Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 194334065

GI number: 194334065

Start: 1354898

End: 1355644

Strand: Reverse

Name: cobS

Synonym: Paes_1252

Alternate gene names: 194334065

Gene position: 1355644-1354898 (Counterclockwise)

Preceding gene: 194334066

Following gene: 194334064

Centisome position: 53.95

GC content: 53.15

Gene sequence:

>747_bases
ATGCTCGACGGTCTTGTCACTGCTATGCGGACTCTCACTGTTCTTTCTGTGCCCGGAAAAGATGCAGACGAGTTCAGTCG
TTCCCTCTACTGGTTTCCTCTGGTTGGTCTTTTGCTTGGTCTGCTGCAGGCTGCATTGGCATGGATCGGGATGGTGTCGC
AAATACCGGAATTTTCTGCGCTGCTCGTGCTCCTCTCGGGGGTTCTTCTGACCAGAGCGATTCACGCTGACGGCCTTGCT
GATCTTGCAGATGGTTTTTTCGGCGGAAAAACCAGGGAGTCCCGGTTGCGTATTATGAAAGACCCGGCAGTCGGATCATT
TGGCGTTATCGCCCTGATTCTGCTTTTTCTTTTTAAGTGGATTGCGCTTACCCGTATTGTGGCTCATGGGCAGTATGAGT
GGATTGTCAGCGGTATCGTGCTCGCTCGTTTTGTGCAGGTCGTTCTTGCATCCGTCATGACATATGCGCGAGAGGGGGAG
GGAACGGCTTGCCGATTCGTTGCCGGTGCCGGTGGCTGGCATGTGGTTGTTGCTGCTCTGTTTTCTCTGCTCATTCTTGT
GCTGGTCATGAAGATGCAACGCCTGCCAATCGTCGTCGCTCTTCTTGCGACCGCTGTCTCCGGTTCACTTACCGGTATGC
TTGCGGCTAAAAAAATTCACGGGGTCACCGGAGATGTTTTAGGTGCATCAAGCGAGATGACAGAAGCGCTTGTCTGGTGT
TCAGCCCTCCTTCTGCTGTTCTACTGA

Upstream 100 bases:

>100_bases
CATTAGAGGTGATTCCCGATTGCGGACACCTTCCTCAGGAAGAGGCTCCTGAAGCTTTTGTTGCCGTCGTCGAATCCTTT
ATCGGGAGGGTCCGCTCATA

Downstream 100 bases:

>100_bases
TTGCTCGCTTTCCGTTTTTTTGTTCATGACGACGACGCGCTTTGTTGTTGGTAAATAGTGGGCATATGCTTATAATCACA
TTCTACTGATTGACTGTCTG

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLDGLVTAMRTLTVLSVPGKDADEFSRSLYWFPLVGLLLGLLQAALAWIGMVSQIPEFSALLVLLSGVLLTRAIHADGLA
DLADGFFGGKTRESRLRIMKDPAVGSFGVIALILLFLFKWIALTRIVAHGQYEWIVSGIVLARFVQVVLASVMTYAREGE
GTACRFVAGAGGWHVVVAALFSLLILVLVMKMQRLPIVVALLATAVSGSLTGMLAAKKIHGVTGDVLGASSEMTEALVWC
SALLLLFY

Sequences:

>Translated_248_residues
MLDGLVTAMRTLTVLSVPGKDADEFSRSLYWFPLVGLLLGLLQAALAWIGMVSQIPEFSALLVLLSGVLLTRAIHADGLA
DLADGFFGGKTRESRLRIMKDPAVGSFGVIALILLFLFKWIALTRIVAHGQYEWIVSGIVLARFVQVVLASVMTYAREGE
GTACRFVAGAGGWHVVVAALFSLLILVLVMKMQRLPIVVALLATAVSGSLTGMLAAKKIHGVTGDVLGASSEMTEALVWC
SALLLLFY
>Mature_248_residues
MLDGLVTAMRTLTVLSVPGKDADEFSRSLYWFPLVGLLLGLLQAALAWIGMVSQIPEFSALLVLLSGVLLTRAIHADGLA
DLADGFFGGKTRESRLRIMKDPAVGSFGVIALILLFLFKWIALTRIVAHGQYEWIVSGIVLARFVQVVLASVMTYAREGE
GTACRFVAGAGGWHVVVAALFSLLILVLVMKMQRLPIVVALLATAVSGSLTGMLAAKKIHGVTGDVLGASSEMTEALVWC
SALLLLFY

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=33.739837398374, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_PROA2 (B4S891)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015925.1
- GeneID:   6459375
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1252
- HOGENOM:   HBG729392
- OMA:   MQFALAS
- ProtClustDB:   CLSK637530
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26519; Mature: 26519

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d044e78)-; HASH(0x1d62a188)-; HASH(0x1dabcb6c)-; HASH(0x1d46b30c)-; HASH(0x1dabfc6c)-; HASH(0x1cb47cb8)-; HASH(0x1cd9d310)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDGLVTAMRTLTVLSVPGKDADEFSRSLYWFPLVGLLLGLLQAALAWIGMVSQIPEFSA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LLVLLSGVLLTRAIHADGLADLADGFFGGKTRESRLRIMKDPAVGSFGVIALILLFLFKW
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IALTRIVAHGQYEWIVSGIVLARFVQVVLASVMTYAREGEGTACRFVAGAGGWHVVVAAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
FSLLILVLVMKMQRLPIVVALLATAVSGSLTGMLAAKKIHGVTGDVLGASSEMTEALVWC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
SALLLLFY
HHHHHHHH
>Mature Secondary Structure
MLDGLVTAMRTLTVLSVPGKDADEFSRSLYWFPLVGLLLGLLQAALAWIGMVSQIPEFSA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LLVLLSGVLLTRAIHADGLADLADGFFGGKTRESRLRIMKDPAVGSFGVIALILLFLFKW
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IALTRIVAHGQYEWIVSGIVLARFVQVVLASVMTYAREGEGTACRFVAGAGGWHVVVAAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
FSLLILVLVMKMQRLPIVVALLATAVSGSLTGMLAAKKIHGVTGDVLGASSEMTEALVWC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
SALLLLFY
HHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA