGATTGCTTTTAACAAC
GATTTTAGCGTTTTAACCAT

Downstream 100 bases:

>100_bases
ATAGTATGATCTTCTGCCTTATGCATTGCGATGCGCCATGCCGTTATAGTGAATGAACCACAATGAATATTCATGAGTGT
AACTACTGATGTTAGTATAG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRSDQVRIALVQMSCTSEPETNLDAACSRIMDAAEKGARIVCLQELFTSLYFCQEESYEPFSLAEPVPGPSTGVLQKLAA
ELEVVIVASLFEQRARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYRVFKTRYATIGVLICWDQWY
PEAARLTALKGAEILLYPTAIGWATGESSAEVRRSQLDAWQTIQRSHAIANGVYVAAANRVGVEGDLQFWGSSFVCDPFG
QMMTVGDSTDEALLLADCDLSGIGFYRSHWPFLRDRRIESYGEIVQRYLD

Sequences:

>Translated_290_residues
MRSDQVRIALVQMSCTSEPETNLDAACSRIMDAAEKGARIVCLQELFTSLYFCQEESYEPFSLAEPVPGPSTGVLQKLAA
ELEVVIVASLFEQRARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYRVFKTRYATIGVLICWDQWY
PEAARLTALKGAEILLYPTAIGWATGESSAEVRRSQLDAWQTIQRSHAIANGVYVAAANRVGVEGDLQFWGSSFVCDPFG
QMMTVGDSTDEALLLADCDLSGIGFYRSHWPFLRDRRIESYGEIVQRYLD
>Mature_290_residues
MRSDQVRIALVQMSCTSEPETNLDAACSRIMDAAEKGARIVCLQELFTSLYFCQEESYEPFSLAEPVPGPSTGVLQKLAA
ELEVVIVASLFEQRARGLYHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFTPGDLGYRVFKTRYATIGVLICWDQWY
PEAARLTALKGAEILLYPTAIGWATGESSAEVRRSQLDAWQTIQRSHAIANGVYVAAANRVGVEGDLQFWGSSFVCDPFG
QMMTVGDSTDEALLLADCDLSGIGFYRSHWPFLRDRRIESYGEIVQRYLD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=282, Percent_Identity=34.0425531914894, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI7706509, Length=299, Percent_Identity=32.4414715719064, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI297632348, Length=288, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632350, Length=288, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5031947, Length=288, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI297632346, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=309, Percent_Identity=32.0388349514563, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=284, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=296, Percent_Identity=28.7162162162162, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322335, Length=316, Percent_Identity=25.6329113924051, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358471, Length=313, Percent_Identity=31.9488817891374, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21355835, Length=289, Percent_Identity=26.643598615917, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17933642, Length=289, Percent_Identity=26.9896193771626, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32428; Mature: 32428

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDQVRIALVQMSCTSEPETNLDAACSRIMDAAEKGARIVCLQELFTSLYFCQEESYEP
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCC
FSLAEPVPGPSTGVLQKLAAELEVVIVASLFEQRARGLYHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCEEC
IPDDPGFYEKFYFTPGDLGYRVFKTRYATIGVLICWDQWYPEAARLTALKGAEILLYPTA
CCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCCHHHEEEECCCEEEEEECE
IGWATGESSAEVRRSQLDAWQTIQRSHAIANGVYVAAANRVGVEGDLQFWGSSFVCDPFG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHCCCCCEECCCC
QMMTVGDSTDEALLLADCDLSGIGFYRSHWPFLRDRRIESYGEIVQRYLD
CEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSDQVRIALVQMSCTSEPETNLDAACSRIMDAAEKGARIVCLQELFTSLYFCQEESYEP
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCC
FSLAEPVPGPSTGVLQKLAAELEVVIVASLFEQRARGLYHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCEEC
IPDDPGFYEKFYFTPGDLGYRVFKTRYATIGVLICWDQWYPEAARLTALKGAEILLYPTA
CCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCCHHHEEEECCCEEEEEECE
IGWATGESSAEVRRSQLDAWQTIQRSHAIANGVYVAAANRVGVEGDLQFWGSSFVCDPFG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHCCCCCEECCCC
QMMTVGDSTDEALLLADCDLSGIGFYRSHWPFLRDRRIESYGEIVQRYLD
CEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]