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The map label for this gene is cbiF [H]

Identifier: 194334100

GI number: 194334100

Start: 1405703

End: 1407583

Strand: Reverse

Name: cbiF [H]

Synonym: Paes_1288

Alternate gene names: 194334100

Gene position: 1407583-1405703 (Counterclockwise)

Preceding gene: 194334101

Following gene: 194334099

Centisome position: 56.01

GC content: 59.76

Gene sequence:

>1881_bases
ATGTCATCAGACCGCCTCGCTATTATCGCCGCTTCTCAGCAGGGAATCATGACCGCTTTACGCCTGAAACAGGAACTCGC
AGCGTGCGGAACCACTGAGGTCGCCCTGTTTTCACCGAGGGCCGGGGCCGAATCGACCAGAATCAGCTCGATCACGGAGT
GGACTGCAGAGGAGTTTCACAACTGGGATGCACTGGTGTACATCGGTGCCCTCGGCATCTGCGTCCGCGCGGTCGCCCCG
GTGCTTCAGAGCAAGAAGAGCGATCCGGCCGTCATCAACTGTGATGAACAGGGGCGGTTCGTGCAGTCCGTCCTGTCAGG
GCATTACGGGGGCGCCAACGATCTTGCCCGCAGGGTTTCACGCATGCTTGGAGCGCAGCCGGTCATTACCACCTCAAGCG
ACGTGCAGGGCCTGTGGCCTCTCGATATTCTCGGGCGTGACGAGGGATGGGGGGCAGAGTACCGTGCCGGGCTCGAGGGG
AGGTCGCTGACGGATGCACAGGCCGCCTTTGTCAACCATGAACCGACGGTTCTGCTGCTCGATGTCCGCGATGAACTGAC
CGATCGGCTCGAGAGAACCTGCCCGGACTTCGTAACTGTCGCTTATCGCTACGAGGATGTCGATGTCGAGTCCTGTTCGC
TGCTGCTTGCGGTGACCCCCTTCCTGTATGAGCCTCCTGTCCAGGCCGTGTTCTACCGGCCCCGTGTCCTGTGCGTGGGA
CTGGGTTCAGAGAAAGGTATCGATCCGGAGCGTTTTGTCGGCTCGTTTCTTCACCGGCTCCGGGAAAAGCGCCTCTCGTA
CCAATCGGTTACAGCTCTTGCTACAGTGGATTTCAAGGTGCAGGAACCTGCATTCCAGACGCTGACGAGAGAACTCGGAA
TCGGTCTGCACGGCTATGACGCTCAGGCGCTCGAAGCAGTCGGTGGCGTGCCGAACCCATCCGAAACCGTGTTTCAAAAA
GTCGGAATACACAGTGTTTCCGAGGCTGCTTCAGCCCTGCTCGCCGGTCATGAGGAGTGGATTGTCGAAAAACAGAAAGT
TGCACTCGATTATGTCGAAAAGGGTCAGCCGCGCCATTTTACGTTTGCCGTCTCCATGAAGCAGGATGCCCTGCGGCGTG
GCCATATTTCCATTGTCGGGGCCGGTCCGGGTGACCCGGGCCTGGTGACGGTCAGGGGCAGGGAGCTCATCGAGGCCGCT
GACCTTGTACTGTATGCAGGGAGCCTCGTGCCGGAAAAGCTCACGGAGTATGCTCATGCCGGGGCGCTGGTGCGCAGTTC
TGCGTCGATGTCGCTTGAGGAGCAGTTCGAGCTGATGAAACGGTTCTGCCTCCAGGGAAAACAGGTCGTACGCCTGCATA
CGGGAGACCCCTGTATTTACGGAGCGATCCAGGAGCAGATAGCATGGTTCGAGGCGCATGAGATGCCCTACGATATCGTG
CCGGGCGTATCGTCCTTCCAGGCGGCGGCGGCCGCATTGAACTCCCAGTTTACGGTGCCAGAAAAAGTGCAGACTATTAT
TCTCACCAGGGGGAACGGGCGGACGCCGGTCCCGGAAAAAGAACGGTTGCGCGACCTCGCTCGCGCACGGGCAACCATGT
GTATTTTCCTTAGCGCAGAATGGGCCGACCAGGTGCAGCGGGAGCTCGAGGCGGAGTACCCGCCGACGACGCCGGTTGCT
GTGTGTTACCGCCTGAGCTGGGACGACCAACAGGTATGGAGAGGAGAGCTTGGCAGCCTTGCGGCAATGGTTCGCGAAAG
CGGAAAAACCCGGACGGTTCTGCTCGTCGTAGGTGAAGCCATCGGGGCGCGACAAAACCGTTCGAAACTCTACGATCCGC
ATTTCACTCATGGGTTCAGGCGCTCGGCCAGGGAGGAATGA

Upstream 100 bases:

>100_bases
ACCATGCGCTTCGCCTGCCCGATGACCTTGTGATCACTCCCGGGAGGCATAATTCAATAACCATCGCTGCCGCAGTGCAG
GAATAACTACATGTGTTTCT

Downstream 100 bases:

>100_bases
GAGATGATTATCGTGTTTGGCGGAACGACCGAGGGGAAAAAGGTGGCCGGATTTCTCGACAGGAAATCCATGCCGTACCT
GTATTCGACCAAAATGGAGT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MSSDRLAIIAASQQGIMTALRLKQELAACGTTEVALFSPRAGAESTRISSITEWTAEEFHNWDALVYIGALGICVRAVAP
VLQSKKSDPAVINCDEQGRFVQSVLSGHYGGANDLARRVSRMLGAQPVITTSSDVQGLWPLDILGRDEGWGAEYRAGLEG
RSLTDAQAAFVNHEPTVLLLDVRDELTDRLERTCPDFVTVAYRYEDVDVESCSLLLAVTPFLYEPPVQAVFYRPRVLCVG
LGSEKGIDPERFVGSFLHRLREKRLSYQSVTALATVDFKVQEPAFQTLTRELGIGLHGYDAQALEAVGGVPNPSETVFQK
VGIHSVSEAASALLAGHEEWIVEKQKVALDYVEKGQPRHFTFAVSMKQDALRRGHISIVGAGPGDPGLVTVRGRELIEAA
DLVLYAGSLVPEKLTEYAHAGALVRSSASMSLEEQFELMKRFCLQGKQVVRLHTGDPCIYGAIQEQIAWFEAHEMPYDIV
PGVSSFQAAAAALNSQFTVPEKVQTIILTRGNGRTPVPEKERLRDLARARATMCIFLSAEWADQVQRELEAEYPPTTPVA
VCYRLSWDDQQVWRGELGSLAAMVRESGKTRTVLLVVGEAIGARQNRSKLYDPHFTHGFRRSAREE

Sequences:

>Translated_626_residues
MSSDRLAIIAASQQGIMTALRLKQELAACGTTEVALFSPRAGAESTRISSITEWTAEEFHNWDALVYIGALGICVRAVAP
VLQSKKSDPAVINCDEQGRFVQSVLSGHYGGANDLARRVSRMLGAQPVITTSSDVQGLWPLDILGRDEGWGAEYRAGLEG
RSLTDAQAAFVNHEPTVLLLDVRDELTDRLERTCPDFVTVAYRYEDVDVESCSLLLAVTPFLYEPPVQAVFYRPRVLCVG
LGSEKGIDPERFVGSFLHRLREKRLSYQSVTALATVDFKVQEPAFQTLTRELGIGLHGYDAQALEAVGGVPNPSETVFQK
VGIHSVSEAASALLAGHEEWIVEKQKVALDYVEKGQPRHFTFAVSMKQDALRRGHISIVGAGPGDPGLVTVRGRELIEAA
DLVLYAGSLVPEKLTEYAHAGALVRSSASMSLEEQFELMKRFCLQGKQVVRLHTGDPCIYGAIQEQIAWFEAHEMPYDIV
PGVSSFQAAAAALNSQFTVPEKVQTIILTRGNGRTPVPEKERLRDLARARATMCIFLSAEWADQVQRELEAEYPPTTPVA
VCYRLSWDDQQVWRGELGSLAAMVRESGKTRTVLLVVGEAIGARQNRSKLYDPHFTHGFRRSAREE
>Mature_625_residues
SSDRLAIIAASQQGIMTALRLKQELAACGTTEVALFSPRAGAESTRISSITEWTAEEFHNWDALVYIGALGICVRAVAPV
LQSKKSDPAVINCDEQGRFVQSVLSGHYGGANDLARRVSRMLGAQPVITTSSDVQGLWPLDILGRDEGWGAEYRAGLEGR
SLTDAQAAFVNHEPTVLLLDVRDELTDRLERTCPDFVTVAYRYEDVDVESCSLLLAVTPFLYEPPVQAVFYRPRVLCVGL
GSEKGIDPERFVGSFLHRLREKRLSYQSVTALATVDFKVQEPAFQTLTRELGIGLHGYDAQALEAVGGVPNPSETVFQKV
GIHSVSEAASALLAGHEEWIVEKQKVALDYVEKGQPRHFTFAVSMKQDALRRGHISIVGAGPGDPGLVTVRGRELIEAAD
LVLYAGSLVPEKLTEYAHAGALVRSSASMSLEEQFELMKRFCLQGKQVVRLHTGDPCIYGAIQEQIAWFEAHEMPYDIVP
GVSSFQAAAAALNSQFTVPEKVQTIILTRGNGRTPVPEKERLRDLARARATMCIFLSAEWADQVQRELEAEYPPTTPVAV
CYRLSWDDQQVWRGELGSLAAMVRESGKTRTVLLVVGEAIGARQNRSKLYDPHFTHGFRRSAREE

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=28.1632653061224, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322922, Length=270, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 68884; Mature: 68753

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDRLAIIAASQQGIMTALRLKQELAACGTTEVALFSPRAGAESTRISSITEWTAEEFH
CCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
NWDALVYIGALGICVRAVAPVLQSKKSDPAVINCDEQGRFVQSVLSGHYGGANDLARRVS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHH
RMLGAQPVITTSSDVQGLWPLDILGRDEGWGAEYRAGLEGRSLTDAQAAFVNHEPTVLLL
HHHCCCCEEECCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCEEEE
DVRDELTDRLERTCPDFVTVAYRYEDVDVESCSLLLAVTPFLYEPPVQAVFYRPRVLCVG
ECHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHEECCHHHCCCCHHHHEECCEEEEEE
LGSEKGIDPERFVGSFLHRLREKRLSYQSVTALATVDFKVQEPAFQTLTRELGIGLHGYD
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEEECCHHHHHHHHHHCCCCCCCC
AQALEAVGGVPNPSETVFQKVGIHSVSEAASALLAGHEEWIVEKQKVALDYVEKGQPRHF
HHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
TFAVSMKQDALRRGHISIVGAGPGDPGLVTVRGRELIEAADLVLYAGSLVPEKLTEYAHA
EEEEECCHHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
GALVRSSASMSLEEQFELMKRFCLQGKQVVRLHTGDPCIYGAIQEQIAWFEAHEMPYDIV
HHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCHHC
PGVSSFQAAAAALNSQFTVPEKVQTIILTRGNGRTPVPEKERLRDLARARATMCIFLSAE
CCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECH
WADQVQRELEAEYPPTTPVAVCYRLSWDDQQVWRGELGSLAAMVRESGKTRTVLLVVGEA
HHHHHHHHHCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
IGARQNRSKLYDPHFTHGFRRSAREE
HCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SSDRLAIIAASQQGIMTALRLKQELAACGTTEVALFSPRAGAESTRISSITEWTAEEFH
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
NWDALVYIGALGICVRAVAPVLQSKKSDPAVINCDEQGRFVQSVLSGHYGGANDLARRVS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHH
RMLGAQPVITTSSDVQGLWPLDILGRDEGWGAEYRAGLEGRSLTDAQAAFVNHEPTVLLL
HHHCCCCEEECCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHCCCCCCEEEE
DVRDELTDRLERTCPDFVTVAYRYEDVDVESCSLLLAVTPFLYEPPVQAVFYRPRVLCVG
ECHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHEECCHHHCCCCHHHHEECCEEEEEE
LGSEKGIDPERFVGSFLHRLREKRLSYQSVTALATVDFKVQEPAFQTLTRELGIGLHGYD
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEEECCHHHHHHHHHHCCCCCCCC
AQALEAVGGVPNPSETVFQKVGIHSVSEAASALLAGHEEWIVEKQKVALDYVEKGQPRHF
HHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
TFAVSMKQDALRRGHISIVGAGPGDPGLVTVRGRELIEAADLVLYAGSLVPEKLTEYAHA
EEEEECCHHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
GALVRSSASMSLEEQFELMKRFCLQGKQVVRLHTGDPCIYGAIQEQIAWFEAHEMPYDIV
HHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCHHC
PGVSSFQAAAAALNSQFTVPEKVQTIILTRGNGRTPVPEKERLRDLARARATMCIFLSAE
CCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECH
WADQVQRELEAEYPPTTPVAVCYRLSWDDQQVWRGELGSLAAMVRESGKTRTVLLVVGEA
HHHHHHHHHCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCH
IGARQNRSKLYDPHFTHGFRRSAREE
HCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]