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The map label for this gene is nadE2 [H]

Identifier: 193213226

GI number: 193213226

Start: 1730631

End: 1731491

Strand: Direct

Name: nadE2 [H]

Synonym: Cpar_1581

Alternate gene names: 193213226

Gene position: 1730631-1731491 (Clockwise)

Preceding gene: 193213225

Following gene: 193213228

Centisome position: 75.6

GC content: 58.77

Gene sequence:

>861_bases
ATGCTCAAAGCCAAGCTCAGAATCGTTCAGTCGGACTGCACGCTCGCCAATTTCGACGAGAACCTCGAAAGCCACGTCAA
GGCGATCGAACAGGCCATCAGGGATGGAGTCGAGGCGATCGTTTTTCCTGAACTCTCACTCACCGGCTATAACGTGCAGG
ATGCCGCACAGGACATGGCGATGCACATCGAGGACCACAGGCTCGCCCCGCTTCGGGAGCTGAGCCGCGAAATTACGGTT
ATCTGTGGGGGAATCGAGCTGAGTGACGACTACGGAGTCTATAATTCGGCATTCATGTTCGAGGACGGAATAGGCAAGAG
CGTGCACCGCAAAATCTACCTGCCGACCTACGGCATGTTCGAGGAGCTGCGCTATTTTTCGGCGGGACGTCAGGTCGAGG
CTGTGCCGTCACGGCGGCTCGGAAAAATCGGCGTGGCAATCTGCGAGGATTTCTGGCATATGTCGGTGCCCTATCTGCTG
GCCCATCAGGGCGCGAAGCTGCTGCTGGTGCTGATGTCGAGCCCGCTCCGGCTTTCGCCCGGCGAGGGTATGCCCTCCAT
CGTCTCGCAGTGGCAAACCATCGCTTCGACCAGCGCGTTTTTGTTCAGCTGCTACGTGGCCTGCGTCAACCGGGTGGGCA
ACGAAGACAGCTTCACCTACTGGGGCAACTCTGCACTCACCGCGCCGGACGGTTCAAACGTTGCCAGCGCACCGATGTTC
AGCGAACACCAGCTTGACGCACTGATCGACTATTCACTGGTCAAGCGGGTACGCCTCCAGTCCTCGCACTTCCTCGACGA
GGACATCAAGCTGTTTGCCTCGGAGCTGGAAGATCTGATGAGCGCGAAGCGACGGGGGTGA

Upstream 100 bases:

>100_bases
TCTCGGGCAGGTTCAGCGAAATCAACACAGGCATGGCGGTGCTGGCCGCGCTGTCGCTGACCTTCTACCTCTTCTATCCC
TACCACTAAGACCTGAAACC

Downstream 100 bases:

>100_bases
ATACCTGGACTATTGCTCAGACGATTAAATATCTTGAACAATTGCCCCGGACTTCAGTCCGGGGTTCATGGATAAACAAA
AAAAATCAGGGCTTCAGCCC

Product: nitrilase/cyanide hydratase and apolipoprotein n-acyltransferase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLKAKLRIVQSDCTLANFDENLESHVKAIEQAIRDGVEAIVFPELSLTGYNVQDAAQDMAMHIEDHRLAPLRELSREITV
ICGGIELSDDYGVYNSAFMFEDGIGKSVHRKIYLPTYGMFEELRYFSAGRQVEAVPSRRLGKIGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGEGMPSIVSQWQTIASTSAFLFSCYVACVNRVGNEDSFTYWGNSALTAPDGSNVASAPMF
SEHQLDALIDYSLVKRVRLQSSHFLDEDIKLFASELEDLMSAKRRG

Sequences:

>Translated_286_residues
MLKAKLRIVQSDCTLANFDENLESHVKAIEQAIRDGVEAIVFPELSLTGYNVQDAAQDMAMHIEDHRLAPLRELSREITV
ICGGIELSDDYGVYNSAFMFEDGIGKSVHRKIYLPTYGMFEELRYFSAGRQVEAVPSRRLGKIGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGEGMPSIVSQWQTIASTSAFLFSCYVACVNRVGNEDSFTYWGNSALTAPDGSNVASAPMF
SEHQLDALIDYSLVKRVRLQSSHFLDEDIKLFASELEDLMSAKRRG
>Mature_286_residues
MLKAKLRIVQSDCTLANFDENLESHVKAIEQAIRDGVEAIVFPELSLTGYNVQDAAQDMAMHIEDHRLAPLRELSREITV
ICGGIELSDDYGVYNSAFMFEDGIGKSVHRKIYLPTYGMFEELRYFSAGRQVEAVPSRRLGKIGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGEGMPSIVSQWQTIASTSAFLFSCYVACVNRVGNEDSFTYWGNSALTAPDGSNVASAPMF
SEHQLDALIDYSLVKRVRLQSSHFLDEDIKLFASELEDLMSAKRRG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31884; Mature: 31884

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAKLRIVQSDCTLANFDENLESHVKAIEQAIRDGVEAIVFPELSLTGYNVQDAAQDMA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHHHH
MHIEDHRLAPLRELSREITVICGGIELSDDYGVYNSAFMFEDGIGKSVHRKIYLPTYGMF
HHHCCCCCCHHHHHHHCEEEEECCEEECCCCCCCCCEEEECCCCCCCHHCEEECCCHHHH
EELRYFSAGRQVEAVPSRRLGKIGVAICEDFWHMSVPYLLAHQGAKLLLVLMSSPLRLSP
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEHHHHCCCCEECC
GEGMPSIVSQWQTIASTSAFLFSCYVACVNRVGNEDSFTYWGNSALTAPDGSNVASAPMF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCCCC
SEHQLDALIDYSLVKRVRLQSSHFLDEDIKLFASELEDLMSAKRRG
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKAKLRIVQSDCTLANFDENLESHVKAIEQAIRDGVEAIVFPELSLTGYNVQDAAQDMA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHHHH
MHIEDHRLAPLRELSREITVICGGIELSDDYGVYNSAFMFEDGIGKSVHRKIYLPTYGMF
HHHCCCCCCHHHHHHHCEEEEECCEEECCCCCCCCCEEEECCCCCCCHHCEEECCCHHHH
EELRYFSAGRQVEAVPSRRLGKIGVAICEDFWHMSVPYLLAHQGAKLLLVLMSSPLRLSP
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEHHHHCCCCEECC
GEGMPSIVSQWQTIASTSAFLFSCYVACVNRVGNEDSFTYWGNSALTAPDGSNVASAPMF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCCCC
SEHQLDALIDYSLVKRVRLQSSHFLDEDIKLFASELEDLMSAKRRG
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]