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The map label for this gene is fmt

Identifier: 194334375

GI number: 194334375

Start: 1709686

End: 1710639

Strand: Reverse

Name: fmt

Synonym: Paes_1570

Alternate gene names: 194334375

Gene position: 1710639-1709686 (Counterclockwise)

Preceding gene: 194334376

Following gene: 194334374

Centisome position: 68.07

GC content: 52.1

Gene sequence:

>954_bases
ATGCGAATTATTTTTATGGGAACTCCTGATTTTGCCGTTCCATCGTTGAAGGCTATTGCTGCTATGGACGGGGATTTCGA
GGTTCAGCTGGTTGTCACAGGGAAAGACAAGCCAAGGCGAAGCAAGCGATCTGAACCGGAACCCACGCCGGTTAAAAAAG
CAGCCAGAGAGCTCGGAATACCTGTTATGGAGGTCGATGATGTCAAGGATGAGCGTTTTGTCGATACAGTAGCCCGTTAC
CGGCCCGATGTTCTTGTCGTTGCCGCTTTCAGGATTCTTCCTCCCGCCGTCTATGAACAGGCCAGGCTGGGCGCATTCAA
TCTTCATGCATCCATTCTTCCTCGCTACCGGGGCGCAGCTCCGGTGAACTGGGCGATTATTAACGGCGAGCGGGAGAGTG
GTGTGACAACGTTTTTTCTTCGGAAATCGGTCGATACAGGCAACATGATTCTGCAGGAAAAAACCCCTGTCTATCCCGAA
GAAAATGCCGGAGAGCTGGCGGCAAGACTTGCAGAGATCGGGGCAGGCGCTGTTGTTAAGACGCTTGAGCTGATCCGCGA
TGGTCGCGTGGAGCCGTTATTGCAGAACGACACTCTGGCAACCAGAGCGCCGAAACTCTCGACGGAAAATACCAGGATCG
ACTGGGTACAGCCTGTCGACGCGCTCTGCGATTTTATTCGGGGCCTTGCGCCCCGTCCTGCTGCCTGGGCGATATTCAAC
CATAAGAAAGTTAAGATTTTCAAGGCATCTGTCGCTGATTTTCATCAGCCTGAAGCCGAAGAGCAGAGCGCTATTCCCGG
TAGGATCGCCGTTGAGAATAACAGGCTTTTTGTTATGGGGCTTGATGGATGGATCGAAATTCTCTCCTTGCAGATGGAGG
GTAAAAAAGTGATGCAGTCTCATGAGTTCTGCTGCGGGTTTCGCTGTGATGAGGAGTGTCCGGTATTTTCATAG

Upstream 100 bases:

>100_bases
CAGGAAAGGTGCATACCACCTATCCCGTGGCGGAAATCAATCAGGGTGCCAAGGCTTCCTGAGAGTTTTTTTCAGTGTTA
ATGGAGACAAAGAGAGGCTC

Downstream 100 bases:

>100_bases
ATCGATTGCTGCCCCTTTACTGGGAAATCGGCTGAATTCGTTATATTCGTTCCTTGTTTTTTCTGATAGAATTTCTACGT
AATTAAATACACGGTTTATG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGIPVMEVDDVKDERFVDTVARY
RPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAAPVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPE
ENAGELAARLAEIGAGAVVKTLELIRDGRVEPLLQNDTLATRAPKLSTENTRIDWVQPVDALCDFIRGLAPRPAAWAIFN
HKKVKIFKASVADFHQPEAEEQSAIPGRIAVENNRLFVMGLDGWIEILSLQMEGKKVMQSHEFCCGFRCDEECPVFS

Sequences:

>Translated_317_residues
MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGIPVMEVDDVKDERFVDTVARY
RPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAAPVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPE
ENAGELAARLAEIGAGAVVKTLELIRDGRVEPLLQNDTLATRAPKLSTENTRIDWVQPVDALCDFIRGLAPRPAAWAIFN
HKKVKIFKASVADFHQPEAEEQSAIPGRIAVENNRLFVMGLDGWIEILSLQMEGKKVMQSHEFCCGFRCDEECPVFS
>Mature_317_residues
MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGIPVMEVDDVKDERFVDTVARY
RPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAAPVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPE
ENAGELAARLAEIGAGAVVKTLELIRDGRVEPLLQNDTLATRAPKLSTENTRIDWVQPVDALCDFIRGLAPRPAAWAIFN
HKKVKIFKASVADFHQPEAEEQSAIPGRIAVENNRLFVMGLDGWIEILSLQMEGKKVMQSHEFCCGFRCDEECPVFS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=320, Percent_Identity=30.9375, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI238814322, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI21614513, Length=267, Percent_Identity=26.9662921348315, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789683, Length=311, Percent_Identity=38.5852090032154, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788589, Length=320, Percent_Identity=27.8125, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788846, Length=123, Percent_Identity=31.7073170731707, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133930964, Length=228, Percent_Identity=29.8245614035088, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550868, Length=316, Percent_Identity=27.8481012658228, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571984, Length=232, Percent_Identity=28.0172413793103, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585660, Length=320, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582400, Length=142, Percent_Identity=28.8732394366197, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PROA2 (B4S9B8)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016235.1
- ProteinModelPortal:   B4S9B8
- SMR:   B4S9B8
- GeneID:   6459724
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1570
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- ProtClustDB:   CLSK637772
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35272; Mature: 35272

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGI
CEEEEECCCCCCCCCHHHHEECCCCEEEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHCC
PVMEVDDVKDERFVDTVARYRPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAA
CEEEECCCCHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHCCEEEEHHHHHHHCCCCC
PVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPEENAGELAARLAEIGAGAVVK
CCEEEEECCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHH
TLELIRDGRVEPLLQNDTLATRAPKLSTENTRIDWVQPVDALCDFIRGLAPRPAAWAIFN
HHHHHHCCCCCCEECCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCEEEEEC
HKKVKIFKASVADFHQPEAEEQSAIPGRIAVENNRLFVMGLDGWIEILSLQMEGKKVMQS
CCEEEEEEECHHHCCCCCCHHHCCCCCEEEEECCEEEEEECCHHEEHHHHHCCCHHHHHH
HEFCCGFRCDEECPVFS
CCCEECCCCCCCCCCCC
>Mature Secondary Structure
MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGI
CEEEEECCCCCCCCCHHHHEECCCCEEEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHCC
PVMEVDDVKDERFVDTVARYRPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAA
CEEEECCCCHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHCCEEEEHHHHHHHCCCCC
PVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPEENAGELAARLAEIGAGAVVK
CCEEEEECCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHH
TLELIRDGRVEPLLQNDTLATRAPKLSTENTRIDWVQPVDALCDFIRGLAPRPAAWAIFN
HHHHHHCCCCCCEECCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCEEEEEC
HKKVKIFKASVADFHQPEAEEQSAIPGRIAVENNRLFVMGLDGWIEILSLQMEGKKVMQS
CCEEEEEEECHHHCCCCCCHHHCCCCCEEEEECCEEEEEECCHHEEHHHHHCCCHHHHHH
HEFCCGFRCDEECPVFS
CCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA