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The map label for this gene is argF [H]

Identifier: 194333895

GI number: 194333895

Start: 1181739

End: 1182773

Strand: Reverse

Name: argF [H]

Synonym: Paes_1073

Alternate gene names: 194333895

Gene position: 1182773-1181739 (Counterclockwise)

Preceding gene: 194333896

Following gene: 194333894

Centisome position: 47.07

GC content: 49.86

Gene sequence:

>1035_bases
ATGACATCATCCATGCCCCATCCAACGAAAGAAACAATAGTCAAAAAAAGGGATTTCCTCGGATTTCAGCCTCTCGATGC
AGGAAAGATTATTGAGCTGTTTGATTTTTCAGCCTTTATCAAGGCAAAACGGCTCGACAGAACTGCGGCCAGCAAATACA
AGCCGATAGAAGGCAAAACCGTTGCCATGATTTTCAACAAGCCTTCGCTGCGCACAAGGGTTTCTTTCGAGCTCGGCATT
CATGAGCTTGGGGGGTATGCAGTCAATCTCGATGGAAAGTCAATCGGTGTCAATTCGCGCGAAGCCGTTGAAGATATCGC
ACAACTGCTTTCGCGATATAACGACGCTATCGTAGCCCGTCTGCATGAGCATTCGGTCATCGAAGACCTTGCTGCCAACG
CAACGATTCCGGTCATCAATGCACTGACTGACCTGTCGCATCCCTGTCAGATTCTGGCGGATGCCTTTACACTCTACGAA
AAAGGTCTCTGGAAAGAGGGGGTCAAGATAGTGTTCGTCGGAGACGGCAACAATGTGGCCAATTCATGGATTGAACTTGC
CGGTATTCTTCCTTTTCACTTTGTGCTCGCCTGCCCTCAGAACGAGGAATACATGCCTGACACAACACTTCTCGAAGAAG
CACGTCGTAACTCCGGGAGCACGATAGAGATCGTCCATGATCCCATGGAAGCAGCAAAAAATGCCGATGTACTCTATACC
GATGTCTGGACAAGTATGGGCCAGGAGGATGAGGTTGCCGAGCGGCTCAAAGCGTTCAGCAGCTACCAGATCAACAGCGC
TATGCTTCGCCAGTCAAAACCCTCCGCTGTCGTCATGCATTGTATGCCGGCTCACCGCGGGCAGGAAATAAGCGCAGAGG
TTATGGACGGGCCGCAATCGATTATCATGGACGAAGCAGAAAACCGCCTGCATGTTCAAAAAGCGTTGCTCGTCAAGTTG
CTCAACCATGAAGAGTACAGGAAATTTCACCTGACCCATCGCCTTGAACATGTCGCAAAAAACCTGAAGCACTGA

Upstream 100 bases:

>100_bases
TCGACGGACGATTTGTGCACTCCCTCCTGCTGGAAATCTTTACCAACGCAGGCGTCGGCACACAGTTTGTATCTGAAAAA
GAGTCTCAATAATCGTAAAC

Downstream 100 bases:

>100_bases
GATTATGGGCAAACAGCTCAGACAGATGAAGATCAAGGAGATCCTGCACAACCATGATGTCGGCAACCAGAACGACCTGA
TCAGGCTGCTCGACAAAGCC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTSSMPHPTKETIVKKRDFLGFQPLDAGKIIELFDFSAFIKAKRLDRTAASKYKPIEGKTVAMIFNKPSLRTRVSFELGI
HELGGYAVNLDGKSIGVNSREAVEDIAQLLSRYNDAIVARLHEHSVIEDLAANATIPVINALTDLSHPCQILADAFTLYE
KGLWKEGVKIVFVGDGNNVANSWIELAGILPFHFVLACPQNEEYMPDTTLLEEARRNSGSTIEIVHDPMEAAKNADVLYT
DVWTSMGQEDEVAERLKAFSSYQINSAMLRQSKPSAVVMHCMPAHRGQEISAEVMDGPQSIIMDEAENRLHVQKALLVKL
LNHEEYRKFHLTHRLEHVAKNLKH

Sequences:

>Translated_344_residues
MTSSMPHPTKETIVKKRDFLGFQPLDAGKIIELFDFSAFIKAKRLDRTAASKYKPIEGKTVAMIFNKPSLRTRVSFELGI
HELGGYAVNLDGKSIGVNSREAVEDIAQLLSRYNDAIVARLHEHSVIEDLAANATIPVINALTDLSHPCQILADAFTLYE
KGLWKEGVKIVFVGDGNNVANSWIELAGILPFHFVLACPQNEEYMPDTTLLEEARRNSGSTIEIVHDPMEAAKNADVLYT
DVWTSMGQEDEVAERLKAFSSYQINSAMLRQSKPSAVVMHCMPAHRGQEISAEVMDGPQSIIMDEAENRLHVQKALLVKL
LNHEEYRKFHLTHRLEHVAKNLKH
>Mature_343_residues
TSSMPHPTKETIVKKRDFLGFQPLDAGKIIELFDFSAFIKAKRLDRTAASKYKPIEGKTVAMIFNKPSLRTRVSFELGIH
ELGGYAVNLDGKSIGVNSREAVEDIAQLLSRYNDAIVARLHEHSVIEDLAANATIPVINALTDLSHPCQILADAFTLYEK
GLWKEGVKIVFVGDGNNVANSWIELAGILPFHFVLACPQNEEYMPDTTLLEEARRNSGSTIEIVHDPMEAAKNADVLYTD
VWTSMGQEDEVAERLKAFSSYQINSAMLRQSKPSAVVMHCMPAHRGQEISAEVMDGPQSIIMDEAENRLHVQKALLVKLL
NHEEYRKFHLTHRLEHVAKNLKH

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=36.741214057508, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI18105007, Length=277, Percent_Identity=31.4079422382672, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1790703, Length=330, Percent_Identity=33.030303030303, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=32.4324324324324, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=28.9719626168224, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=26.5060240963855, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=302, Percent_Identity=30.4635761589404, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322331, Length=271, Percent_Identity=29.1512915129151, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24642586, Length=300, Percent_Identity=26.3333333333333, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 38542; Mature: 38410

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSMPHPTKETIVKKRDFLGFQPLDAGKIIELFDFSAFIKAKRLDRTAASKYKPIEGKT
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VAMIFNKPSLRTRVSFELGIHELGGYAVNLDGKSIGVNSREAVEDIAQLLSRYNDAIVAR
EEEEECCCCCCEEEEEEECHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHEHSVIEDLAANATIPVINALTDLSHPCQILADAFTLYEKGLWKEGVKIVFVGDGNNVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
NSWIELAGILPFHFVLACPQNEEYMPDTTLLEEARRNSGSTIEIVHDPMEAAKNADVLYT
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEE
DVWTSMGQEDEVAERLKAFSSYQINSAMLRQSKPSAVVMHCMPAHRGQEISAEVMDGPQS
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCHH
IIMDEAENRLHVQKALLVKLLNHEEYRKFHLTHRLEHVAKNLKH
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSMPHPTKETIVKKRDFLGFQPLDAGKIIELFDFSAFIKAKRLDRTAASKYKPIEGKT
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VAMIFNKPSLRTRVSFELGIHELGGYAVNLDGKSIGVNSREAVEDIAQLLSRYNDAIVAR
EEEEECCCCCCEEEEEEECHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHEHSVIEDLAANATIPVINALTDLSHPCQILADAFTLYEKGLWKEGVKIVFVGDGNNVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
NSWIELAGILPFHFVLACPQNEEYMPDTTLLEEARRNSGSTIEIVHDPMEAAKNADVLYT
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEE
DVWTSMGQEDEVAERLKAFSSYQINSAMLRQSKPSAVVMHCMPAHRGQEISAEVMDGPQS
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCHH
IIMDEAENRLHVQKALLVKLLNHEEYRKFHLTHRLEHVAKNLKH
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]