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The map label for this gene is cobQ

Identifier: 194334082

GI number: 194334082

Start: 1372718

End: 1374199

Strand: Reverse

Name: cobQ

Synonym: Paes_1269

Alternate gene names: 194334082

Gene position: 1374199-1372718 (Counterclockwise)

Preceding gene: 194334083

Following gene: 194334081

Centisome position: 54.69

GC content: 51.89

Gene sequence:

>1482_bases
ATGAAGAATCTCGCGGTTTTCGGCACTGCATCCGATGTCGGCAAAAGCGTCATTGCGACCGCCTTGTGCAGAATATTCAG
CAACGAGGGTATTGATGTGGCACCCTTCAAGGCGCAGAATATGTCCAACAACTCCGGGGTGACACCTGATGGTTTTGAGA
TAGGTCGGGCACAGGTGGTGCAGGCAGAAGCGGCCAGGGTGGTCCCGACTGCGGACATGAACCCTGTTCTGCTCAAACCC
AATACCGATACCGGCGCTCAGGTTGTACTGCAGGGCAAAGCGGTTGCGAACCGTTCAGCCAGGGATTATTTCGGCAACAC
CCGGCCCTGGGCTGAAGCTGCGTTCGAGAGTCTCAGGCGGCTTCAGGCAGCCCACGATATGCTTGTGATCGAGGGGGCTG
GATCATGTGCGGAAATGAATCTTTACGACAGGGACTTTGTCAATTTTAAAACTGCCGAAGCAGCTGATGCTCCGGTGATT
CTTGTCGCCGATATCGATCGGGGAGGTGTGTTCGCTCAGGTGATCGGAACAGTTTCGGTGCTGCCTCCGGAAGCAAAAGC
GCGGATTTCAGGCGTGCTGGTCAACCGTTTTCGTGGAGACATCTCGCTGTTTGAGGATGGTGTTCGTTTTCTTGAAGAGC
ATTCAGGGATTCCTGTGCTCGGTGTTGTTCCTTTTTTCCGGGGATTTTCGATTGATTCCGAGGACGCCGTTCCGCTACAG
ACCATTGTCGATCCCTCAGGCTCGCCTGACCCCGGGAAAATCAGTATTGCTGTCATCTATTTTCCTCATATTTCAAATTT
TACCGATTTTGCTGTTTTGGAACGCGAGGAGGGTGTTGAGCTGCATTATCTGCATCATGCAAAAGCCCTTGATGGCTATC
AGGCGGTTATCCTGCCGGGGTCGAAAAACGTTCGCGGTGATATTGAGTGGTTACGCCAGCAGGGGTGGGAGAGCCGTCTT
GAGGATTTTCGTCGGAATGGTGGTGTTATTGCCGGTATTTGCGGCGGTTATCAGATGCTTGGGCGTTCTATTGCTGACCC
GTATGGCGTCGAAGGGGTTCCAGGCAGGACAGCAGGCTTGGATCTGCTGCCAGTCGAAACTGTTCTTCAGCAGGAAAAGA
CACTGTGCAACGCCAGGGGCGTTATGAGCGGTAACGGTTATAGCGTGCATGGTTATGAAATTCATATGGGTGAAACCTCC
TGCCATGGCGATGCGCTTCCTCTTTTTGACGTGACGACACGAAACGGTATTGCTGTTTCTGCTCGCGACGGTGTCATGAC
TTCTGATACAAAGGTCTTCGGGACCTATTTTCATGGTGTTTTTGATGCCCCGCTTTTTCGGCACTGGTTTTTCAGACAAC
TGAGTGCATCCTATACTCCGTCTGAAAGAACAGAGGACAGGGATGAGGATTATAACCGGCTTGCGCGACATGTTGCAGCT
CATCTTGATCTTGATAAGCTGTACAGGATTATCGAAAAATAG

Upstream 100 bases:

>100_bases
CTTTTTTTCATGAAACGCCTGAGCTTGAACAGGCCGGTTGGCTTCCGAGAATGGAGCTTGACAGACTTGTTCACCGTGAA
CAGTGGGGACAGAGAGCGCT

Downstream 100 bases:

>100_bases
GAGTAAGTTGTTTTTTTCGAATACCGAACAGGGGACAATGAGCAGAGACAATCTTTATCATCATGAACACGGAGGAGAAC
CGGAGCGCTACTGCGTTACA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKNLAVFGTASDVGKSVIATALCRIFSNEGIDVAPFKAQNMSNNSGVTPDGFEIGRAQVVQAEAARVVPTADMNPVLLKP
NTDTGAQVVLQGKAVANRSARDYFGNTRPWAEAAFESLRRLQAAHDMLVIEGAGSCAEMNLYDRDFVNFKTAEAADAPVI
LVADIDRGGVFAQVIGTVSVLPPEAKARISGVLVNRFRGDISLFEDGVRFLEEHSGIPVLGVVPFFRGFSIDSEDAVPLQ
TIVDPSGSPDPGKISIAVIYFPHISNFTDFAVLEREEGVELHYLHHAKALDGYQAVILPGSKNVRGDIEWLRQQGWESRL
EDFRRNGGVIAGICGGYQMLGRSIADPYGVEGVPGRTAGLDLLPVETVLQQEKTLCNARGVMSGNGYSVHGYEIHMGETS
CHGDALPLFDVTTRNGIAVSARDGVMTSDTKVFGTYFHGVFDAPLFRHWFFRQLSASYTPSERTEDRDEDYNRLARHVAA
HLDLDKLYRIIEK

Sequences:

>Translated_493_residues
MKNLAVFGTASDVGKSVIATALCRIFSNEGIDVAPFKAQNMSNNSGVTPDGFEIGRAQVVQAEAARVVPTADMNPVLLKP
NTDTGAQVVLQGKAVANRSARDYFGNTRPWAEAAFESLRRLQAAHDMLVIEGAGSCAEMNLYDRDFVNFKTAEAADAPVI
LVADIDRGGVFAQVIGTVSVLPPEAKARISGVLVNRFRGDISLFEDGVRFLEEHSGIPVLGVVPFFRGFSIDSEDAVPLQ
TIVDPSGSPDPGKISIAVIYFPHISNFTDFAVLEREEGVELHYLHHAKALDGYQAVILPGSKNVRGDIEWLRQQGWESRL
EDFRRNGGVIAGICGGYQMLGRSIADPYGVEGVPGRTAGLDLLPVETVLQQEKTLCNARGVMSGNGYSVHGYEIHMGETS
CHGDALPLFDVTTRNGIAVSARDGVMTSDTKVFGTYFHGVFDAPLFRHWFFRQLSASYTPSERTEDRDEDYNRLARHVAA
HLDLDKLYRIIEK
>Mature_493_residues
MKNLAVFGTASDVGKSVIATALCRIFSNEGIDVAPFKAQNMSNNSGVTPDGFEIGRAQVVQAEAARVVPTADMNPVLLKP
NTDTGAQVVLQGKAVANRSARDYFGNTRPWAEAAFESLRRLQAAHDMLVIEGAGSCAEMNLYDRDFVNFKTAEAADAPVI
LVADIDRGGVFAQVIGTVSVLPPEAKARISGVLVNRFRGDISLFEDGVRFLEEHSGIPVLGVVPFFRGFSIDSEDAVPLQ
TIVDPSGSPDPGKISIAVIYFPHISNFTDFAVLEREEGVELHYLHHAKALDGYQAVILPGSKNVRGDIEWLRQQGWESRL
EDFRRNGGVIAGICGGYQMLGRSIADPYGVEGVPGRTAGLDLLPVETVLQQEKTLCNARGVMSGNGYSVHGYEIHMGETS
CHGDALPLFDVTTRNGIAVSARDGVMTSDTKVFGTYFHGVFDAPLFRHWFFRQLSASYTPSERTEDRDEDYNRLARHVAA
HLDLDKLYRIIEK

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_PROA2 (B4S8A8)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015942.1
- ProteinModelPortal:   B4S8A8
- GeneID:   6460498
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1269
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 53751; Mature: 53751

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLAVFGTASDVGKSVIATALCRIFSNEGIDVAPFKAQNMSNNSGVTPDGFEIGRAQVV
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHEE
QAEAARVVPTADMNPVLLKPNTDTGAQVVLQGKAVANRSARDYFGNTRPWAEAAFESLRR
EHHHHEECCCCCCCCEEECCCCCCCCEEEEECCHHHCCCHHHHCCCCCCHHHHHHHHHHH
LQAAHDMLVIEGAGSCAEMNLYDRDFVNFKTAEAADAPVILVADIDRGGVFAQVIGTVSV
HHHHCCEEEEECCCCEEECCCCCCCCCCEECCCCCCCCEEEEEECCCCCCHHHHHHHHCC
LPPEAKARISGVLVNRFRGDISLFEDGVRFLEEHSGIPVLGVVPFFRGFSIDSEDAVPLQ
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCCCHH
TIVDPSGSPDPGKISIAVIYFPHISNFTDFAVLEREEGVELHYLHHAKALDGYQAVILPG
EEECCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEHHHHCCCCEEEEECC
SKNVRGDIEWLRQQGWESRLEDFRRNGGVIAGICGGYQMLGRSIADPYGVEGVPGRTAGL
CCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCC
DLLPVETVLQQEKTLCNARGVMSGNGYSVHGYEIHMGETSCHGDALPLFDVTTRNGIAVS
EEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCEEEE
ARDGVMTSDTKVFGTYFHGVFDAPLFRHWFFRQLSASYTPSERTEDRDEDYNRLARHVAA
ECCCCEECCCEEHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
HLDLDKLYRIIEK
HCCHHHHHHHHCC
>Mature Secondary Structure
MKNLAVFGTASDVGKSVIATALCRIFSNEGIDVAPFKAQNMSNNSGVTPDGFEIGRAQVV
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHEE
QAEAARVVPTADMNPVLLKPNTDTGAQVVLQGKAVANRSARDYFGNTRPWAEAAFESLRR
EHHHHEECCCCCCCCEEECCCCCCCCEEEEECCHHHCCCHHHHCCCCCCHHHHHHHHHHH
LQAAHDMLVIEGAGSCAEMNLYDRDFVNFKTAEAADAPVILVADIDRGGVFAQVIGTVSV
HHHHCCEEEEECCCCEEECCCCCCCCCCEECCCCCCCCEEEEEECCCCCCHHHHHHHHCC
LPPEAKARISGVLVNRFRGDISLFEDGVRFLEEHSGIPVLGVVPFFRGFSIDSEDAVPLQ
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCCCHH
TIVDPSGSPDPGKISIAVIYFPHISNFTDFAVLEREEGVELHYLHHAKALDGYQAVILPG
EEECCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEHHHHCCCCEEEEECC
SKNVRGDIEWLRQQGWESRLEDFRRNGGVIAGICGGYQMLGRSIADPYGVEGVPGRTAGL
CCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCC
DLLPVETVLQQEKTLCNARGVMSGNGYSVHGYEIHMGETSCHGDALPLFDVTTRNGIAVS
EEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCEEEE
ARDGVMTSDTKVFGTYFHGVFDAPLFRHWFFRQLSASYTPSERTEDRDEDYNRLARHVAA
ECCCCEECCCEEHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
HLDLDKLYRIIEK
HCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA