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The map label for this gene is rpoN [H]

Identifier: 194333992

GI number: 194333992

Start: 1284730

End: 1286199

Strand: Reverse

Name: rpoN [H]

Synonym: Paes_1176

Alternate gene names: 194333992

Gene position: 1286199-1284730 (Counterclockwise)

Preceding gene: 194333993

Following gene: 194333991

Centisome position: 51.18

GC content: 48.03

Gene sequence:

>1470_bases
ATGCCAGACTTTAAGCTTCAGCAAAGCCAGAGGGCCCAGCTTTCTGCACAGCAGATTCTTTCGAGTCAGCTTCTGCAGCT
TCCCCTTCTTAACCTTGAGCAGCGGATATACGATGAGCTGCAGGAAAACCCGCTGCTTGAACTGATCGAAGAGAAGAACG
AATCGGTCGATGAAACAGGTGAGCAGAGCGGAGACGACGGCGATGACGGTGATGGAGAAGGGATGTTCGACTCTGTTGAT
CGTTTTGAAGCCAGGGATGAAAAGCGCCCTGAAAAGAACGAGCAGCCTAAAGAGAGCCAGGAAGGGGTATTGCATTTTAC
CGTCGATACGACACCCAAAGAGAATTTTATCCAGGCGATTCAGCAGGACTCTTTCGAAGAGCGAATGCTCAGAGATCTTT
CTCTCCGGGAGGAAATCGGTTCAACAGAACTGCTTATTGCATCGGAAATTCTCGGTAATCTTGATGATGACGGTTATCTT
CAGGACGGTCTGGATATTGTTGCCGATGGCTTGTTGACCAATCATGGCGTTGATGTTGAGCCGCGTGACATTCAACGCGT
GCTGCATATCATCCAGCGGATGGATCCCCCGGGGATTGCCGTAGCTTCGCTTCGCGAGCGTCTGCTCGTACAACTGGAAC
TGAACCAGGCAGCATCGGGATCAGGAGGGACAGCCATTATTGCCAAAAAGCTTCTTGACAGCTATTTTGATGATTTTCTC
AACAACCGCTATGAAAAAATCATCAAAAGGATGCAGATCAGGCCTGAACAGCTTGAAGCCGCGATTAAGGAGATTGCTGC
GCTGGATCCCCACCCTTTTGTCGTCTATCAGGATACGGGGGACTATATCATGCCTGATTTTATCGTGACCTATGAAAACG
GGCGTCTGACCGCAGCGCTCAATGATCGAAGTAATCTTTCTGTTCAGGTTTCGGACGCCTATCGCGACGTCCTGAAAAAC
AGGAAAATTCCACGAGGCGACAAGCAGTTTGTTCGTCAGAAACTTAACCGGGCGAAGGAATTCGCAAGTGCTATAGAGCA
GCGACGTCATACTTTGTTGAGCGTTATAGATGCTCTGATGCAGTTTCAGTACGAATTTTTTGTTTCAGGACCATCGATGC
TGGTTCCTCTGGGAATGAAGGATGTTGCGGAAAAATCCGGTTTTGATCTTTCAACGATAAGCAGAGCGGTCAACGGCAAA
TATGTTCAGACCCGTTTCGGGACCTTTGAGTTGAAATACTTTTTCAGTGGAGGGACAACGACGGACGAAGGCGAGGAGCT
CTCAACCAAGATCGTCAAACAGTACCTTCAGGAGATGGTAAGTGCTGAAGACTCAAAAAAACCTCTGAGCGATGACAGAC
TTGCACAGATGCTGGAGGAAAAAGGCATACGGATAGCCCGCAGAACGGTTGCAAAATACCGTGAACAAATGCAAATTCCA
GTAGCAAGGTTAAGAAAGAAAATATTTTAA

Upstream 100 bases:

>100_bases
CTTTCTGAAGGATGTGCTTGAAGATGCCTATGGAACCTCATTTTACAACCGTTTGAAGGATTTTTAGTACCATTAGTACC
AGTCAATACTCCCATGACCA

Downstream 100 bases:

>100_bases
TCATGAATTACAACGATGGGATAATCTCTCCCGAGGTGGCTTTAAACGGTCAGTTTTGCCGATTACCGTCGTCGATTGAT
TCGTTACTAATTTTATTACG

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MPDFKLQQSQRAQLSAQQILSSQLLQLPLLNLEQRIYDELQENPLLELIEEKNESVDETGEQSGDDGDDGDGEGMFDSVD
RFEARDEKRPEKNEQPKESQEGVLHFTVDTTPKENFIQAIQQDSFEERMLRDLSLREEIGSTELLIASEILGNLDDDGYL
QDGLDIVADGLLTNHGVDVEPRDIQRVLHIIQRMDPPGIAVASLRERLLVQLELNQAASGSGGTAIIAKKLLDSYFDDFL
NNRYEKIIKRMQIRPEQLEAAIKEIAALDPHPFVVYQDTGDYIMPDFIVTYENGRLTAALNDRSNLSVQVSDAYRDVLKN
RKIPRGDKQFVRQKLNRAKEFASAIEQRRHTLLSVIDALMQFQYEFFVSGPSMLVPLGMKDVAEKSGFDLSTISRAVNGK
YVQTRFGTFELKYFFSGGTTTDEGEELSTKIVKQYLQEMVSAEDSKKPLSDDRLAQMLEEKGIRIARRTVAKYREQMQIP
VARLRKKIF

Sequences:

>Translated_489_residues
MPDFKLQQSQRAQLSAQQILSSQLLQLPLLNLEQRIYDELQENPLLELIEEKNESVDETGEQSGDDGDDGDGEGMFDSVD
RFEARDEKRPEKNEQPKESQEGVLHFTVDTTPKENFIQAIQQDSFEERMLRDLSLREEIGSTELLIASEILGNLDDDGYL
QDGLDIVADGLLTNHGVDVEPRDIQRVLHIIQRMDPPGIAVASLRERLLVQLELNQAASGSGGTAIIAKKLLDSYFDDFL
NNRYEKIIKRMQIRPEQLEAAIKEIAALDPHPFVVYQDTGDYIMPDFIVTYENGRLTAALNDRSNLSVQVSDAYRDVLKN
RKIPRGDKQFVRQKLNRAKEFASAIEQRRHTLLSVIDALMQFQYEFFVSGPSMLVPLGMKDVAEKSGFDLSTISRAVNGK
YVQTRFGTFELKYFFSGGTTTDEGEELSTKIVKQYLQEMVSAEDSKKPLSDDRLAQMLEEKGIRIARRTVAKYREQMQIP
VARLRKKIF
>Mature_488_residues
PDFKLQQSQRAQLSAQQILSSQLLQLPLLNLEQRIYDELQENPLLELIEEKNESVDETGEQSGDDGDDGDGEGMFDSVDR
FEARDEKRPEKNEQPKESQEGVLHFTVDTTPKENFIQAIQQDSFEERMLRDLSLREEIGSTELLIASEILGNLDDDGYLQ
DGLDIVADGLLTNHGVDVEPRDIQRVLHIIQRMDPPGIAVASLRERLLVQLELNQAASGSGGTAIIAKKLLDSYFDDFLN
NRYEKIIKRMQIRPEQLEAAIKEIAALDPHPFVVYQDTGDYIMPDFIVTYENGRLTAALNDRSNLSVQVSDAYRDVLKNR
KIPRGDKQFVRQKLNRAKEFASAIEQRRHTLLSVIDALMQFQYEFFVSGPSMLVPLGMKDVAEKSGFDLSTISRAVNGKY
VQTRFGTFELKYFFSGGTTTDEGEELSTKIVKQYLQEMVSAEDSKKPLSDDRLAQMLEEKGIRIARRTVAKYREQMQIPV
ARLRKKIF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=501, Percent_Identity=29.940119760479, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55679; Mature: 55547

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFKLQQSQRAQLSAQQILSSQLLQLPLLNLEQRIYDELQENPLLELIEEKNESVDETG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHC
EQSGDDGDDGDGEGMFDSVDRFEARDEKRPEKNEQPKESQEGVLHFTVDTTPKENFIQAI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHH
QQDSFEERMLRDLSLREEIGSTELLIASEILGNLDDDGYLQDGLDIVADGLLTNHGVDVE
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PRDIQRVLHIIQRMDPPGIAVASLRERLLVQLELNQAASGSGGTAIIAKKLLDSYFDDFL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NNRYEKIIKRMQIRPEQLEAAIKEIAALDPHPFVVYQDTGDYIMPDFIVTYENGRLTAAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCEEEEECCCEEEEEE
NDRSNLSVQVSDAYRDVLKNRKIPRGDKQFVRQKLNRAKEFASAIEQRRHTLLSVIDALM
CCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFQYEFFVSGPSMLVPLGMKDVAEKSGFDLSTISRAVNGKYVQTRFGTFELKYFFSGGTT
HHHHHHEECCCCEEEECCHHHHHHHCCCCHHHHHHHHCCCEEEEECCEEEEEEEECCCCC
TDEGEELSTKIVKQYLQEMVSAEDSKKPLSDDRLAQMLEEKGIRIARRTVAKYREQMQIP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
VARLRKKIF
HHHHHHHCC
>Mature Secondary Structure 
PDFKLQQSQRAQLSAQQILSSQLLQLPLLNLEQRIYDELQENPLLELIEEKNESVDETG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHC
EQSGDDGDDGDGEGMFDSVDRFEARDEKRPEKNEQPKESQEGVLHFTVDTTPKENFIQAI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHH
QQDSFEERMLRDLSLREEIGSTELLIASEILGNLDDDGYLQDGLDIVADGLLTNHGVDVE
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PRDIQRVLHIIQRMDPPGIAVASLRERLLVQLELNQAASGSGGTAIIAKKLLDSYFDDFL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NNRYEKIIKRMQIRPEQLEAAIKEIAALDPHPFVVYQDTGDYIMPDFIVTYENGRLTAAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCEEEEECCCEEEEEE
NDRSNLSVQVSDAYRDVLKNRKIPRGDKQFVRQKLNRAKEFASAIEQRRHTLLSVIDALM
CCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFQYEFFVSGPSMLVPLGMKDVAEKSGFDLSTISRAVNGKYVQTRFGTFELKYFFSGGTT
HHHHHHEECCCCEEEECCHHHHHHHCCCCHHHHHHHHCCCEEEEECCEEEEEEEECCCCC
TDEGEELSTKIVKQYLQEMVSAEDSKKPLSDDRLAQMLEEKGIRIARRTVAKYREQMQIP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
VARLRKKIF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]