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The map label for this gene is mpl [H]

Identifier: 193213214

GI number: 193213214

Start: 1717686

End: 1719110

Strand: Direct

Name: mpl [H]

Synonym: Cpar_1569

Alternate gene names: 193213214

Gene position: 1717686-1719110 (Clockwise)

Preceding gene: 193213212

Following gene: 193213215

Centisome position: 75.03

GC content: 60.0

Gene sequence:

>1425_bases
ATGAGTTCCATCTATTTTATCGGCATCGGCGGCTCGGCAATGGCTTCGGTGGCCGTGGCGCTTTCGCACATGGGCCACGC
CGTTACCGGTTCGGATACGCAGCTCTATCCTCCGATGAGCACCTATCTTGAAAATCATAACATCCGCTATTTCAACGACT
TTTCTGCAGAGAACCTGAAAAGCGCATCGCCCGATCTGGTGGTGGTTGGCAATGCGATCAGCCGGGGCAATCCCGATCTT
GAGTACGCGCTCGACACGCACATGGAGCTGATTTCGATGCCGGAGCTGGTGCGGCGCGAGCTGATTGGACGCCACACCTC
GATTGTGGTGGCAGGCACGCACGGCAAGACAACGACCACTTCCCTCGCCGCATGGTTGCTCGAAGCGGGCGGCTTTCTGC
CGGGCTTCCTCATCGGCGGCATTCCCGAGAATTTCGGCGACGGCTGCCGCCCTTCCGGCCTCTCCGAGCCGGGCTTTTTC
GTGACCGAGGGCGACGAGTACGACTCGGCCTTTTTCGACAAGCGCAGCAAGTTCCTGCATTACCGCCCCGACATCGCGAT
CATCAACAACGTCGAGTTCGACCACGCGGACATCTTCGACTCGCTCGACGACATCAAGAAGAGCTTCTGCCTGCTGGTGA
ACCTCGTGCCGTCGAGCGGCCTCTTGATCGTCAACGGCGACGATCCGAATTCGATGGAGGTCGCCGCGAAAGCGTTCTGC
CGCGTCGAGACCTTTGGCATGAACGGCACAGCGGACTGGACGGCAACCGACATCACTACCGACGCCGAAAGCACCGTCTT
CACGGTCGTACACAATGGCGAAGCGATGGGCCGGGTAAGCGTGCCGCTTTTCGGCAACTACAACGTGATGAACGCGCTGG
CAGCCACGGCGGCTGCAATCCGCGCGGGCGTGAGCTTTGAGTCGATCACCAAAGGGCTTGCAAAATTCAAACGCCCGAAG
CGCCGCATGGAGCTGGTCGGTGAGTACGCAGGCGGCGTAACGCTCATCGAGGACTTCGCGCATCACCCGACGGCCGTACG
GCTCACGCTCGGTGCGATTGCGGAACACTACCCCGGCAGGCGCATCGTGGCCTGTTTCGAGCCTCGCTCCAACACCACCA
CGCGCAACATCTTTCAGCACGAGCTGACGGAGTGCTTCGACGACGCTTCGGTCGTGGTTCTCGGCAGGGTGAACCGGCCT
GATCGCTACGCCCCCGAAAAACGGCTCGATGCGGCGCAGCTTTGCCACGAGCTGGAAGCAAAAGGCAAGCGTGTCTTCGC
GGCTGGCGACGACGACTATCCGAACGACATCGTCCAGTTCATCAAAGCAGAACAGCAGCCCGGCGACGTGGTTGTACTGC
TGAGCAACGGCAGCTTCAACGGCCTGAAAACGCTGCTGGCGGAAAGTTTTGAAAAAAAATCATGA

Upstream 100 bases:

>100_bases
AAGTTCGGACAGAGTACGGTCTGTTTTGCGAGGCTGGGGTTTTTCTGTACTATTGGACTTAAGGGTATACATCACAAGCA
CCTGACCAACATTCCCAACC

Downstream 100 bases:

>100_bases
AGATTTCATTGGCGATAGGCTATTTTTCTATCTTGTGACTTTTCCTGGGCGGCGCATGCCCGGTTCATGCCGCTGCAACC
ATCCCGAACAGAGTCATTAT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNDFSAENLKSASPDLVVVGNAISRGNPDL
EYALDTHMELISMPELVRRELIGRHTSIVVAGTHGKTTTTSLAAWLLEAGGFLPGFLIGGIPENFGDGCRPSGLSEPGFF
VTEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFCLLVNLVPSSGLLIVNGDDPNSMEVAAKAFC
RVETFGMNGTADWTATDITTDAESTVFTVVHNGEAMGRVSVPLFGNYNVMNALAATAAAIRAGVSFESITKGLAKFKRPK
RRMELVGEYAGGVTLIEDFAHHPTAVRLTLGAIAEHYPGRRIVACFEPRSNTTTRNIFQHELTECFDDASVVVLGRVNRP
DRYAPEKRLDAAQLCHELEAKGKRVFAAGDDDYPNDIVQFIKAEQQPGDVVVLLSNGSFNGLKTLLAESFEKKS

Sequences:

>Translated_474_residues
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNDFSAENLKSASPDLVVVGNAISRGNPDL
EYALDTHMELISMPELVRRELIGRHTSIVVAGTHGKTTTTSLAAWLLEAGGFLPGFLIGGIPENFGDGCRPSGLSEPGFF
VTEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFCLLVNLVPSSGLLIVNGDDPNSMEVAAKAFC
RVETFGMNGTADWTATDITTDAESTVFTVVHNGEAMGRVSVPLFGNYNVMNALAATAAAIRAGVSFESITKGLAKFKRPK
RRMELVGEYAGGVTLIEDFAHHPTAVRLTLGAIAEHYPGRRIVACFEPRSNTTTRNIFQHELTECFDDASVVVLGRVNRP
DRYAPEKRLDAAQLCHELEAKGKRVFAAGDDDYPNDIVQFIKAEQQPGDVVVLLSNGSFNGLKTLLAESFEKKS
>Mature_473_residues
SSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNDFSAENLKSASPDLVVVGNAISRGNPDLE
YALDTHMELISMPELVRRELIGRHTSIVVAGTHGKTTTTSLAAWLLEAGGFLPGFLIGGIPENFGDGCRPSGLSEPGFFV
TEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFCLLVNLVPSSGLLIVNGDDPNSMEVAAKAFCR
VETFGMNGTADWTATDITTDAESTVFTVVHNGEAMGRVSVPLFGNYNVMNALAATAAAIRAGVSFESITKGLAKFKRPKR
RMELVGEYAGGVTLIEDFAHHPTAVRLTLGAIAEHYPGRRIVACFEPRSNTTTRNIFQHELTECFDDASVVVLGRVNRPD
RYAPEKRLDAAQLCHELEAKGKRVFAAGDDDYPNDIVQFIKAEQQPGDVVVLLSNGSFNGLKTLLAESFEKKS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=472, Percent_Identity=40.8898305084746, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1786279, Length=430, Percent_Identity=30, Blast_Score=144, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51548; Mature: 51417

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNDFSAENLK
CCEEEEEEECHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHHCCCCEEEEECCCCHHHC
SASPDLVVVGNAISRGNPDLEYALDTHMELISMPELVRRELIGRHTSIVVAGTHGKTTTT
CCCCCEEEEECHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHH
SLAAWLLEAGGFLPGFLIGGIPENFGDGCRPSGLSEPGFFVTEGDEYDSAFFDKRSKFLH
HHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEE
YRPDIAIINNVEFDHADIFDSLDDIKKSFCLLVNLVPSSGLLIVNGDDPNSMEVAAKAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCHHHHHHHHHH
RVETFGMNGTADWTATDITTDAESTVFTVVHNGEAMGRVSVPLFGNYNVMNALAATAAAI
EEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEECEEECCCHHHHHHHHHHHHH
RAGVSFESITKGLAKFKRPKRRMELVGEYAGGVTLIEDFAHHPTAVRLTLGAIAEHYPGR
HHCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCC
RIVACFEPRSNTTTRNIFQHELTECFDDASVVVLGRVNRPDRYAPEKRLDAAQLCHELEA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCHHHHHHHHHH
KGKRVFAAGDDDYPNDIVQFIKAEQQPGDVVVLLSNGSFNGLKTLLAESFEKKS
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNDFSAENLK
CEEEEEEECHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHHCCCCEEEEECCCCHHHC
SASPDLVVVGNAISRGNPDLEYALDTHMELISMPELVRRELIGRHTSIVVAGTHGKTTTT
CCCCCEEEEECHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHH
SLAAWLLEAGGFLPGFLIGGIPENFGDGCRPSGLSEPGFFVTEGDEYDSAFFDKRSKFLH
HHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEE
YRPDIAIINNVEFDHADIFDSLDDIKKSFCLLVNLVPSSGLLIVNGDDPNSMEVAAKAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCHHHHHHHHHH
RVETFGMNGTADWTATDITTDAESTVFTVVHNGEAMGRVSVPLFGNYNVMNALAATAAAI
EEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEECEEECCCHHHHHHHHHHHHH
RAGVSFESITKGLAKFKRPKRRMELVGEYAGGVTLIEDFAHHPTAVRLTLGAIAEHYPGR
HHCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCC
RIVACFEPRSNTTTRNIFQHELTECFDDASVVVLGRVNRPDRYAPEKRLDAAQLCHELEA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCHHHHHHHHHH
KGKRVFAAGDDDYPNDIVQFIKAEQQPGDVVVLLSNGSFNGLKTLLAESFEKKS
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]