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The map label for this gene is argG

Identifier: 194333893

GI number: 194333893

Start: 1180065

End: 1181270

Strand: Reverse

Name: argG

Synonym: Paes_1071

Alternate gene names: 194333893

Gene position: 1181270-1180065 (Counterclockwise)

Preceding gene: 194333894

Following gene: 194333892

Centisome position: 47.01

GC content: 52.74

Gene sequence:

>1206_bases
ATGAGCAAGGAAAAAATCGCTCTCGCCTATTCCGGCGGACTCGACACCTCGGTGATGATCAAATGGCTTAAAGACAAATA
TGATGCCGAGATCGTGGCTGTCACAGGAAACCTCGGCCAGGAAAAAGAGGTGGAAAACCTCGAAGAAAAAGCTATCGCAA
CCGGAGCATCCAGCTTCGCTTTTCTCGATCTTCGCAAAGAATTTGTAGAAAGCTGCATCTGGCCGGCCCTGAAAGCAGGT
GCGCTCTACGAGGATGTCTATCCCCTGGCTACAGCGCTCGGTCGCCCGCTGATTGCAAAAGCGCTCGTCGATATCGCGCT
GGAAAACAACTGCACCATGCTCGCTCACGGCTGCACCGGCAAAGGCAATGATCAGGTTCGTTTCGAGGTCACCTTCGCCT
CACTCGCTCCACAGCTTGCCGTGCTGGCACCGTTGCGTGAATGGGAGTTCACGTCACGCGAAGCAGAAATCGCCTACGCA
ATGGAGCATAACATCCCGGTTTCAGCCACCAAGAAAAGCCCATACTCTATCGATGAAAACATCTGGGGAATCAGCATTGA
GTGCGGTGTTCTCGAAGATCCGATGACGCCGGCCCCTGAAGATGCCTATCAGATCACAACGTCACCTGAAAAAGCGCCAG
ACAAGGCTGCTGTGATTGACATTGAATTTGAACAGGGTGTTCCTGTCGCACTGGACGGAAAAGCCATGGAGGGTCTTGAC
CTCATCGTTGAGCTCAACAAGGTAGGCGCAGCTCATGGCGTAGGCCGTCTCGATATGGTCGAAAACCGTGTTGTCGGCAT
CAAATCCAGAGAGATCTACGAAGCCCCGGCAGCAACCATCCTGCACTTCGCACACCGTGAGCTCGAACGCCTGACCCTGG
AAAAATCGGTCTTCCAGTACAAGAAAAACGTCAGTCAGGATTACGCCAACCTGATCTACAACGGCACCTGGTTTTCGCCG
ATGCGTGAAGCGCTTGACGGATTTATCGAAGCTACACAGAAAACGGTCACCGGCCTTGTTCGCGTCAAACTCTTCAAAGG
CTCGGTAACATTGCTCGGCAGAACCTCGCCATGGTCACTCTACAATGAGGATCTGGCAACCTATACGGAAGCCGATACCT
TCAACCACAAAGCAGCCGAAGGCTTCATCCACCTCTACGGTCTCGGTCTGAAAACATGGAGTGAAGTCAAAGCCAATAAC
AGCTGA

Upstream 100 bases:

>100_bases
ACACAGTCCTTGTTATTCCCCTCTCCATTAAGGATATTGCATCCATTATCCATGACATTCAGTCTAATCTATCACAAACC
TGATAACACAACACACACGT

Downstream 100 bases:

>100_bases
TGATTTCCGCTTACGCTTTCCCCCGGCCCAATGCCGGGGGAATCGTATTGACCACGTTTCACCTGCGCATCCGATCAAAC
ACAGAACACCATCCCCAAAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSKEKIALAYSGGLDTSVMIKWLKDKYDAEIVAVTGNLGQEKEVENLEEKAIATGASSFAFLDLRKEFVESCIWPALKAG
ALYEDVYPLATALGRPLIAKALVDIALENNCTMLAHGCTGKGNDQVRFEVTFASLAPQLAVLAPLREWEFTSREAEIAYA
MEHNIPVSATKKSPYSIDENIWGISIECGVLEDPMTPAPEDAYQITTSPEKAPDKAAVIDIEFEQGVPVALDGKAMEGLD
LIVELNKVGAAHGVGRLDMVENRVVGIKSREIYEAPAATILHFAHRELERLTLEKSVFQYKKNVSQDYANLIYNGTWFSP
MREALDGFIEATQKTVTGLVRVKLFKGSVTLLGRTSPWSLYNEDLATYTEADTFNHKAAEGFIHLYGLGLKTWSEVKANN
S

Sequences:

>Translated_401_residues
MSKEKIALAYSGGLDTSVMIKWLKDKYDAEIVAVTGNLGQEKEVENLEEKAIATGASSFAFLDLRKEFVESCIWPALKAG
ALYEDVYPLATALGRPLIAKALVDIALENNCTMLAHGCTGKGNDQVRFEVTFASLAPQLAVLAPLREWEFTSREAEIAYA
MEHNIPVSATKKSPYSIDENIWGISIECGVLEDPMTPAPEDAYQITTSPEKAPDKAAVIDIEFEQGVPVALDGKAMEGLD
LIVELNKVGAAHGVGRLDMVENRVVGIKSREIYEAPAATILHFAHRELERLTLEKSVFQYKKNVSQDYANLIYNGTWFSP
MREALDGFIEATQKTVTGLVRVKLFKGSVTLLGRTSPWSLYNEDLATYTEADTFNHKAAEGFIHLYGLGLKTWSEVKANN
S
>Mature_400_residues
SKEKIALAYSGGLDTSVMIKWLKDKYDAEIVAVTGNLGQEKEVENLEEKAIATGASSFAFLDLRKEFVESCIWPALKAGA
LYEDVYPLATALGRPLIAKALVDIALENNCTMLAHGCTGKGNDQVRFEVTFASLAPQLAVLAPLREWEFTSREAEIAYAM
EHNIPVSATKKSPYSIDENIWGISIECGVLEDPMTPAPEDAYQITTSPEKAPDKAAVIDIEFEQGVPVALDGKAMEGLDL
IVELNKVGAAHGVGRLDMVENRVVGIKSREIYEAPAATILHFAHRELERLTLEKSVFQYKKNVSQDYANLIYNGTWFSPM
REALDGFIEATQKTVTGLVRVKLFKGSVTLLGRTSPWSLYNEDLATYTEADTFNHKAAEGFIHLYGLGLKTWSEVKANNS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=44.2260442260442, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=44.2260442260442, Blast_Score=335, Evalue=6e-92,
Organism=Escherichia coli, GI1789563, Length=395, Percent_Identity=30.6329113924051, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324514, Length=412, Percent_Identity=42.4757281553398, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=41.2776412776413, Blast_Score=304, Evalue=6e-83,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PROA2 (B4S7R9)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015753.1
- ProteinModelPortal:   B4S7R9
- SMR:   B4S7R9
- GeneID:   6459484
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1071
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44135; Mature: 44003

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 176-176 BINDING 185-185 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKIALAYSGGLDTSVMIKWLKDKYDAEIVAVTGNLGQEKEVENLEEKAIATGASSFA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHH
FLDLRKEFVESCIWPALKAGALYEDVYPLATALGRPLIAKALVDIALENNCTMLAHGCTG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCC
KGNDQVRFEVTFASLAPQLAVLAPLREWEFTSREAEIAYAMEHNIPVSATKKSPYSIDEN
CCCCEEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCC
IWGISIECGVLEDPMTPAPEDAYQITTSPEKAPDKAAVIDIEFEQGVPVALDGKAMEGLD
EEEEEEECCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEECCCCCCCEE
LIVELNKVGAAHGVGRLDMVENRVVGIKSREIYEAPAATILHFAHRELERLTLEKSVFQY
EEEEEHHHCCCCCCCCHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
KKNVSQDYANLIYNGTWFSPMREALDGFIEATQKTVTGLVRVKLFKGSVTLLGRTSPWSL
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCCCCH
YNEDLATYTEADTFNHKAAEGFIHLYGLGLKTWSEVKANNS
HCCHHHHHCCCCCCCCHHHCCEEEEEECCCCCHHHHCCCCC
>Mature Secondary Structure 
SKEKIALAYSGGLDTSVMIKWLKDKYDAEIVAVTGNLGQEKEVENLEEKAIATGASSFA
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHH
FLDLRKEFVESCIWPALKAGALYEDVYPLATALGRPLIAKALVDIALENNCTMLAHGCTG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCC
KGNDQVRFEVTFASLAPQLAVLAPLREWEFTSREAEIAYAMEHNIPVSATKKSPYSIDEN
CCCCEEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCC
IWGISIECGVLEDPMTPAPEDAYQITTSPEKAPDKAAVIDIEFEQGVPVALDGKAMEGLD
EEEEEEECCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEECCCCCCCEE
LIVELNKVGAAHGVGRLDMVENRVVGIKSREIYEAPAATILHFAHRELERLTLEKSVFQY
EEEEEHHHCCCCCCCCHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
KKNVSQDYANLIYNGTWFSPMREALDGFIEATQKTVTGLVRVKLFKGSVTLLGRTSPWSL
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCCCCH
YNEDLATYTEADTFNHKAAEGFIHLYGLGLKTWSEVKANNS
HCCHHHHHCCCCCCCCHHHCCEEEEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA