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The map label for this gene is thiC [H]

Identifier: 193213185

GI number: 193213185

Start: 1686198

End: 1687865

Strand: Direct

Name: thiC [H]

Synonym: Cpar_1540

Alternate gene names: 193213185

Gene position: 1686198-1687865 (Clockwise)

Preceding gene: 193213182

Following gene: 193213191

Centisome position: 73.66

GC content: 63.67

Gene sequence:

>1668_bases
ATGAATCCAGAAAGCGCATCCTGCCCCGACAGGCATTTTTTCGGCCCCGCATCATCCCGCATGACCGTCAATGGCACCAT
CTACCCGATTGAAGTCGGCATGCGCAAGGTAGCCCTCAAGAGAACCTACGAGTGCAAGGGCGAGCCGTTCAACTCGATGC
CGCTGTACGACACGAGCGGCCCGTTCGGCGATCCCAACGGTGAGCACGATGTGCGCAAGGGACTTGAACCGGTTCGCGAC
CGCTGGGGCTTCGATAACGGCTCGGCAGAGTCGACGAAGGGTGAACTGTCGATGAGCGAGCGCAAGCCACGCGTGGCGAA
ATCGGGTGAGGCGGTCACGCAGATGCACTTCGCCCGCAAAGGCGTCATCACCCCGGAGATGGAGTACGTGGCGATCCGCG
AGAATCAGGCGCTGGAGGAGTGGATCGAAAAGTGCGGCGGCAAACCGGTCACGCCGGAGATGGTGCGCGATGAGGTGGCG
AAGGGACGGGCGATCATTCCGGCCAACATCAACCACCCGGAGATCGAGCCGATGATTATCGGGCGCAACTTCCGGGTCAA
GATCAACGCCAACATCGGCAACTCGGCGCTCGGCTCGTCGATTGATGAGGAGGTCGAAAAGGCGGTCTGGGCCTGCCGCT
GGGGCGCGGACACGGTGATGGATCTGAGCACGGGCAAGAACATCCACCAGACGCGAGAATGGATTCTGCGCAACTCGCCG
GTGCCGATCGGCACAGTGCCGCTCTACCAGGCGCTCGAAAAAGTGGGTGGCAAAGCCGAGGAGCTGAGCTGGGAGGTCTA
TCGCGACACCCTCGTCGAACAGGCCGAGCAGGGGGTAGACTACTTCACCATCCACTCGGGCATTCTCGCCGCCACGCTGC
CCGACGCCGAAGCGCGGCAGACCGGCATCGTTTCGCGCGGCGGTTCAATCATGGCCCGCTGGTGTCGCGCCCACAACCAG
GAGAACTTCCTCTTCACCCACTTCGACGACATCTGCGACATCCTCCGCAGCTACGATGTGGCGGTCTCGCTCGGAGACGC
ACTCCGCCCCGGCTCCATCGGCGACGCCAACGACGCCGCGCAGTTCGGCGAGCTGAAAACCCTCGGCGAGCTGACGCTCC
GCGCCTGGGAGCGCGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGCTGCACATGATCCGCGAGAACATGGAG
CTGCAGCTCAAGCACTGCCACGAAGCGCCGTTCTACACCCTCGGCCCGCTCGTGACCGACGTGGCTGCGGGATATGACCA
CATCAACTCGGCCATCGGCGGTACACTCATCGCCAGCCTCGGCTGCTCGATGCTCTGCTACGTCACGCCGAAAGAGCACC
TCGGCCTGCCCGACCGCGACGACGTGCGCGAAGGCGTCATCGTCCACCGCGTTGCAGCCCACGCCGCCGACCTCGCCAAG
GGAAGCCACACCGCCTGGCTGCGCGACGAGCTGATGAGCAAGGCCCGCTACGCCTTCGAGTGGGAAGACCAGTTCAGCCT
GGCGCTCGATCCGCTCAAGACAAGGCAGATCCACGCCCAGAACATCGCCGCTACCGGTGATACCGCCGCCACTGCCAAAT
TCTGCACCATGTGCGGCCCCGACTTCTGCTCGATGAAAAGGTCGCAGGAAACGACTGCAGGGATGTAA

Upstream 100 bases:

>100_bases
TGCTGACGCAAGGAAAGATGAAGCAAGACCTCCCCAATTGCTCTGCTTCACCATCCCTCTACGGCTGACACGCCCTGTGT
TCCTTTTTCAGACCCGTTCC

Downstream 100 bases:

>100_bases
CTATTTACCCCCCCCCTTTCCCCCAAAAAAGCAAAGGCTGCCCGTGGCTAACAGGCAGCCTTTGAAAAGCTGAATTTCCG
ACCTTCTTCTTTACCCCGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNPESASCPDRHFFGPASSRMTVNGTIYPIEVGMRKVALKRTYECKGEPFNSMPLYDTSGPFGDPNGEHDVRKGLEPVRD
RWGFDNGSAESTKGELSMSERKPRVAKSGEAVTQMHFARKGVITPEMEYVAIRENQALEEWIEKCGGKPVTPEMVRDEVA
KGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWACRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHNQ
ENFLFTHFDDICDILRSYDVAVSLGDALRPGSIGDANDAAQFGELKTLGELTLRAWERDVQVMIEGPGHVPLHMIRENME
LQLKHCHEAPFYTLGPLVTDVAAGYDHINSAIGGTLIASLGCSMLCYVTPKEHLGLPDRDDVREGVIVHRVAAHAADLAK
GSHTAWLRDELMSKARYAFEWEDQFSLALDPLKTRQIHAQNIAATGDTAATAKFCTMCGPDFCSMKRSQETTAGM

Sequences:

>Translated_555_residues
MNPESASCPDRHFFGPASSRMTVNGTIYPIEVGMRKVALKRTYECKGEPFNSMPLYDTSGPFGDPNGEHDVRKGLEPVRD
RWGFDNGSAESTKGELSMSERKPRVAKSGEAVTQMHFARKGVITPEMEYVAIRENQALEEWIEKCGGKPVTPEMVRDEVA
KGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWACRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHNQ
ENFLFTHFDDICDILRSYDVAVSLGDALRPGSIGDANDAAQFGELKTLGELTLRAWERDVQVMIEGPGHVPLHMIRENME
LQLKHCHEAPFYTLGPLVTDVAAGYDHINSAIGGTLIASLGCSMLCYVTPKEHLGLPDRDDVREGVIVHRVAAHAADLAK
GSHTAWLRDELMSKARYAFEWEDQFSLALDPLKTRQIHAQNIAATGDTAATAKFCTMCGPDFCSMKRSQETTAGM
>Mature_555_residues
MNPESASCPDRHFFGPASSRMTVNGTIYPIEVGMRKVALKRTYECKGEPFNSMPLYDTSGPFGDPNGEHDVRKGLEPVRD
RWGFDNGSAESTKGELSMSERKPRVAKSGEAVTQMHFARKGVITPEMEYVAIRENQALEEWIEKCGGKPVTPEMVRDEVA
KGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWACRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHNQ
ENFLFTHFDDICDILRSYDVAVSLGDALRPGSIGDANDAAQFGELKTLGELTLRAWERDVQVMIEGPGHVPLHMIRENME
LQLKHCHEAPFYTLGPLVTDVAAGYDHINSAIGGTLIASLGCSMLCYVTPKEHLGLPDRDDVREGVIVHRVAAHAADLAK
GSHTAWLRDELMSKARYAFEWEDQFSLALDPLKTRQIHAQNIAATGDTAATAKFCTMCGPDFCSMKRSQETTAGM

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=572, Percent_Identity=58.9160839160839, Blast_Score=679, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 61269; Mature: 61269

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPESASCPDRHFFGPASSRMTVNGTIYPIEVGMRKVALKRTYECKGEPFNSMPLYDTSG
CCCCCCCCCCCCCCCCCCCCEEECCEEEEEHHCHHHHHHHHHHCCCCCCCCCCCCCCCCC
PFGDPNGEHDVRKGLEPVRDRWGFDNGSAESTKGELSMSERKPRVAKSGEAVTQMHFARK
CCCCCCCCHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHCCCCHHHHHHHHHC
GVITPEMEYVAIRENQALEEWIEKCGGKPVTPEMVRDEVAKGRAIIPANINHPEIEPMII
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEE
GRNFRVKINANIGNSALGSSIDEEVEKAVWACRWGADTVMDLSTGKNIHQTREWILRNSP
CCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHH
TGIVSRGGSIMARWCRAHNQENFLFTHFDDICDILRSYDVAVSLGDALRPGSIGDANDAA
CCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QFGELKTLGELTLRAWERDVQVMIEGPGHVPLHMIRENMELQLKHCHEAPFYTLGPLVTD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
VAAGYDHINSAIGGTLIASLGCSMLCYVTPKEHLGLPDRDDVREGVIVHRVAAHAADLAK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GSHTAWLRDELMSKARYAFEWEDQFSLALDPLKTRQIHAQNIAATGDTAATAKFCTMCGP
CCCHHHHHHHHHHHHHCEEEECCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
DFCSMKRSQETTAGM
HHHHHCCCCHHHCCC
>Mature Secondary Structure
MNPESASCPDRHFFGPASSRMTVNGTIYPIEVGMRKVALKRTYECKGEPFNSMPLYDTSG
CCCCCCCCCCCCCCCCCCCCEEECCEEEEEHHCHHHHHHHHHHCCCCCCCCCCCCCCCCC
PFGDPNGEHDVRKGLEPVRDRWGFDNGSAESTKGELSMSERKPRVAKSGEAVTQMHFARK
CCCCCCCCHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHCCCCHHHHHHHHHC
GVITPEMEYVAIRENQALEEWIEKCGGKPVTPEMVRDEVAKGRAIIPANINHPEIEPMII
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEE
GRNFRVKINANIGNSALGSSIDEEVEKAVWACRWGADTVMDLSTGKNIHQTREWILRNSP
CCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHH
TGIVSRGGSIMARWCRAHNQENFLFTHFDDICDILRSYDVAVSLGDALRPGSIGDANDAA
CCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QFGELKTLGELTLRAWERDVQVMIEGPGHVPLHMIRENMELQLKHCHEAPFYTLGPLVTD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
VAAGYDHINSAIGGTLIASLGCSMLCYVTPKEHLGLPDRDDVREGVIVHRVAAHAADLAK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GSHTAWLRDELMSKARYAFEWEDQFSLALDPLKTRQIHAQNIAATGDTAATAKFCTMCGP
CCCHHHHHHHHHHHHHCEEEECCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
DFCSMKRSQETTAGM
HHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]