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The map label for this gene is mdtE [H]

Identifier: 194334208

GI number: 194334208

Start: 1521893

End: 1523092

Strand: Reverse

Name: mdtE [H]

Synonym: Paes_1400

Alternate gene names: 194334208

Gene position: 1523092-1521893 (Counterclockwise)

Preceding gene: 194334209

Following gene: 194334207

Centisome position: 60.61

GC content: 53.67

Gene sequence:

>1200_bases
ATGACCAATATCAGAAAACGATTGAGTTCGTTTTGCCTTGGCAGTACGATACTGCTTCTCTCGCTTTCAGGGTGTGAGGG
GCAAAATGCCAGGCAGGCTGCCAATCAGCAGCAGCCGCCTTCCCTTCCTGTCATGAACGTGATGCGTTCTTCCGTCAGCG
TCAATCAGGAGTATGCCGCATTGATTGAGGGTGTTACTACTGTCGAAATAAGACCTCAGGTTGAGGGTGTTTTGAAGGAG
ATCGCTGTTGACGAAGGGGCGTATGTCAACAAGGGAGATCTGCTGTTCACTATTGATGACCGGATATATCGCCAGGAGCT
GCAGACTGCTGTGGCGGCAAAGAAGTCGGCAGAGGCCGCTCTTGCAATCGCTGCTCTTGATGTTGAAAAGCTCAAGCCTC
TGGTGCGCAACAACGTGGTTTCAGAGGTGCAGCTTCAAGAGGCCCGGGCTCATCTGCGTGCAGCGAAGGCCGCTCTCGAT
CAGGCTGCAGCAAAGGAGCAAATAGCCCGGATCAATCTCGAGTATAGCAGCATAACGGCACCAGTCAACGGTTATATCGG
TGCGATAGCGTTTCGGACGGGCAGTCTTGTGAGCCGCAACCAGTCGGATAAGCTGACGACGCTCTCCGATGTCCATCAGG
TCAGAGCGTATTTCAGCATGAGCGAAGTTGATTTTGTCCGTTTTCGGCAGCGCTATCCAGGCGGGTCAATTGAAGAGCCG
CTTTCAGCCGTGCCTCCGGTGCATCTCCTGCTGGCTGACGGCACTACTTTTGGCAACGAGGGCCGGCTTGATGCGATCAA
CGGGCAGTTCGACCGCACGACAGCATCTGTTACCCTTCGGGCGACTTTTCCGAATCCCGATGGGCTTCTCAGGTCGGGCA
ATACGGGTAAAGTTGTGATTGGTTATACGTATGACGATATCATTCTTGTTCCGCAGTCGGCAACGTCGGATCTGCAGGAC
AGGATTTTTGTGAGCAAGGTCAACGAGGGCAATGCTGTGGCGCGTACCCCGGTGACGGTTATCGGACGGAGCGGAAATGA
TTATATCGTCAGTGGAGGTCTTGAGGATGGTGATATGATTATTCTCTCAGGCTTCAGCCGTCTGCCTGACGGAACGGTCA
TCAGTCCGCTGCAGAAGGCCGGAGAGGAGAGTGCGCTTCCTGAAGAAGAACTGAAGATCAACGAGAAAGAGGGCGTGTAA

Upstream 100 bases:

>100_bases
ATGCTATAACACATGACGGGCTGGATGTTTGCGCTCTGCGAGCGGCCTGCTGTTCAGGTGGCGTCATTCCGGAAACCGGT
CGAAATACAGTTACAGAACT

Downstream 100 bases:

>100_bases
ACTATGTTTGATCGATTTATTAACAGACCGGTTCTGGCAACGGTTATATCGGTATTTTTTGTCCTACTCGGTCTGATCGG
TATCAGCCAGCTGCCGCTGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTNIRKRLSSFCLGSTILLLSLSGCEGQNARQAANQQQPPSLPVMNVMRSSVSVNQEYAALIEGVTTVEIRPQVEGVLKE
IAVDEGAYVNKGDLLFTIDDRIYRQELQTAVAAKKSAEAALAIAALDVEKLKPLVRNNVVSEVQLQEARAHLRAAKAALD
QAAAKEQIARINLEYSSITAPVNGYIGAIAFRTGSLVSRNQSDKLTTLSDVHQVRAYFSMSEVDFVRFRQRYPGGSIEEP
LSAVPPVHLLLADGTTFGNEGRLDAINGQFDRTTASVTLRATFPNPDGLLRSGNTGKVVIGYTYDDIILVPQSATSDLQD
RIFVSKVNEGNAVARTPVTVIGRSGNDYIVSGGLEDGDMIILSGFSRLPDGTVISPLQKAGEESALPEEELKINEKEGV

Sequences:

>Translated_399_residues
MTNIRKRLSSFCLGSTILLLSLSGCEGQNARQAANQQQPPSLPVMNVMRSSVSVNQEYAALIEGVTTVEIRPQVEGVLKE
IAVDEGAYVNKGDLLFTIDDRIYRQELQTAVAAKKSAEAALAIAALDVEKLKPLVRNNVVSEVQLQEARAHLRAAKAALD
QAAAKEQIARINLEYSSITAPVNGYIGAIAFRTGSLVSRNQSDKLTTLSDVHQVRAYFSMSEVDFVRFRQRYPGGSIEEP
LSAVPPVHLLLADGTTFGNEGRLDAINGQFDRTTASVTLRATFPNPDGLLRSGNTGKVVIGYTYDDIILVPQSATSDLQD
RIFVSKVNEGNAVARTPVTVIGRSGNDYIVSGGLEDGDMIILSGFSRLPDGTVISPLQKAGEESALPEEELKINEKEGV
>Mature_398_residues
TNIRKRLSSFCLGSTILLLSLSGCEGQNARQAANQQQPPSLPVMNVMRSSVSVNQEYAALIEGVTTVEIRPQVEGVLKEI
AVDEGAYVNKGDLLFTIDDRIYRQELQTAVAAKKSAEAALAIAALDVEKLKPLVRNNVVSEVQLQEARAHLRAAKAALDQ
AAAKEQIARINLEYSSITAPVNGYIGAIAFRTGSLVSRNQSDKLTTLSDVHQVRAYFSMSEVDFVRFRQRYPGGSIEEPL
SAVPPVHLLLADGTTFGNEGRLDAINGQFDRTTASVTLRATFPNPDGLLRSGNTGKVVIGYTYDDIILVPQSATSDLQDR
IFVSKVNEGNAVARTPVTVIGRSGNDYIVSGGLEDGDMIILSGFSRLPDGTVISPLQKAGEESALPEEELKINEKEGV

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=359, Percent_Identity=30.6406685236769, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789665, Length=368, Percent_Identity=31.25, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786668, Length=354, Percent_Identity=29.3785310734463, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082036, Length=332, Percent_Identity=25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081796, Length=320, Percent_Identity=25.9375, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081951, Length=162, Percent_Identity=30.2469135802469, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43031; Mature: 42900

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIRKRLSSFCLGSTILLLSLSGCEGQNARQAANQQQPPSLPVMNVMRSSVSVNQEYAA
CCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHH
LIEGVTTVEIRPQVEGVLKEIAVDEGAYVNKGDLLFTIDDRIYRQELQTAVAAKKSAEAA
HHCCCEEEEECHHHHHHHHHHHCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHCCHHE
LAIAALDVEKLKPLVRNNVVSEVQLQEARAHLRAAKAALDQAAAKEQIARINLEYSSITA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHEEC
PVNGYIGAIAFRTGSLVSRNQSDKLTTLSDVHQVRAYFSMSEVDFVRFRQRYPGGSIEEP
CCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LSAVPPVHLLLADGTTFGNEGRLDAINGQFDRTTASVTLRATFPNPDGLLRSGNTGKVVI
HHHCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCEECCCCCEEEE
GYTYDDIILVPQSATSDLQDRIFVSKVNEGNAVARTPVTVIGRSGNDYIVSGGLEDGDMI
EEEECCEEEECCCCCCHHHHCEEEEECCCCCEEEECCEEEEECCCCCEEEECCCCCCCEE
ILSGFSRLPDGTVISPLQKAGEESALPEEELKINEKEGV
EEECCCCCCCCCEEHHHHHCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
TNIRKRLSSFCLGSTILLLSLSGCEGQNARQAANQQQPPSLPVMNVMRSSVSVNQEYAA
CHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHH
LIEGVTTVEIRPQVEGVLKEIAVDEGAYVNKGDLLFTIDDRIYRQELQTAVAAKKSAEAA
HHCCCEEEEECHHHHHHHHHHHCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHCCHHE
LAIAALDVEKLKPLVRNNVVSEVQLQEARAHLRAAKAALDQAAAKEQIARINLEYSSITA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHEEC
PVNGYIGAIAFRTGSLVSRNQSDKLTTLSDVHQVRAYFSMSEVDFVRFRQRYPGGSIEEP
CCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LSAVPPVHLLLADGTTFGNEGRLDAINGQFDRTTASVTLRATFPNPDGLLRSGNTGKVVI
HHHCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCEECCCCCEEEE
GYTYDDIILVPQSATSDLQDRIFVSKVNEGNAVARTPVTVIGRSGNDYIVSGGLEDGDMI
EEEECCEEEECCCCCCHHHHCEEEEECCCCCEEEECCEEEEECCCCCEEEECCCCCCCEE
ILSGFSRLPDGTVISPLQKAGEESALPEEELKINEKEGV
EEECCCCCCCCCEEHHHHHCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]