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The map label for this gene is priA [H]

Identifier: 193212781

GI number: 193212781

Start: 1244114

End: 1246546

Strand: Direct

Name: priA [H]

Synonym: Cpar_1127

Alternate gene names: 193212781

Gene position: 1244114-1246546 (Clockwise)

Preceding gene: 193212780

Following gene: 193212782

Centisome position: 54.35

GC content: 60.87

Gene sequence:

>2433_bases
ATGCGCGCACAGGTTTACGTTGAAAGAATCTATCGGGACGAACCGTTCCTGTTGTCGGTGCCAGAGGCGCTGGAAGAGCG
TCTGCAGACCGGTTGTCAGGTGCTGGTGAAGCTCGGGGGACACAGGGCTTCGGCGTACATCGGCTACGTTGCGTCTCTGG
ATGAACAGCCAGGCGAGAATGCGGTAGACGGCGAGGTGCTGGACGTGCTCAACGACGGCAGGCCGGTACTGACACCACAG
ATGATGCGGTTGGCGCTCTGGATGTCGGATCACTATGTCGCCCGGCCCGTCGACTGCATCACCACCGCCCTTCCCGCGCC
GCTGCGGAACACGGTCGACGAGGTGATCGAGCTGTGCGATTTCCGTCTGGAAAGCCCGGAGTCCCGAATTAAAAGCACCG
ATTTGCGCCGGAAAATTCTGCAGATGCTGAGCCGCGACAAGCGCCTGACCGTTCGGCAGCTCCGCAAACGCCTCGGCAGA
AAGGGGCTCTACTCCACACTTGGCGAGCTCGAAAGAGGCGGGCTGATTAAAATCACCAAGCGCTTTGCTGAAACAAAGCC
GAGAACCGTCACGGCATGGCGGCTGAGCCCCACGCTCCCCGAAGAGCCAGAAAAACTGATCGCCCGCTCGCCGCGAAAGC
GCGAGGCGTTCGAGCTGCTCTTCGCTGAACCCGACAAGCCGCTCCGGGCCGAGGAAAGCGGCATTTCGAGAGCGGTACTG
AACGGACTGGTCGAGCTGGGGCTGGCCGAAAAAGTCGAAATCGCCGCGCCACTCAAGGAGGGGCTTCGCTTCGACGAAGT
GCAGAAGGAGATCACCGCGCTCAGCCGGCACCAGCAGCAGGCGCTCGACGCGCTGACCGGGTCGCTCGAAGAGGAGCGGT
TCCGGACGTTTCTGTTGCACGGCGTGACCGGCAGCGGCAAGACGCTGGTCTATATCGAGCTGCTGCGCCGGGTGCTCGAC
AGCGGCAAAACAGCTATCGTACTCGTCCCCGAAATCGCCCTCACCCCACAAACCGCGGCCCGCTTCCGCCACTATTTCGG
TGATGAAATCCAGATCATACACAGCGCCATGAGCGATCAGGAAAAGTACGAGGCGTGGCAACGGCTCAGTACGGGCAAGG
CGCGCATCGCGCTCGGCGCGCGCTCGACCCTGTTTGCACCACTGGAGAATGTCGGAGCGATTATCGTGGACGAGGAACAC
GACGGAGCATACAAGCAGGATCGCACACCACGCTACCACGCTCGCGACACGGCGGTGATGCGCGCCATGTTCGAAAACGC
CATCTGCGTGCTCGGCTCGGCCACACCATCGTTCGAGTCGTACCACAATGCCGTCGAAAGCAAATACGCCCTGCTCGAAC
TGCCCGAACGCATCGACCAGGCACAAATGCCCAAAATCGAGCTGGTCTGGATGCCCGGCAGTCAGCGCGTCACGCCGTCG
ATCTCCGGAGCGCTGTACGACGCAATCCGCGAACGCCTCAAGCGCGACGAGCAGGTCATCCTCTTGCAAAACCGCCGCGG
CTTTGCGGGCAGCCTGCTCTGCCTCGAATGCGGCCACACCCCGCAATGCAGCCACTGCAACATCCCGCTGGTCTACCATT
CGACCGACCGGACGCTCCGCTGCCACTACTGCGGCCACATCGAGCCGTTCCGCGAAACCTGCCCCTCGTGCAAATCCGGC
AATCTGTTCTACAAAAGCAGCGGCACAGAGCGCATCGAGGAGGAGCTGGGTGAACTGTTTCCGGATGAGAAGATTTTGCG
GATGGACATCGACACCACCTCTACCAAAGACGCCCACGCCACGATTCTGACCGCCTTCCGCAACAAAACCGCTCGGATTC
TCCTCGGCACCCAGATGGTGGCCAAGGGGCTGGACTTTCCGGACGTCACGCTGGTTGGCGTGCTGATGGCCGACATAGGC
CTGAACCTGCCGGACTTCCGCGCCTCGGAGCGCATCTACTCGCTGCTGACGCAGGTGTCGGGACGAGCAGGACGAGCATC
GAGACCTGGCGAAGTGCTGCTTCAACTCTACAACCGCGACAACGAGCTGTTCAGCCACGTACTGAAAAGCGACTACCGGC
ATTTCTTCGAAGCCGAGATGCTCACCCGCAAAGAGCTTGGCTATCCGCCTTTTTCGAGACTCATCAAGTTCGAGTGCTCC
TCACCCTCCGAACAGGCCGCCGAACGCGGCGCAATGGCGCTCCGGGAAAAAATCGAACCGGTCGTAACCGAAAAGATCGG
CACGATTCTCGGCCCCGCGCCGGCAGGTATGATGAAAATGAAAGGGCGGTTCCGCTTTCAGCTCATCCTGAAACTCTTCG
GCGTCCGTTTGCCAAGCTCGCTCCTCAGACAGGTACAGTACGACGCACTCGGCCTTTTCCGAGGTGACAACCTGGTCATC
ACCGTCGATGTCGATCCCCAGCATCTTCTGTAA

Upstream 100 bases:

>100_bases
GGAAAAAAGCCAGTTGTTATGACTGGCTTTTTTTGTTTATATGACAACGATCGAACTCTCTTTTTGCTGATGAAACATCC
GCTCTTGAACAACCTCCTCG

Downstream 100 bases:

>100_bases
AACGCTTTTTTTGTCGTAAATTTCTGCGAAACCGGTTACAACGCAGAAATAATCAACCCGAAATCCATGACAGAGTTTCA
GTGGAACACCAGTTACCTTA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MRAQVYVERIYRDEPFLLSVPEALEERLQTGCQVLVKLGGHRASAYIGYVASLDEQPGENAVDGEVLDVLNDGRPVLTPQ
MMRLALWMSDHYVARPVDCITTALPAPLRNTVDEVIELCDFRLESPESRIKSTDLRRKILQMLSRDKRLTVRQLRKRLGR
KGLYSTLGELERGGLIKITKRFAETKPRTVTAWRLSPTLPEEPEKLIARSPRKREAFELLFAEPDKPLRAEESGISRAVL
NGLVELGLAEKVEIAAPLKEGLRFDEVQKEITALSRHQQQALDALTGSLEEERFRTFLLHGVTGSGKTLVYIELLRRVLD
SGKTAIVLVPEIALTPQTAARFRHYFGDEIQIIHSAMSDQEKYEAWQRLSTGKARIALGARSTLFAPLENVGAIIVDEEH
DGAYKQDRTPRYHARDTAVMRAMFENAICVLGSATPSFESYHNAVESKYALLELPERIDQAQMPKIELVWMPGSQRVTPS
ISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLECGHTPQCSHCNIPLVYHSTDRTLRCHYCGHIEPFRETCPSCKSG
NLFYKSSGTERIEEELGELFPDEKILRMDIDTTSTKDAHATILTAFRNKTARILLGTQMVAKGLDFPDVTLVGVLMADIG
LNLPDFRASERIYSLLTQVSGRAGRASRPGEVLLQLYNRDNELFSHVLKSDYRHFFEAEMLTRKELGYPPFSRLIKFECS
SPSEQAAERGAMALREKIEPVVTEKIGTILGPAPAGMMKMKGRFRFQLILKLFGVRLPSSLLRQVQYDALGLFRGDNLVI
TVDVDPQHLL

Sequences:

>Translated_810_residues
MRAQVYVERIYRDEPFLLSVPEALEERLQTGCQVLVKLGGHRASAYIGYVASLDEQPGENAVDGEVLDVLNDGRPVLTPQ
MMRLALWMSDHYVARPVDCITTALPAPLRNTVDEVIELCDFRLESPESRIKSTDLRRKILQMLSRDKRLTVRQLRKRLGR
KGLYSTLGELERGGLIKITKRFAETKPRTVTAWRLSPTLPEEPEKLIARSPRKREAFELLFAEPDKPLRAEESGISRAVL
NGLVELGLAEKVEIAAPLKEGLRFDEVQKEITALSRHQQQALDALTGSLEEERFRTFLLHGVTGSGKTLVYIELLRRVLD
SGKTAIVLVPEIALTPQTAARFRHYFGDEIQIIHSAMSDQEKYEAWQRLSTGKARIALGARSTLFAPLENVGAIIVDEEH
DGAYKQDRTPRYHARDTAVMRAMFENAICVLGSATPSFESYHNAVESKYALLELPERIDQAQMPKIELVWMPGSQRVTPS
ISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLECGHTPQCSHCNIPLVYHSTDRTLRCHYCGHIEPFRETCPSCKSG
NLFYKSSGTERIEEELGELFPDEKILRMDIDTTSTKDAHATILTAFRNKTARILLGTQMVAKGLDFPDVTLVGVLMADIG
LNLPDFRASERIYSLLTQVSGRAGRASRPGEVLLQLYNRDNELFSHVLKSDYRHFFEAEMLTRKELGYPPFSRLIKFECS
SPSEQAAERGAMALREKIEPVVTEKIGTILGPAPAGMMKMKGRFRFQLILKLFGVRLPSSLLRQVQYDALGLFRGDNLVI
TVDVDPQHLL
>Mature_810_residues
MRAQVYVERIYRDEPFLLSVPEALEERLQTGCQVLVKLGGHRASAYIGYVASLDEQPGENAVDGEVLDVLNDGRPVLTPQ
MMRLALWMSDHYVARPVDCITTALPAPLRNTVDEVIELCDFRLESPESRIKSTDLRRKILQMLSRDKRLTVRQLRKRLGR
KGLYSTLGELERGGLIKITKRFAETKPRTVTAWRLSPTLPEEPEKLIARSPRKREAFELLFAEPDKPLRAEESGISRAVL
NGLVELGLAEKVEIAAPLKEGLRFDEVQKEITALSRHQQQALDALTGSLEEERFRTFLLHGVTGSGKTLVYIELLRRVLD
SGKTAIVLVPEIALTPQTAARFRHYFGDEIQIIHSAMSDQEKYEAWQRLSTGKARIALGARSTLFAPLENVGAIIVDEEH
DGAYKQDRTPRYHARDTAVMRAMFENAICVLGSATPSFESYHNAVESKYALLELPERIDQAQMPKIELVWMPGSQRVTPS
ISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLECGHTPQCSHCNIPLVYHSTDRTLRCHYCGHIEPFRETCPSCKSG
NLFYKSSGTERIEEELGELFPDEKILRMDIDTTSTKDAHATILTAFRNKTARILLGTQMVAKGLDFPDVTLVGVLMADIG
LNLPDFRASERIYSLLTQVSGRAGRASRPGEVLLQLYNRDNELFSHVLKSDYRHFFEAEMLTRKELGYPPFSRLIKFECS
SPSEQAAERGAMALREKIEPVVTEKIGTILGPAPAGMMKMKGRFRFQLILKLFGVRLPSSLLRQVQYDALGLFRGDNLVI
TVDVDPQHLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=569, Percent_Identity=40.4217926186292, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91310; Mature: 91310

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQVYVERIYRDEPFLLSVPEALEERLQTGCQVLVKLGGHRASAYIGYVASLDEQPGEN
CCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
AVDGEVLDVLNDGRPVLTPQMMRLALWMSDHYVARPVDCITTALPAPLRNTVDEVIELCD
CCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
FRLESPESRIKSTDLRRKILQMLSRDKRLTVRQLRKRLGRKGLYSTLGELERGGLIKITK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
RFAETKPRTVTAWRLSPTLPEEPEKLIARSPRKREAFELLFAEPDKPLRAEESGISRAVL
HHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
NGLVELGLAEKVEIAAPLKEGLRFDEVQKEITALSRHQQQALDALTGSLEEERFRTFLLH
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GVTGSGKTLVYIELLRRVLDSGKTAIVLVPEIALTPQTAARFRHYFGDEIQIIHSAMSDQ
CCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCH
EKYEAWQRLSTGKARIALGARSTLFAPLENVGAIIVDEEHDGAYKQDRTPRYHARDTAVM
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
RAMFENAICVLGSATPSFESYHNAVESKYALLELPERIDQAQMPKIELVWMPGSQRVTPS
HHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
ISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLECGHTPQCSHCNIPLVYHSTDRTLR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEE
CHYCGHIEPFRETCPSCKSGNLFYKSSGTERIEEELGELFPDEKILRMDIDTTSTKDAHA
EEECCCCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
TILTAFRNKTARILLGTQMVAKGLDFPDVTLVGVLMADIGLNLPDFRASERIYSLLTQVS
HHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GRAGRASRPGEVLLQLYNRDNELFSHVLKSDYRHFFEAEMLTRKELGYPPFSRLIKFECS
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECC
SPSEQAAERGAMALREKIEPVVTEKIGTILGPAPAGMMKMKGRFRFQLILKLFGVRLPSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHH
LLRQVQYDALGLFRGDNLVITVDVDPQHLL
HHHHHHHHHHEEEECCCEEEEEECCHHHCC
>Mature Secondary Structure
MRAQVYVERIYRDEPFLLSVPEALEERLQTGCQVLVKLGGHRASAYIGYVASLDEQPGEN
CCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
AVDGEVLDVLNDGRPVLTPQMMRLALWMSDHYVARPVDCITTALPAPLRNTVDEVIELCD
CCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
FRLESPESRIKSTDLRRKILQMLSRDKRLTVRQLRKRLGRKGLYSTLGELERGGLIKITK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
RFAETKPRTVTAWRLSPTLPEEPEKLIARSPRKREAFELLFAEPDKPLRAEESGISRAVL
HHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
NGLVELGLAEKVEIAAPLKEGLRFDEVQKEITALSRHQQQALDALTGSLEEERFRTFLLH
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GVTGSGKTLVYIELLRRVLDSGKTAIVLVPEIALTPQTAARFRHYFGDEIQIIHSAMSDQ
CCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCH
EKYEAWQRLSTGKARIALGARSTLFAPLENVGAIIVDEEHDGAYKQDRTPRYHARDTAVM
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
RAMFENAICVLGSATPSFESYHNAVESKYALLELPERIDQAQMPKIELVWMPGSQRVTPS
HHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
ISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLECGHTPQCSHCNIPLVYHSTDRTLR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEE
CHYCGHIEPFRETCPSCKSGNLFYKSSGTERIEEELGELFPDEKILRMDIDTTSTKDAHA
EEECCCCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
TILTAFRNKTARILLGTQMVAKGLDFPDVTLVGVLMADIGLNLPDFRASERIYSLLTQVS
HHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GRAGRASRPGEVLLQLYNRDNELFSHVLKSDYRHFFEAEMLTRKELGYPPFSRLIKFECS
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECC
SPSEQAAERGAMALREKIEPVVTEKIGTILGPAPAGMMKMKGRFRFQLILKLFGVRLPSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHH
LLRQVQYDALGLFRGDNLVITVDVDPQHLL
HHHHHHHHHHEEEECCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]