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The map label for this gene is rfbB [C]

Identifier: 194334317

GI number: 194334317

Start: 1651408

End: 1652433

Strand: Reverse

Name: rfbB [C]

Synonym: Paes_1510

Alternate gene names: 194334317

Gene position: 1652433-1651408 (Counterclockwise)

Preceding gene: 194334318

Following gene: 194334313

Centisome position: 65.76

GC content: 51.27

Gene sequence:

>1026_bases
TTGAATAAAATACTTGTTACCGGCGCAGCCGGTTTTATCGGTTTCCACGTCAGCCGCCGTCTGCTTGAACGCGGCGACGA
GGTCGTAGGCATTGATAACCTGAACAGCTATTACGATGTTGCGCTTAAAGAAGCGCGACTTGAAGAACTCGAGCCGTTCG
GGCAATTCCGGTTTATCCGTATGGACCTGGCCGACAGGGAGGCGATGGAGGACCTTTTTGCTATCGAAAAGTTTGACTAC
GTGGTCAATCTTGCGGCCCAGGCGGGAGTTCGCTACTCATTGCAGAATCCCCATGCTTATATCGACAGCAACATTCAGGG
GTTTATCAATATCCTTGAAGGGTGTCGTCATAACCATGTGGGGCATCTGGTCTATGCATCGTCGAGCTCTGTCTATGGTG
CGAACGAAACAATGCCTTTTTCGGTACATGACAATGTCGATCATCCGCTTTCACTCTACGCGGCGACCAAGAAGGCTAAC
GAACTGATGGCGCATACCTACAGCCATCTTTATCAGATTCCTACAACCGGTCTGCGTTTTTTCACCGTCTACGGTCCATG
GGGCAGACCCGATATGGCGCTTTTTCTCTTTACAAAGGCTATTGTGGAGGGAAAACCGATCAAGGTTTTCAATTACGGTA
AACACCGTCGAGATTTCACCTTCATCGACGATATTACGGAAGGGGTTATCAGAACGCTCGATCATGTCGCTGCCCCCAAT
CCTGAATGGTCCGGCCTCTCTCCCGATCCCGGTTCCAGCAGAGCACCCTGGAGAGTCTACAACATCGGTAACAGCAAGCC
GGTCAATCTGATGGATTATATCGACGCTCTTGAGCGCGAGCTTGGCAAAACCGCCGAGAAGGAGTTTCTGCCCATGCAGC
CTGGAGATGTTCCCGATACCTATGCAGATGTCGATCAGTTGATACAGGATGTAGACTATCAGCCCAAGACACCGGTAGCC
GAAGGGATCGGCAGGTTTGTGGAATGGTACAGAGGGTATTACGGCTGTTCGGTGCCAGGGGACTGA

Upstream 100 bases:

>100_bases
TTGTTGTGTATTTCGAAAGTATCATGCAGGGGATAGAGGTGACGATCAATGCGGAGTATCTCAGCCCTCTTTCCGATACG
TAAGTTCTAACAGGATTGCT

Downstream 100 bases:

>100_bases
TGTCTGAGAGATTGATGCGCTTCAGGTCCCCTACAGCGAATAATAGCTTGTCGTTTTATCGTTGCAAAGTTTTATCGTGC
CGGTTCTTACAAGGGTTACA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNKILVTGAAGFIGFHVSRRLLERGDEVVGIDNLNSYYDVALKEARLEELEPFGQFRFIRMDLADREAMEDLFAIEKFDY
VVNLAAQAGVRYSLQNPHAYIDSNIQGFINILEGCRHNHVGHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAATKKAN
ELMAHTYSHLYQIPTTGLRFFTVYGPWGRPDMALFLFTKAIVEGKPIKVFNYGKHRRDFTFIDDITEGVIRTLDHVAAPN
PEWSGLSPDPGSSRAPWRVYNIGNSKPVNLMDYIDALERELGKTAEKEFLPMQPGDVPDTYADVDQLIQDVDYQPKTPVA
EGIGRFVEWYRGYYGCSVPGD

Sequences:

>Translated_341_residues
MNKILVTGAAGFIGFHVSRRLLERGDEVVGIDNLNSYYDVALKEARLEELEPFGQFRFIRMDLADREAMEDLFAIEKFDY
VVNLAAQAGVRYSLQNPHAYIDSNIQGFINILEGCRHNHVGHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAATKKAN
ELMAHTYSHLYQIPTTGLRFFTVYGPWGRPDMALFLFTKAIVEGKPIKVFNYGKHRRDFTFIDDITEGVIRTLDHVAAPN
PEWSGLSPDPGSSRAPWRVYNIGNSKPVNLMDYIDALERELGKTAEKEFLPMQPGDVPDTYADVDQLIQDVDYQPKTPVA
EGIGRFVEWYRGYYGCSVPGD
>Mature_341_residues
MNKILVTGAAGFIGFHVSRRLLERGDEVVGIDNLNSYYDVALKEARLEELEPFGQFRFIRMDLADREAMEDLFAIEKFDY
VVNLAAQAGVRYSLQNPHAYIDSNIQGFINILEGCRHNHVGHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAATKKAN
ELMAHTYSHLYQIPTTGLRFFTVYGPWGRPDMALFLFTKAIVEGKPIKVFNYGKHRRDFTFIDDITEGVIRTLDHVAAPN
PEWSGLSPDPGSSRAPWRVYNIGNSKPVNLMDYIDALERELGKTAEKEFLPMQPGDVPDTYADVDQLIQDVDYQPKTPVA
EGIGRFVEWYRGYYGCSVPGD

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=26.4094955489614, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=23.7388724035608, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56237023, Length=323, Percent_Identity=26.9349845201238, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI56118217, Length=323, Percent_Identity=26.9349845201238, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI189083684, Length=323, Percent_Identity=26.9349845201238, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=24.7887323943662, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994969, Length=363, Percent_Identity=25.068870523416, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786974, Length=331, Percent_Identity=24.4712990936556, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788589, Length=351, Percent_Identity=24.7863247863248, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=24.5014245014245, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=350, Percent_Identity=24.2857142857143, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=24.4186046511628, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=24.5562130177515, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=28.0453257790368, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19923002, Length=322, Percent_Identity=27.0186335403727, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=22.8486646884273, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38488; Mature: 38488

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILVTGAAGFIGFHVSRRLLERGDEVVGIDNLNSYYDVALKEARLEELEPFGQFRFIR
CCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEE
MDLADREAMEDLFAIEKFDYVVNLAAQAGVRYSLQNPHAYIDSNIQGFINILEGCRHNHV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEECCCHHHHHHHHHHHCCCCC
GHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAATKKANELMAHTYSHLYQIPTTGLRF
EEEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
FTVYGPWGRPDMALFLFTKAIVEGKPIKVFNYGKHRRDFTFIDDITEGVIRTLDHVAAPN
EEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PEWSGLSPDPGSSRAPWRVYNIGNSKPVNLMDYIDALERELGKTAEKEFLPMQPGDVPDT
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCH
YADVDQLIQDVDYQPKTPVAEGIGRFVEWYRGYYGCSVPGD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNKILVTGAAGFIGFHVSRRLLERGDEVVGIDNLNSYYDVALKEARLEELEPFGQFRFIR
CCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEE
MDLADREAMEDLFAIEKFDYVVNLAAQAGVRYSLQNPHAYIDSNIQGFINILEGCRHNHV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEECCCHHHHHHHHHHHCCCCC
GHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAATKKANELMAHTYSHLYQIPTTGLRF
EEEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
FTVYGPWGRPDMALFLFTKAIVEGKPIKVFNYGKHRRDFTFIDDITEGVIRTLDHVAAPN
EEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PEWSGLSPDPGSSRAPWRVYNIGNSKPVNLMDYIDALERELGKTAEKEFLPMQPGDVPDT
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCH
YADVDQLIQDVDYQPKTPVAEGIGRFVEWYRGYYGCSVPGD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]