Click here to switch to the map view.

The map label for this gene is hslU [H]

Identifier: 194333990

GI number: 194333990

Start: 1282554

End: 1284017

Strand: Reverse

Name: hslU [H]

Synonym: Paes_1174

Alternate gene names: 194333990

Gene position: 1284017-1282554 (Counterclockwise)

Preceding gene: 194333991

Following gene: 194333989

Centisome position: 51.1

GC content: 50.2

Gene sequence:

>1464_bases
ATGATAGAGAAGACTGGTCATGAAGAGATGCATGACGACAGTACCGGGAGTATGACAGTAAAAAGTCTTATCAGCACCGA
ATCGCTGACTCCCAGCCAGATTGTCGATACGCTCGACAAATATATAATCGGCCAGCATGAGGCCAAGAAATCGGTAGCCA
TAGCGCTACGCAACAGACTTCGCCGCCAGAGTGTTGGTGAGGAACTGCGCGATGAGATTATGCCGAACAACATTATCATG
ATAGGTCCTACTGGTGTGGGAAAGACCGAGATTGCCCGCAGGCTTGCAAAGCTTTCAGGAGCGCCTTTCGTTAAAGTTGA
GGCATCGAAATTCACGGAAGTCGGTTACGTTGGACGTGATGTGGAATCTATGATACGTGATCTGACCGACCAGGCGGTCA
ATATGGTCCGTCATGAAAAGTCTGAGGAGGTCAGGGAAAAAGCTGCTGCTCTTGTTGAAGAACGCCTGCTCGATATTCTG
CTTCCGTCGGTTCCGGCTTCGTCTGGCGAAGATCGTTTGTCGGATGAAACCGCCAATGGCGAAGCGTTGTCGATGGAGGA
GGAACTCAATCGGAAAAGCAGGGAGAAAATGCTTGAACGCCTTCGTTCCGGGCGTATGGATGAGCGTCAGATTGAAATGG
AGATCAGCGGTGATTCCAATGGGGGTATGATGCAGATTTTCGGTCCGCTCGGTCAGATGGAGGAGATCGGCGGTATGATG
CAGGACCTTATGAACGGCCTGCCGAAAAAACGTAAAAAAAGGCGTGTGACGATTGCTGAAGCACGGAAGCTTCTCGAACA
GGAAGAGGTTCAGAAGCTGATCGATATGGACTCCGTTGTGAAGGAAGCGGTTCGGAAAGTTGAGGAGTCAGGGATCGTTT
TTATTGATGAGATCGATAAAATTGCCGCTCCGACCAGCGGTTCAGGCGGAAAAGGACCTGATGTCAGCAGGGAAGGGGTG
CAGCGTGATCTGCTTCCGATCGTCGAGGGTTCCAATGTTGCCACCAAGCACGGGATGGTGAAAACCGATCATGTGCTGTT
TATTGCTTCAGGAGCGTTTCATGTTGCCAAACCCTCTGACCTTATTCCCGAACTTCAGGGTCGATTTCCTATTCGCGTGG
AACTCAAAAGCCTTACCGAGGATGATTTTTACAGAATTCTTACCCAGCCGAAAAACGCCCTGATCAAGCAGTACAAGGCG
CTTATGGCTACCGAAGGCGTAGACCTGGAGTTCACTGATGATTCGATTCGTGAAATTGCCAGAACCGCGGCAAGAGTCAA
TGAGTCGGTTGAAAATATCGGAGCCCGGAGATTGCATACCATCATGACCAATCTCCTTGAGGAGCTGATGTTCAATATAC
CCGACAAGCACACCGATGGCAATGTCGTTATCGATGAGGCTGTCGTTTGCGGCAAAATGGACAGGCTCGTGAGCGATCGC
GACCTGAGCAGTTATATTCTCTGA

Upstream 100 bases:

>100_bases
ATCTGTATCTATACCAATGATCATATCGTCGTTGAAGAGGTCTGAGCAGAAGGCAATCCTTCTGTTTCAATCAAAAAAAT
CATAAAAACGCAGAAAACTG

Downstream 100 bases:

>100_bases
TTAAAAACGTTATTCATGATAGAACGAAATACTCTCAATCGAAGCGTTGCGGCACCGTCTGCTTCCGTGCTTCAGGATAC
TCAGACCCGGGTCATTAATC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MIEKTGHEEMHDDSTGSMTVKSLISTESLTPSQIVDTLDKYIIGQHEAKKSVAIALRNRLRRQSVGEELRDEIMPNNIIM
IGPTGVGKTEIARRLAKLSGAPFVKVEASKFTEVGYVGRDVESMIRDLTDQAVNMVRHEKSEEVREKAAALVEERLLDIL
LPSVPASSGEDRLSDETANGEALSMEEELNRKSREKMLERLRSGRMDERQIEMEISGDSNGGMMQIFGPLGQMEEIGGMM
QDLMNGLPKKRKKRRVTIAEARKLLEQEEVQKLIDMDSVVKEAVRKVEESGIVFIDEIDKIAAPTSGSGGKGPDVSREGV
QRDLLPIVEGSNVATKHGMVKTDHVLFIASGAFHVAKPSDLIPELQGRFPIRVELKSLTEDDFYRILTQPKNALIKQYKA
LMATEGVDLEFTDDSIREIARTAARVNESVENIGARRLHTIMTNLLEELMFNIPDKHTDGNVVIDEAVVCGKMDRLVSDR
DLSSYIL

Sequences:

>Translated_487_residues
MIEKTGHEEMHDDSTGSMTVKSLISTESLTPSQIVDTLDKYIIGQHEAKKSVAIALRNRLRRQSVGEELRDEIMPNNIIM
IGPTGVGKTEIARRLAKLSGAPFVKVEASKFTEVGYVGRDVESMIRDLTDQAVNMVRHEKSEEVREKAAALVEERLLDIL
LPSVPASSGEDRLSDETANGEALSMEEELNRKSREKMLERLRSGRMDERQIEMEISGDSNGGMMQIFGPLGQMEEIGGMM
QDLMNGLPKKRKKRRVTIAEARKLLEQEEVQKLIDMDSVVKEAVRKVEESGIVFIDEIDKIAAPTSGSGGKGPDVSREGV
QRDLLPIVEGSNVATKHGMVKTDHVLFIASGAFHVAKPSDLIPELQGRFPIRVELKSLTEDDFYRILTQPKNALIKQYKA
LMATEGVDLEFTDDSIREIARTAARVNESVENIGARRLHTIMTNLLEELMFNIPDKHTDGNVVIDEAVVCGKMDRLVSDR
DLSSYIL
>Mature_487_residues
MIEKTGHEEMHDDSTGSMTVKSLISTESLTPSQIVDTLDKYIIGQHEAKKSVAIALRNRLRRQSVGEELRDEIMPNNIIM
IGPTGVGKTEIARRLAKLSGAPFVKVEASKFTEVGYVGRDVESMIRDLTDQAVNMVRHEKSEEVREKAAALVEERLLDIL
LPSVPASSGEDRLSDETANGEALSMEEELNRKSREKMLERLRSGRMDERQIEMEISGDSNGGMMQIFGPLGQMEEIGGMM
QDLMNGLPKKRKKRRVTIAEARKLLEQEEVQKLIDMDSVVKEAVRKVEESGIVFIDEIDKIAAPTSGSGGKGPDVSREGV
QRDLLPIVEGSNVATKHGMVKTDHVLFIASGAFHVAKPSDLIPELQGRFPIRVELKSLTEDDFYRILTQPKNALIKQYKA
LMATEGVDLEFTDDSIREIARTAARVNESVENIGARRLHTIMTNLLEELMFNIPDKHTDGNVVIDEAVVCGKMDRLVSDR
DLSSYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=112, Percent_Identity=41.9642857142857, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1790366, Length=460, Percent_Identity=54.5652173913043, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1786642, Length=279, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=38.2113821138211, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648291, Length=251, Percent_Identity=32.2709163346614, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24648289, Length=251, Percent_Identity=32.2709163346614, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 54237; Mature: 54237

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKTGHEEMHDDSTGSMTVKSLISTESLTPSQIVDTLDKYIIGQHEAKKSVAIALRNRL
CCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RRQSVGEELRDEIMPNNIIMIGPTGVGKTEIARRLAKLSGAPFVKVEASKFTEVGYVGRD
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCC
VESMIRDLTDQAVNMVRHEKSEEVREKAAALVEERLLDILLPSVPASSGEDRLSDETANG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
EALSMEEELNRKSREKMLERLRSGRMDERQIEMEISGDSNGGMMQIFGPLGQMEEIGGMM
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
QDLMNGLPKKRKKRRVTIAEARKLLEQEEVQKLIDMDSVVKEAVRKVEESGIVFIDEIDK
HHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHH
IAAPTSGSGGKGPDVSREGVQRDLLPIVEGSNVATKHGMVKTDHVLFIASGAFHVAKPSD
HCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEECCEEEEECCCEECCCCHH
LIPELQGRFPIRVELKSLTEDDFYRILTQPKNALIKQYKALMATEGVDLEFTDDSIREIA
HHHHHCCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHH
RTAARVNESVENIGARRLHTIMTNLLEELMFNIPDKHTDGNVVIDEAVVCGKMDRLVSDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCC
DLSSYIL
CHHHHCC
>Mature Secondary Structure
MIEKTGHEEMHDDSTGSMTVKSLISTESLTPSQIVDTLDKYIIGQHEAKKSVAIALRNRL
CCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RRQSVGEELRDEIMPNNIIMIGPTGVGKTEIARRLAKLSGAPFVKVEASKFTEVGYVGRD
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCC
VESMIRDLTDQAVNMVRHEKSEEVREKAAALVEERLLDILLPSVPASSGEDRLSDETANG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
EALSMEEELNRKSREKMLERLRSGRMDERQIEMEISGDSNGGMMQIFGPLGQMEEIGGMM
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
QDLMNGLPKKRKKRRVTIAEARKLLEQEEVQKLIDMDSVVKEAVRKVEESGIVFIDEIDK
HHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHH
IAAPTSGSGGKGPDVSREGVQRDLLPIVEGSNVATKHGMVKTDHVLFIASGAFHVAKPSD
HCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEECCEEEEECCCEECCCCHH
LIPELQGRFPIRVELKSLTEDDFYRILTQPKNALIKQYKALMATEGVDLEFTDDSIREIA
HHHHHCCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHH
RTAARVNESVENIGARRLHTIMTNLLEELMFNIPDKHTDGNVVIDEAVVCGKMDRLVSDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCC
DLSSYIL
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]