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The map label for this gene is def

Identifier: 194334376

GI number: 194334376

Start: 1710678

End: 1711238

Strand: Reverse

Name: def

Synonym: Paes_1571

Alternate gene names: 194334376

Gene position: 1711238-1710678 (Counterclockwise)

Preceding gene: 194334387

Following gene: 194334375

Centisome position: 68.1

GC content: 48.31

Gene sequence:

>561_bases
ATGATATTACCTATAACAATATACAGTGACGAAGTTCTTCGACGTAAGGCAAAGCCTCTCAAGGGCATTGACACATCTCA
CGAGGAATTGATCGGCAATATGATAGAGTCTATGCGCAATGCATCAGGCATTGGTCTTGCAGCGCCTCAAATAGGCCTTT
CCATGAGGCTTCTCATCGTCGATCTCTCTCCGGTACAGGGGTATGAAAATGCAGAGCCGATGGTGGTGATCAATCCTCAT
ATTCTGGCTGTAAAGGGCTATAATGCTATGGAAGAGGGGTGCTTGAGTATTCCTGATATTCATGCCGATGTCGTCAGGCC
TTCGTCGATTCAGCTTAAGTACCGTAATGAGCATTTCGAGGAGAGAGTAGATGAGTTTTCCGCTTTGATGGCCAGGGTGC
TTCAGCATGAAATCGATCATCTCGATGGCACTCTTTTCGTTGATAAGCTGCAGCGCCGTGACCGGCGCAAAGTCCAGAAA
TCACTGGAGGATATTGCGGCAGGAAAGGTGCATACCACCTATCCCGTGGCGGAAATCAATCAGGGTGCCAAGGCTTCCTG
A

Upstream 100 bases:

>100_bases
GGTTATATATTACCCGCGGCTTTAGGTCTCGTGCGCTATCGGGCATCTCTCTGCGGGGCAACTTTTTGGCGGCTGCTCCG
GTTACTTAACAAAAACGATT

Downstream 100 bases:

>100_bases
GAGTTTTTTTCAGTGTTAATGGAGACAAAGAGAGGCTCATGCGAATTATTTTTATGGGAACTCCTGATTTTGCCGTTCCA
TCGTTGAAGGCTATTGCTGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MILPITIYSDEVLRRKAKPLKGIDTSHEELIGNMIESMRNASGIGLAAPQIGLSMRLLIVDLSPVQGYENAEPMVVINPH
ILAVKGYNAMEEGCLSIPDIHADVVRPSSIQLKYRNEHFEERVDEFSALMARVLQHEIDHLDGTLFVDKLQRRDRRKVQK
SLEDIAAGKVHTTYPVAEINQGAKAS

Sequences:

>Translated_186_residues
MILPITIYSDEVLRRKAKPLKGIDTSHEELIGNMIESMRNASGIGLAAPQIGLSMRLLIVDLSPVQGYENAEPMVVINPH
ILAVKGYNAMEEGCLSIPDIHADVVRPSSIQLKYRNEHFEERVDEFSALMARVLQHEIDHLDGTLFVDKLQRRDRRKVQK
SLEDIAAGKVHTTYPVAEINQGAKAS
>Mature_186_residues
MILPITIYSDEVLRRKAKPLKGIDTSHEELIGNMIESMRNASGIGLAAPQIGLSMRLLIVDLSPVQGYENAEPMVVINPH
ILAVKGYNAMEEGCLSIPDIHADVVRPSSIQLKYRNEHFEERVDEFSALMARVLQHEIDHLDGTLFVDKLQRRDRRKVQK
SLEDIAAGKVHTTYPVAEINQGAKAS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=31.055900621118, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=37.1951219512195, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24645726, Length=165, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PROA2 (B4S9B9)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016236.1
- ProteinModelPortal:   B4S9B9
- SMR:   B4S9B9
- GeneID:   6460438
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1571
- HOGENOM:   HBG665227
- OMA:   DHVENEL
- ProtClustDB:   PRK14598
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20778; Mature: 20778

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPITIYSDEVLRRKAKPLKGIDTSHEELIGNMIESMRNASGIGLAAPQIGLSMRLLIV
CEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DLSPVQGYENAEPMVVINPHILAVKGYNAMEEGCLSIPDIHADVVRPSSIQLKYRNEHFE
ECCCCCCCCCCCCEEEECCEEEEEECCCHHHHCCCCCCCCCHHHCCCCCEEEEECHHHHH
ERVDEFSALMARVLQHEIDHLDGTLFVDKLQRRDRRKVQKSLEDIAAGKVHTTYPVAEIN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
QGAKAS
CCCCCC
>Mature Secondary Structure
MILPITIYSDEVLRRKAKPLKGIDTSHEELIGNMIESMRNASGIGLAAPQIGLSMRLLIV
CEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DLSPVQGYENAEPMVVINPHILAVKGYNAMEEGCLSIPDIHADVVRPSSIQLKYRNEHFE
ECCCCCCCCCCCCEEEECCEEEEEECCCHHHHCCCCCCCCCHHHCCCCCEEEEECHHHHH
ERVDEFSALMARVLQHEIDHLDGTLFVDKLQRRDRRKVQKSLEDIAAGKVHTTYPVAEIN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
QGAKAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA